High speed & high resolution
COSMOSIL® non-aqueous GPC columns 5GPC-60, 5GPC-100 and 5GPC-300 can provide high resolution, high-speed analysis. GPC packing materials are 5µm diameter particles of totally porous spherical silica gel. Without swelling they display excellent stability against pressure. For GPC, all samples are eluted within t0. The t0 value for a COSMOSIL® 5GPC 4.6mmI.D.x250mm column is about three minutes at a flow rate of 1.0ml/min; meaning that the analysis is completed within about three minutes. COSMOSIL® 5GPC column therefore require less solvent. This is important, especially when expensive solvents such as 1,1,1,3,3,3-hexafluoro-2-propanol are used. In each molecular weight range, the GPC columns provide linear correlation between log MW and the elution volume. The large pore volume results in high peak capacity. Higher efficiencies are obtained with multiple columns. Coupled columns (GPC-60 and GPC-100, or GPC-100 and GPC-300) are frequently employed to meet the wide molecular weight range of samples.
Column characteristics
Type Particle size Pore size MW range COSMOSIL® 5GPC-60 5µm 60Å 300-15,000 COSMOSIL® 5GPC-100 5µm 100Å 1000-50,000 COSMOSIL® 5GPC-300 5µm 300Å 17,000-300,000
Recommended solvents and optimal temperature - Solvents
Saturated hydrocarbons and the solvents specified below can be used with the GPC column. The use of solvents and mobile phases containing water should be avoided in order to prevent deterioration of column efficiency. Solvents from the halogen series may corrode the stainless steel tubing and fittings of the HPLC system. After using these solvents, wash the column with solvent not containing halogen ions. Column washing and changing of mobile phase should be conducted with low flow rate. Flow rate of less than 0.5ml/min for 4.6mmI.D. columns and less than 1.5ml/min for 10mmI.D. columns are recommended.
-Temperature
Run the separation at a column temperature of 50°C lower than the boiling point of the mobile phase in order to prevent bubble formation.
Organic solvents for non-aqueous GPC columns
SOLVENT Physical property of the solvent Toxicity Boiling point °C Viscosity (20°C)cp. Refractive index(20°C) Flash Point°C LD50mg/kg L(ct)50ppm 1,1,1,3,3,3-Hexafluoro-2-propanol 59 - 1.2700 - - - Tetrahydrofuran 66 0.51(25°C) 1.4070 14 hazardous 200 1,2,4-Trichlorobenzene 213 1.89(25°C) 1.5717 99 hazardous 75 o- Dichlorobenzene 180 1.26 1.5515 66 500 50 Toluene 110.6 0.59 1.4961 4 hazardous 6000-200 N,N- Dimethylformamide 153 0.90 1.4280 58 hazardous 100-10 Dichloromethane 40.1 0.30 1.4242 - 3000 500 1,2-Dichloroethane 84 0.84 1.4443 13 770 50 N- Methylpyrrolidone 202 1.65 1.47 95.4 hazardous - m- Cresol 202.8 20.8 1.544 94 hazardous 5 Benzene 80.1 0.652 1.5011 27 7000 25 Dimethyl Sulioxide 189 - 1.477 95 hazardous - Tetrachloroethylene 121 - 1.505 - hazardous 100 o- Chlorophenol 175.5 4.11 1.5473 - 670 dangerous CarbonTetrachloride 76.8 0.969 1.4607 - hazardous 10 Trifluoroethanol 73.6 1.20 (38°C) 1.291 40.6 240 4600 Chloroform 61.7 0.58 1.446 - hazardous 50 Acetone 56.2 0.316 1.359 -17.8 4-8 500
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Ordering information
Product name Size Code No. End-fitting COSMOSIL® 5GPC-60 Packed Column 4.6mmI.D.x 250mm 37830-61 Waters type 10mmI.D.x 250mm 37826-31 COSMOSIL® 5GPC-100 Packed Column 4.6mmI.D.x 250mm 39732-91 10mmI.D.x 250mm 39744-41 COSMOSIL® 5GPC-300 Packed Column 4.6mmI.D.x 250mm 39736-51 10mmI.D.x 250mm 39752-31
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