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| COSMOSIL Cholester is a Reversed Phase HPLC Column with Cholestery bonded silica packing material, which provides equivalent hydrophobicity like that of traditional Alkyl group bonded silica packing materials (C18, C30). However, Cholester offers strong stereo selectivity for hydrophobic compounds to yield unique and reproducible separation patterns following the same analytical conditions used with other ODS columns. |
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Basic Nature
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Separation of Alkylbenzene (comparison with Hydrophobicity)
Cholester provides the same hydrophobicity as Alkyl group bonded types (C18, C30). So it is not necessary to change the analytical conditions when they are replaced by Cholester. |
| Cholester |
Traditional C18 |
Traditional C30 |
80Me001.gif) |
80Me002.gif) |
80Me003.gif) |
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| Condition |
Column size
Mobile phase
Flow rate
Temperature
Detection |
4.6mmI.D.x 150mm
Methanol : Water=80 : 20
1.0ml/min
30oC
UV254nm |
Sample
1. Benzene
2. Toluene
3. Ethylbenzene
4. Propylbenzene
5. Butylbenzene
6. Amylbenzene
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1.67µg
1.67µg
1.67µg
1.67µg
1.67µg
1.67µg |
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<<Click Separation Characteristics Click >> |
Application data
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| The unique rigid-structure offers excellent retention and separation of difficult analyties. |
Vitamin D |
| Cholester |
Traditional C18 |
Traditional C30 |
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| Condition |
Column size
Mobile phase
Flow rate
Temperature
Detection
Sample:
1. Vitamin D2
2. Vitamin D3 |
4.6mmI.D.x 150mm
Methanol : Water=80 : 20
1.0ml/min
30oC
UV265nm
0.3µg
0.1µg |
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Click Click |
Material characteristics
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| COSMOSIL® Cholester |
Silicagel
Average particle size
Average pore size
Specific surface area
Stationary phase
End capping treatment
Carbon content |
high purity porous spherical silica
5µm
approx. 120Å
approx. 300m2/g
Monomeric Cholesteryl group
near-perfect treatment
approx. 20% |
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Poster presentation
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HPLC 2007 (PDF file)
June 17-21, 2007
Ghent, Belgium
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Ordering information
Product name |
Size |
Code No. |
COSMOSIL® Cholester Guard Column |
4.6mml.D.×10mm |
05975-71 |
10mml.D.×20mm |
05978-41 |
20mml.D.×20mm |
05980-91 |
20mml.D.×50mm |
05981-81 |
28mml.D.×50mm |
05983-61 |
COSMOSIL® Cholester Packed Column |
1.0mml.D.×150mm |
05968-71 |
1.0mml.D.×250mm |
05969-61 |
2.0mml.D.×150mm |
05971-11 |
2.0mml.D.×250mm |
05972-01 |
3.0mml.D.×150mm |
05973-91 |
3.0mml.D.×250mm |
05974-81 |
4.6mml.D.×150mm |
05976-61 |
4.6mml.D.×250mm |
05977-51 |
10mml.D.×250mm |
05979-31 |
20mml.D.×250mm |
05982-71 |
28mml.D.×250mm |
05985-41 |
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| (C)2004 Nacalai Tesque, Inc. E-mail: info.intl@nacalai.co.jp |