COSMOSIL Index
COSMOSIL 3.0 mm I.D. Columns
- Use the same conventional HPLC systems
- Reduce half solvent consumption
- Increase sensitivity up to two times
Comparison with 4.6 mm I.D. Column
For more information about scale down, please see Inner Diameter of Column(scale down and scale up) (PDF 344 KB)
| Condition | |||
|---|---|---|---|
| Column | COSMOSIL 5C18-AR-II | Sample |
|
| Mobile Phase | Methanol : Water = 70 : 30 | ||
| Temperature | 30°C | ||
| Detection | UV 254 nm 0.5 AUFS | Injection Vol. | 1.5 l |
Theoretical Plate Number
A 3.0 mm I.D. column can be used with any conventional HPLC system without modification. However, theoretical plate number (N) decreases by 10-20% compared with a 4.6 mm I.D. column using the same packing material. The reason for this decrease is the increase in the ratio of system dead volume to the volume of column, and not caused by the column itself. Therefore, it is recommended to use a 3.0 mm I.D. column when the resolution is high enough. It is an excellent choice to minimize sample volume and reduce mobile phase and waste disposal cost.
Figure 1 shows the relation between theoretical plate number and flow rate of a 3 mm I.D. column using a conventional HPLC system and a micro HPLC system, respectively. The 3 mm I.D. column has a low theoretical plate number when connected to a conventional HPLC system with big dead volume. On the other hand, as shown in Figure 2, a 4.6 mm I.D. column is no influenced as much by the difference in dead volume on different HPLC systems.
For more information on respective column.
Ordering Information
Analytical Column (Particle size : 5 µm)
| Product Name | Size | Product No. | Online Catalog |
|---|---|---|---|
| COSMOSIL 3C18-EB Packed Column | 3.0 mm I.D. x 150 mm | 09814-51 |  |
| 3.0 mm I.D. x 250 mm | 09827-91 | ||
| COSMOSIL 5C18-MS-II Packed Column | 3.0 mm I.D. x 150 mm | 34245-31 | |
| 3.0 mm I.D. x 250 mm | 34254-11 | ||
| COSMOSIL 5C18-AR-II Packed Column | 3.0 mm I.D. x 150 mm | 38028-61 | |
| 3.0 mm I.D. x 250 mm | 38029-51 | ||
| COSMOSIL 5C18-PAQ Packed Column | 3.0 mm I.D. x 150 mm | 05797-11 | |
| 3.0 mm I.D. x 250 mm | 05798-01 | ||
| COSMOSIL Cholester Packed Column | 3.0 mm I.D. x 150 mm | 05973-91 | |
| 3.0 mm I.D. x 250 mm | 05974-81 | ||
| COSMOSIL πNAP Packed Column | 3.0 mm I.D. x 150 mm | 08080-91 | |
| 3.0 mm I.D. x 250 mm | 08081-81 | ||
| COSMOSIL Sugar-D Packed Column | 3.0 mm I.D. x 150 mm | 05690-91 | |
| 3.0 mm I.D. x 250 mm | 05691-81 |