COSMOSIL πNAP is a Reversed Phase HPLC Column with Naphthalene bonded
silica packing material. Naphthalethyl group has two aromatic rings and
has strong π-π interaction. This column offers improved separation of
compounds such as positional isomers that are difficult to analyze with
Alkyl group bonded materials. |
|
Features of COSMOSIL πNAP |
- Naphthalene bonded silica packing material
- Stronger π-π interaction than Phenyl column
|
Material characteristics |
| Packing material |
COSMOSIL πNAP |
Structure of stationary phase |
| Silica gel |
high purity spherical porous silica |
 |
| Average particle size |
5µm |
| Average pore size |
approx. 120Å |
| Specific surface area |
approx. 300m2/g |
| Stationary phase |
Naphtylethyl group |
| End-capping treatment |
treated |
| Carbon content |
approx. 11% |
|
Comparison with Phenyl columns |
|
COSMOSIL πNAP with two aromatic rings has storonger π-π interactions than
Phenyl column which have one aromatic ring. Stationary phase with π electron
such as Phenyl and Naphthalene retain strongly Nitrobenzene which has more
π electrons than Benzene.
|
|
Application data |
●Position isomers of Tolunitrile |
5C18-MS-II
(40%Methanol) |
5PE-MS
(40%Methanol) |
πNAP
(50%Methanol) |
 |
|
| Column size |
4.6 mmI.D.×150 mm |
Sample |
| o-Tolunitrile |
(2.0µg) |
 |
| m-Tolunitrile |
(2.0µg) |
| p-Tolunitrile |
(1.0µg) |
|
| Flow rate |
1.0 ml/min |
| Temperature |
30 °C |
| Detection |
UV 254nm |
|
| πNAP enables the perfect separation of ortho- and para-position that can
not be separated enough by 5C18-MS-II or 5PE-MS. |
●Position isomers of Phthalonitrile |
5C18-MS-II
(30%Methanol) |
5PE-MS
(40%Methanol) |
πNAP
(50%Methanol) |
 |
|
| Column size |
4.6 mmI.D.×150 mm |
Sample |
| Phthalonitrile(o-isomer) |
(0.3µg) |
 |
| Isophthalonitrile(m-isomer) |
(3.0µg) |
| Terephthalonitrile(p-isomer) |
(0.15µg) |
|
| Flow rate |
1.0 ml/min |
| Temperature |
30 °C |
| Detection |
UV 254nm |
|
| πNAP nenables the perfect separation of position isomers of Phthalonitrile
that can not be separated enough by 5C18-MS-II or 5PE-MS. |
●Tocopherol |
5C18-MS-II
(95%Methanol) |
5PE-MS
(90%Methanol) |
πNAP
(90%Methanol) |
 |
|
| Column size |
4.6 mmI.D.×150 mm |
Sample |
| Tocopherol (α,β,γ,δ) |
(1.25µg each) |
 |
| |
α |
β |
γ |
δ |
| R1 |
Me |
Me |
H |
H |
| R2 |
Me |
H |
Me |
H |
| R3 |
Me |
Me |
Me |
Me |
|
|
| Flow rate |
1.0 ml/min |
| Temperature |
30 °C |
| Detection |
UV 295nm |
|
| πNAP nenables the perfect separation of β- and γ-Tocopherol that can not be separated by 5C18-MS-II or 5PE-MS. |
●Adrenal cortex hormone |
5C18-MS-II
(50%Methanol) |
5PE-MS
50%Methanol) |
πNAP
(60%Methanol) |
 |
|
| Column size |
4.6 mmI.D.×150 mm |
Sample |
| 1.Prednisolone |
(0.33µg) |
 |
| 2.Hydrocortisone |
(0.33µg) |
| 3.Cortisone |
(0.33µg) |
|
| Flow rate |
1.0 ml/min |
| Temperature |
30 °C |
| Detection |
UV 254nm |
|
| πNAP enables the perfect separation of Prednisolene and Hydrocortisone
which can not be separated enough with 5C18-MS-II due to their hydrophobic
similarity. |
●Isoflavone |
| 5C18-MS-II |
5PE-MS |
πNAP |
 |
|
| Column size |
4.6 mmI.D.×150 mm |
Sample |
| 1. Daidzin |
(0.05µg) |
| 2. Glycitin |
(0.05µg) |
| 3. Genistin |
(0.05µg) |
| 4. Daidzein |
(0.05µg) |
| 5. Glycitein |
(0.075µg) |
| 6. Genistein |
(0.025µg) |
|
| Mobile phase |
| A: |
Acetonitrile/20mmol/l Phosphate Buffer
(pH2.5)=10/90 |
| B: |
Acetonitrile/20mmol/l Phosphate Buffer
(pH2.5)=50/50 |
| B 0→100%/20min linear
gradien |
|
| Flow rate |
1.0 ml/min |
| Temperature |
30 °C |
| Detection |
UV 260nm |
|
The chromatograms above are 6 kinds of Isoflavone which belong to group
of polyphenol compoumds.
