HOME > Reagents > WEST-Easy Western Blotting Detection System
|
|
Western Blotting Detection System
WEST-Easy Western Blotting Detection System
|
|
WEST-Easy Western Blotting Detection System is a light emitting non-radioactive
method for detection of immobilized specific antigens in chemiluminescent
Western blots through horseradish peroxidase (HRP) labeled antibodies.
Conventional Western blotting detection systems typically consist of two
bottles, one for chemiluminescent substrate solution and one for enhancer
solution. The WEST-Easy system combines both chemiluminescent substrate
solution and enhancer solution in one spray bottle. There is no mixing
of solutions required.
|
|
Features |
Convenient : Just spray, no need to mix substrate solution and enhancer solution
High sensitivity : Up to 1-2 pg of antigen on membrane can be detected
Long signal : Strong light emission lasts up to 2 hours
|
|
Schematic of WEST-Easy
|
|
| Sensitivity and Signal Duration Test |
|
 |
|
| Technical Information |
WEST-Easy Procedure |
|
Ordering information
|
Product name
|
Storage |
Product No.
|
PKG Size |
WEST-Easy Western Blotting Detection System
|
Refrigerator |
NU16031 |
100ml |
|
|
|
|
|
Chemi-Lumi One L (Chemiluminescent Substrate Kit)
|
|
|
| Chemi-Lumi One L is a nonradioactive light emitting system based on
chemiluminescent substrate for the detection of horseradish peroxidase (HRP)
labeled antibodies. Chemi-Lumi One L is designed for reaching maximal strength
quickly and lasting luminescence for at least 120 minutes. |
|
| Features |
Longer light emission : strong light emission for more than 120 minutes
Highly sensitive : intense signal with low background
Fast : rapid substrate processing of blot
|
Principle |
|
 |
|
The peroxidase(POD)-catalyzed oxidization of luminol by hidrogenperoxiide produces 3-aminophthalate dianion, a blue fluorescent compound, providing a very sensitive method for detection of proteins after Western blotting |
|
.
|
| Comoarison |
 |
Protein Markers (Biotinylated) for Western Blotting (Product No.: 02886-44)
Lane 1: 1:50 dilution
Lane 2: 1:100 dilution
Lane 3: 1:200 dilution
Lane 4: 1:400 dilution |
 |
Step 1
Block nonspecific sites with Blocking One
(Product No.:03953-95).
Step 2
Bind to horseradish peroxidase with Streptavidin Biotin Complex Peroxidase Kit
(Product No.:30462-30)
Step 3
Detect with Chemi-Lumi One L or competitors' chemiluminescence detection kits |
|
Ordering information |
Product name
|
Storage |
Product No.
|
PKG Size |
Chemi-Lumi One L
|
Refrigerator |
07880-70 |
1kit |
|
|
|
HOME > Reagents > WEST-Easy Western Blotting Detection System |
| (C)2004-2008 Nacalai Tesque, Inc. E-mail: info.intl@nacalai.co.jp |