BCIP-NBT Solution Kit

Features

  • Easy to use: BCIP and NBT solution in one bottle
  • Fast: half reaction time of conventional kits
  • DNase and RNase free
Product Images

BCIP-NBT Solution Kit is highly sensitive alkaline phosphatase (ALP) staining kit for blotting. The buffer contains ALP catalyst for the same detection sensitivity of conventional methods. Only half the reaction time of conventional methods.

Principle

principle

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Composition

Content Components Quantity Number Storage
Staining Stock Solution 5-Bromo-4-chloro-3-indoryl Phosphate (BCIP) / Nitroblue Tetrazolium (NBT) 2 ml 1 bottle - 20°C
Buffer Solution Tris-HCl Buffer Solution, Magnesium Chloride 200 ml 1 bottle 4°C

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Application

Western blotting :

After blotting antigen conjugates to the antibody-ALP in Western blotting, the targeted protein is detected with high sensitivity. In the following experiment 60 ng protein was detected.

Application

 Sample human serum 
 Sample amount ① prestained markers ② 5 µg ③ 1.7 µg ④ 0.55 µg ⑤ 0.2 µg ⑥ 60 ng ⑦ 20 ng 
 Primary antibody  Anti-human IgG (Goat)
 Secondary antibody  Anti-goat IgG (Rabbit) ALP conjugated
 Electrophoresis  12.5% SDS-PAGE (35 mA, 40 minutes)
 Membrane  PVDF membrane
Exposure time   30 minutes

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Protocols

Not Available

References

  1. Y Tsuneoka et al.: Journal of Comparative Neurology 521.7 (2013)
  2. A Apilux et al.: Lab on a Chip 13.1 (2013)
  3. Y Oji et al.: International journal of cancer125.2 (2009)
  4. H Ishii et al.: Histopathology 38.2 (2001)
  5. Langer, P. R. &Ward, D. C. in Developmental Biology Using Purified Genes,ICN-UCLA Symposium on Molecular and Cellular Biology,ed.Brown,D.D.(Academic,NewYork), 23, pp.647-658. (1983)
  6. Fukumaki, Y., Ghosh, P. K., Benz, E. J., Jr., Reddy, V. B., Lebowitz, P., Forget, B. G. & Weissman, S. M. Cell 28, 585-593 (1982)
  7. Karin, M., Andersen, R. D. & Herscheman, H. R. Eur. J. Biochem. 118, 527-531. (1981)
  8. Hsu, S. M., Raine, L. & Fanger, H. J. Histochem. Cytochem. 29, 577-580. (1981)
  9. Wigler, M., Sweet, R.,Sim, G.K., Wold, B., Pellicer,A., Lacy, R., Maniatis, T., Silverstein, S. & Axel, R. Cell 16, 777-785. (1979)
  10. Wilson, J. T., Wilson, L. B., deRiel, J. K., Villa-Komaroff, L., Efstratiadis, A., Forget, B. G. &Weissman, S. M. Nucleic Acids Res. 5, 563-581. (1978)

Downloads

BCIP-NBT Solution Kitpdf(PDF 1.43 MB)

Ordering information

Product name Grade Storage Code No. Quantily Online
Catalog 
BCIP-NBT Solution Kit
for Alkaline Phosphatase Stain		 SP Freezer 03937-60 1 kit e-Nacalai

(Storage)
A = Cool and dark, RT = Room temperature, F = Freezer, R= Refrigerator,