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| COSMOSIL Cholester can often provide improved peak shapes and separatios than traditional Alkyl chain bonded types (C18, C30). |
| Stereo selectivity |
Positional isomers |
Geometrical isomers |
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Stereo selectivity
Carotene
|
| Cholester |
Traditional C18 |
Traditional C30 |
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| Condition |
|
Column size:
Mobile phase:
Flow rate:
Temperature:
Detection:
|
4.6mmI.D.x 150mm
THF : Methanol = 20 : 80
1.0ml/min
30°C
UV 470nm
|
Sample:
1. α-Carotene
2. β-Carotene |
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Positional isomer
Chloroacetophenone |
Cholester |
Traditional C18 |
Traditional C30 |
 |
|
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| Condition |
Column size:
Mobile phase:
Flow rate:
Temperature:
Detection: |
4.6mmI.D.x 150mm
Methanol : Water = 50 : 50
1.0ml/min
30°C
UV 254nm |
Sample:
o-Chloroacetophenone
m-Chloroacetophenone
p-Chloroacetophenone |
0.3μg
0.15μg
0.05μg |
 |
|
Methoxyphenol |
Cholester |
Traditional C18 |
Traditional C30 |
|
|
|
|
| Condition |
Column size:
Mobile phase:
Flow rate:
Temperature:
Detection: |
4.6mmI.D.x 150mm
Methanol : Water = 30 : 70
1.0ml/min
30°C
UV 254nm |
Sample:
o-Methoxyphenol
m-Methoxyphenol
p-Methoxyphenol |
3.3μg
3.3μg
3.3μg
|
 |
|
|
Chlorophenol |
Cholester |
Traditional C18 |
Traditional C30 |
|
|
|
|
|
| Condition |
Column size:
Mobile phase:
Flow rate:
Temperature:
Detection: |
4.6mmI.D.x 150mm
Methanol : Water = 30 : 70
1.0ml/min
30°C
UV 254nm |
Sample:
o-Chlorophenol
m-Chlorophenol
p-Chlorophenol |
2.0μg
2.0μg
4.0μg |
 |
|
|
Terphenyl |
Cholester |
Traditional C18 |
Traditional C30 |
|
 |
 |
|
|
| Condition |
Column size:
Mobile phase:
Flow rate:
Temperature:
Detection: |
4.6mmI.D.x 150mm
Methanol : Water = 90 : 10
1.0ml/min
30°C
UV 254nm |
Sample:
o-Terphenyl
m-Terphenyl
p-Terphenyl |
0.15μg
0.05μg
0.075μg |
 |
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|
Geometrical isomer
Stilbene |
Cholester |
Traditional C18 |
Traditional C30 |
|
|
|
|
|
| Condition |
Column size:
Mobile phase:
Flow rate:
Temperature:
Detection: |
4.6mmI.D.x 150mm
Acetonitrile : Water = 70 : 30
1.0ml/min
30°C
UV 254nm |
Sample:
1. cis-Stilbene
2. trans-Stilbene |
0.1μg
0.2μg |
 |
|
|
Vitamin A acid |
Cholester |
Traditional C18 |
Traditional C30 |
|
|
|
|
| Condition |
Column size:
Mobile phase:
Flow rate:
Temperature:
Detection:
Sample:
1. 13-cis-Retinoic Acid
2. all-trans-Retinoic Acid |
4.6mmI.D.x 150mm
Methanol : 20mmol/l Phosphate Buffer(pH2.5)=90 : 10
1.0ml/min
30°C
UV 350nm
0.04μg
0.04μg |
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