COSMOSIL HILIC is a new column for hydrophilic interaction chromatography with Triazole bonded silica packing material. The hydrophilic interaction chromatography is a variation of normal phase chromatography where a polar stationary phase is used with a mobile phase which contains a high concentration of organic solvent and a low concentration of aqueous eluent. The main retention mechanism is the partitioning of the polar analytes between the polar stationary and the non-polar mobile phase. As it is also called "aqueous normal phase", the elution order is similar to that of normal phase and the sample elution is in the order of increasing hydrophilicity. Without using ion-pair reagent COSMOSIL HILIC retains highly polar analytes that would not be retained in reversed phase chromatography. It also shows a weak anion-exchange mechanism with the positively charged stationary phase, thus acidic compound is strongly retained.

Melamine analysis by COSMOSIL HILIC

Features of COSMOSIL HILIC

Retains highly polar compounds that would not be retained in C18 columns.
Ion-pair reagent is not required
Anion-exchange results in strong retention for acidic compounds.
Enhanced sensitivity in LC/MS

Comparison with Hydrophobicity
COSMOSIL HILIC can separate Glycine and Glycylglycine without ion-pair reagent. Although C18 column can separate
them with ion-pair reagents, there are some disadvantages such as column equilibration, preparation of mobile phase and column deterioration.

Material characteristics

Different interactions

The retention Mechanism of COSMOSIL HILIC is the combination of hydrophilic interaction and anion exchange, and the
retention can be controlled by changing the mobile phase.

Analysis data

Melamine analysis by COSMOSIL HILIC

Combination with C18 columns

Selection of mobile phase

Following are the recommended mobile phases for different samples.

Acetonitrile / Water = 90 / 10
Acetonitrile / 10mmol/l CH3COONH4 = 90 / 10
Acetonitrile / 10mmol/l CH3COONH4 = 70 / 30
Acetonitrile / 10mmol/l CH3COONH4 = 50 / 50
(not eluted) → Acetonitrile / 10mmol/l Phosphatase buffer (pH7.0) = 50 / 50

COSMOSIL Chromatogram Index

COMSOSIL HILIC Chromatogram Index, which includes 154 chromatograms using COSMOSIL HILIC, is now available. This index is useful for optimizing analytical conditions for hydrophilic interaction chromatography.

COSMOSIL HILIC (PDF file 337KB)

COSMOSIL Chromatogram Index (PDF file 3.1MB)

Poster Presentation


HPLC 2008 (PDF file) May 10-16, 2008 Baltimore, MD, USA	HPLC 2007 (PDF file) June 17-21, 2007 Ghent, Belgium

Ordering information

Product name	Size	Code No.
COSMOSIL^® HILIC Guard Column	4.6mml.D.x 10mm	07055-61
	10mml.D.x 20mm	07058-31
	20mmI.D. x 20mm	07854-91
	20mmI.D. x 50mm	07873-41
	28mmI.D. x 50mm	07874-31
COSMOSIL^® HILIC Packed Column	1.0mml.D.x 150mm	07869-11
	1.0mml.D.x 250mm	07870-71
	2.0mml.D.x 50mm	07052-91
	2.0mml.D.x 150mm	07054-71
	2.0mml.D.x 250mm	07489-91
	3.0mml.D.x 150mm	07871-61
	3.0mml.D.x 250mm	07872-51
	4.6mml.D.x 150mm	07056-51
	4.6mml.D.x 250mm	07057-41
	10mml.D.x 250mm	07059-21
	20mml.D.x 250mm	07060-81
	28mml.D.x 250mm	07875-21