COSMOSIL PBB-R strongly retains iodine atom which has a large dispersion force, than bromine atom. So it can separate halogen exchange reaction products that are difficult to analyze with C18 column.
Sample courtesy of Dr. H. Yamamoto, RIKEN
Condition | ||
---|---|---|
Column Size | 4.6 mm I.D. x 150 mm | |
Flow Rate | 5PBB-R Methanol : Water = 100 : 0 5C18-MS-II Methanol : Water = 90 : 10 |
|
Temperature | 30°C | |
Detection | UV 254 nm | |
Sample | Halogen exchange reaction products |
C18 column can not separate Triton X-100 mixturem, because (-OCH2CH2-) group has little hydrophobicity. However, COSMOSIL PBB-R can separate them because it distinguishes difference in the dispersion force, which depends on its molecular weight.
Condition | ||
---|---|---|
Column Size | 4.6 mm I.D. x 150 mm | |
Flow Rate | 5PBB-R Methanol : Water = 100 : 0 5C18-MS-II Methanol : Water = 80 : 20 |
|
Temperature | 30°C | |
Detection | UV 254 nm | |
Sample | Triton X-100 (5C18-MS-II 50 µg) (5PBB-R 100 µg) |