Conventional C18 columns often do not offer enough retention for hydrophilic compounds. This technical note shows analysis of monoamines, including catecholamine and serotonin, by LC-MS/MS using COSMOSIL PBr.

Monoamine LC-MS/MS analysis

The standard chloroform/methanol extraction method for monoamines in mouse brain tissue resulted in peak distortion caused by impurities. For this experiment, a spin column was used for sample preparation. After preparation, the amounts of dopamine, noradrenaline and serotonin were quantified. 

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