Conventionally Aminopropyl bounded stationary phases are used for liquid chromatographic analysis of mono- and oligosaccharides. General shortcomings of the conventional Aminopropyl bounded phases are tailing and adsorption of certain saccharides and general low durability (short active life) of these columns. These problems are addressed and solved by the novel COSMOSIL^® Sugar-D, resulting in better (sharper) separation and much improved durability.

Durability

The decrease of retention time was compared between COSMOSIL^® Sugar-D and conventional Aminopropyl bounded stationary phase under severe condition of 100% water as eluent between tests. The capacity factor did not decrease in case of COSMOSIL^® Sugar-D.

Eluent condition
Eluent Flow rate Temperature	: water : 1.0 ml/min : RT
Test condition
Column size Mobile phase Flow rate Temperature Detection Sample	: 4.6 mmI.D.×250 mm : acetonitrile:water=70:30 : 1.0 ml/min : 30°C : RI : Maltose

Absorption characteristic

Certain types of saccharides such as Arabinose or Galactose are partially or temporarily absorbed in conventional Aminopropyl stationary phases causing disappearance of sample or tailing. These problematic saccharides elute sharply and in proportion of the injection volume from the superior COSMOSIL^® Sugar-D.

Chromatogram

COSMOSIL^® Sugar-D

Aminopropyl type

Analitical curve

Condition
Column size : 4.6 mmI.D.×250 mm Mobile phase : acetonitrile:water=70 : 30 Flow rate : 1.0 ml/min Temperature : 30°C Detection : RI	Sample 1.Arabinose 2.Galactose 3.Maltitol

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Poster presented at PITTCON 2005

Title :
Sugar-D: A Distinctly Improved Stationary Phase for Saccharide Separation

Pittcon2005_Sugar-D.pdf (52.3KB)

Ordering information

Product name	Size	Code No.	End-fitting
COSMOSIL^® Sugar-D Guard Column	4.6 mm I.D.× 10 mm	05394-81	Waters type
COSMOSIL^® Sugar-D Guard Column	10.0 mm I.D.× 20 mm	05696-31
COSMOSIL^® Sugar-D Packed column	2.0 mm I.D.× 150 mm	05688-41
	2.0 mm I.D.× 250 mm	05689-31
	3.0 mm I.D.× 150 mm	05690-91
	3.0 mm I.D.× 250 mm	05691-81
	4.6 mm I.D.× 150 mm	05395-71
	4.6 mm I.D.× 250 mm	05397-51
	10.0mm I.D.× 250 mm	05692-71
	20.0mm I.D.× 250 mm	05693-61

Other sizes may be available. Please enquire!