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Melamine analysis by COSMOSIL HILIC

The followings are melamine analysis by HPLC and LC/MS/MS using COSMOSIL HILIC.


Melamine analysis by COSMOSIL HILIC



LC/MS/MS (COSMOSIL HILIC　2.0mml.D.×150mm)




Column COSMOSIL HILIC 2.0 mm I.D. x 150 mm
Mobile Phase A:acetonitrile + 10mmol/l ammonium acetate
B:water + 10mmol/l ammonium acetate
Gradient
time
(min)		 flow rate
(µl/min)		 %A %B
int 250 97 3
5.0 250 20 80
5.5 250 3 97
5.6 250 97 3
10.0 250 97 3
Temperature 40°C
Detection MRM condition (ESI-POS)
Q1 Q3
127 85
127 68
127 60
Injection volume Melamine(10ppb / 10µl / inj)
LC/MS/MS data is provided by customer.

Melamine analysis (861KB)


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