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The 2mm I.D. semi-microbore columns of C18 phases are available in
the COSMOSIL® series. Compared with 4.6mm I.D. columns, the semi-microbore columns are 5 times more sensitive and reduce
the solvent consumption by 80%.
The COSMOSIL® semi-microbore columns perform best when used with micro analysis HPLC equipment including tubing, injector
and detector cell.
The semi-microbore columns can replace existing COSMOSIL® analytical columns. Available packing materials are
C18-MS, C18-AR-II and C18-AR-300.
Please consult the table and the applications below for selection guidance.
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Material characteristics |
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5C18-MS-II |
5C18-AR-II |
5C18-AR-300 |
| Silica gel |
high purity spherical porous silica |
| Average particle size |
5µm |
| Average pore size |
approx. 120Å |
approx. 300Å |
| Specific surface area |
approx. 300m2/g |
approx. 150m2/g |
| Stationary phase |
monomeric type |
polymeric type |
| End capping treatment |
near-perfect treatment |
| Carbon content |
approx. 16% |
approx. 17% |
approx. 12% |
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Sample load capacity |
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 :4.6mm I.D. column  : 2.0mm I.D. COSMOSIL® 5C18-MS-II column |
Injection volume |
| Dead volume greatly affects the semi-microbore column performance.Use of a micro injector is highly recommended. In order to avoid peak broadening, the injection volume should be less then 1.0µl. |
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Recommended operating procedures |
- Flow rate must be less than 0.2 ml/min.
- Pressure must be less than 15.0MPa\
- Filter solvents and samples with 0.5µm filters.
- When you use a conventional HPLC system,the theoretical plate number may be lowered by about 30%
- Use of micro analysis HPLC equipment including tubing,injector and detector cell is highly recommended.
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Comparison with 4.6mml.D. column
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The COSMOSIL® semi-microbore columns are taking over the high performance of COSMOSIL® series. The semi-microbore columns can be used in the same applications of other analytical COSMOSIL® columns.
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Ordering information |
| Product name |
Size |
Code No. |
End-fitting |
| COSMOSIL® 5C18-MS-II Packed Column |
2.0mmI.D.×150mm |
38025-91 |
Waters type |
| COSMOSIL® 5C18-AR-II Packed Column |
2.0mmI.D.×150mm |
37992-51 |
| COSMOSIL® 5C18-AR-300 Packed Column |
2.0mmI.D.×150mm |
37991-61 |
| COSMOSIL® 5PYE Packed Column |
2.0mmI.D.×100mm |
34501-61 |
| 2.0mmI.D.×150mm |
38042-61 |
| 2.0mmI.D.×250mm |
34450-31 |
| COSMOSIL® 5NPE Packed Column |
2.0mmI.D.×100mm |
34500-71 |
| 2.0mmI.D.×150mm |
34328-51 |
| 2.0mmI.D.×250mm |
34379-91 |
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| (C)2004 Nacalai Tesque, Inc. E-mail: info.intl@nacalai.co.jp |