Hydrophobic interaction chromatography (HIC) is an effective method for purification and separation of proteins (especially enzymes) based on differences in their surface hydrophobicity. Since this method does not use organic solvents like reversed phase chromatography, there is only a little loss in enzyme activity and the tercially structure of proteins (denaturation). COMSOSIL 5HIC is a packed column for hydrophobic interaction chromatography on silica gel base.

Material characteristics

COMSOSIL 5HIC
Silica gel	high purity porous spherical silica
Average particle size	5µm
Average pore size	approx. 120Å
Specific surface area	approx. 150m²/g

Characteristics

No need for organic solvents.( → Protein is not denatured. )
Allows high pressure and high flow rate because of silica gel base.
No problem with swelling like with polymer gel bases.
Economic solution.

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Ordering information

Product name	Size	Code No.	End-fitting
COSMOSIL^® 5HIC Packed Column	4.6mmI.D.× 50mm	04263-21	Waters type