|
| ●Berberine |
| 5C18-MS-II |
5PE-MS |
πNAP |
 |
|
| Column size |
4.6 mmI.D.×150 mm |
Sample |
| 1.Palmatine Chloride |
(0.2µg) |
| 2.Berberine Hydrochloride |
(0.2µg) |
 |
| Mobile phase |
| 5C18-MS-II |
| :Methanol/20mmol/l Phosphate Buffer (pH2.5)=
40/60 |
|
| 5PE-MS |
| :Methanol/20mmol/l Phosphate Buffer (pH2.5)=
60/40 |
|
| πNAP |
| :Methanol/20mmol/l Phosphate Buffer (pH2.5)=
80/20 |
|
| Flow rate |
1.0 ml/min |
| Temperature |
30 °C |
| Detection |
UV 254nm |
|
| πNAP enables the perfect separation of impurities such as Palmatine in
Berberine chloride. |
Futher separation |
COSMOSIL PYE is a packed column with pyrene bonded silica packing material.
It has stronger π-π interaction than πNAP since pyrene has four atromatic
rings. For more information, please refer to website at COSMOSIL PYE more detalis
|
| ●Sterol |
5C18-MS-II
(98%Methanol) |
πNAP
(90%Methanol) |
PYE
(95%Methanol) |
 |
|
| Column size |
4.6 mmI.D.×150 mm |
Sample |
| 1. Cholesterol |
 |
| 2. Stigmasterol |
| 3. Campesterol |
| 4. Sitosterol |
|
| Flow rate |
1.0 ml/min |
| Temperature |
30 °C |
| Detection |
UV 210nm |
|
| PYE enables the perfect separation of Stigmasterol and Campesterol which can not be separated with 5C18-MS-II due to its
stronger π-π interaction. |
COSMOSIL πNAP (PDF2.2MB) |
Poster Presentation |
HPLC 2008 (PDF file)
May 10-16, 2008
Baltimore, MD, USA
|
Ordering information |
|
Product name
|
Size
|
Code No.
|
| COSMOSIL πNAP Guard Column |
4.6mml.D.×10mm |
08082-71 |
| 10mml.D.×20mm |
08087-21 |
| 20mml.D.×20mm |
08090-61 |
| 20mml.D.×50mm |
08091-51 |
| 28mml.D.×50mm |
08094-21 |
| COSMOSIL πNAP Packed Column |
1.0mml.D.×150mm |
08076-61 |
| 1.0mml.D.×250mm |
08077-51 |
| 2.0mml.D.×150mm |
08078-41 |
| 2.0mml.D.×250mm |
08079-31 |
| 3.0mml.D.×150mm |
08080-91 |
| 3.0mml.D.×250mm |
08081-81 |
| 4.6mml.D.×50mm |
08083-61 |
| 4.6mml.D.×100mm |
08084-51 |
| 4.6mml.D.×150mm |
08085-41 |
| 4.6mml.D.×250mm |
08086-31 |
| 10mml.D.×150mm |
08088-11 |
| 10mml.D.×250mm |
08089-01 |
| 20mml.D.×150mm |
08092-41 |
| 20mml.D.×250mm |
08093-31 |
| 28mml.D.×250mm |
08095-11 |
|
|
