COSMOSIL® 5CSP-HPR column was developed for enantiomeric separation based on ligand exchange chromatography (LEC). LEC for the separation of enantiomers was introduced by Davankov in 1973. This column directly separates the enatiomers (e.g. Α-amino acids, α-hydroxycarboxylic acids) having two polar functional groups in a near position, using mobile phase containing Cu (II) ions.
Material characteristics
COSMOSIL® 5CSP-HPR Silica gel high purity porous spherical silica gel Average particle size 5µm Average pore size approx. 120Å Specific surface area approx. 300m2/g
The chromatogram above shows enantiomeric separation of DL-Α-alanine. DL-α-alanine, detected by UV 254 nm upon complex formation with Cu (II) ions.
The chromatogram above shows enantiomeric separation of DL-α-selenomethionine which be used in structural analysis of proteins. Since DL-α-selenomethionine contains selenium (a heavy atom), it interacts with the stationary phase strongly, therefore the retention time becomes very long. In order to maintain suitable retention time, it is necessary to raise the CuSO4 concentration. In practice, on the 5CSP-HPR column the retention time can be controlled simply by changing the CuSO4 concentration. The addition of organic solvents like acetonitrile decreases the retention time as well.
Ordering information
Product name Size Code No. End-fitting COSMOSIL® 5CSP-HPR Guard Column 4.6mmI.D.× 10mm 04437-51 Waters type COSMOSIL® 5CSP-HPR Packed Column 4.6mmI.D.×150mm 04378-31 4.6mmI.D.×250mm 04680-51
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