COSMOSIL C18-MS-II
Features
- Multi-purpose C18 Column
- High reproducibility
- A wide range of applications
Product Information
Column Specifications
Separation Property
The COSMOSIL 5C18-MS-II is a well-balanced column with better basic performance such as sharper peaks of basic compounds and chelating compounds, large hydrophobic interaction, low analytical pressure, and high theoretical plate number. The COSMOSIL 5C18-MS-II is first-choice column for reversed phase chromatography.
Separation Property
| Condition | |
|---|---|
| Column Size | 4.6 mm I.D. x 150 mm |
| Mobile Phase | Methanol/ H2O = 60/40 |
| Flow Rate | 1.0 ml/min |
| Temperature | 30°C |
| Detection | UV254nm |
| Sample |
|
Table. Comparison of hydrophobic interaction, analytical pressure, and theoretical plate number
| Column | Hydrophobic Interaction α (Toluene/Benzene) |
Pressure (MPa) |
Theoretical Plate Number (Toluene) |
|---|---|---|---|
| COSMOSIL 5C18-MS-II | 1.96 | 8.3 | 14300 |
| A Company C18 | 1.99 | 13.0 | 16800 |
| B Company C18 | 1.94 | 8.0 | 14000 |
| C Company C18 | 1.69 | 11.2 | 5600 |
| D Company C18 | 1.84 | 10.5 | 14200 |
Analysis of Basic Compounds and Metal Coordination Compounds
The COSMOSIL 5C18-MS-II column, taking advantage of a new end-capping treatment, can replace the original COSMOSIL C18 (ODS) column. A new end-capping treatment with polar groups for "shield effect" has significantly improved peak shape for basic compounds. Ultra pure silica gel with low trace-metal content is used for COSMOSIL columns; thus the columns provide excellent peak shapes for chelating compounds.
Basic Compounds
| Condition | |
|---|---|
| Column Size | 4.6 mm I.D. x 150 mm |
| Mobile Phase | Methanol/ 20mmol/l Phosphate buffer(pH7) = 20/80 |
| Flow Rate | 1.0 ml/min |
| Temperature | 30°C |
| Detection | UV254nm |
| Sample |
|
Metal Coordination Compounds
| Condition | |
|---|---|
| Column Size | 4.6 mm I.D. x 150 mm |
| Mobile Phase | Acetonitrile / 20mmol/l Phosphoric Acid = 5/95 |
| Flow Rate | 1.0 ml/min |
| Temperature | 30°C |
| Detection | UV240nm |
| Sample | Oxine-copper |
High Reproducibility
The strict quality control system of Nacalai Tesque supports the customers with an individual “Inspection Report” which accompanies each and every COSMOSIL and COSMOGEL Packed Column (except guard columns) and an additional “Certificate of Analysis”for the COSMOSIL 5C18-MS-II (4.6 mm I.D. x 150 mm and 4.6 mm I.D. x 250 mm). For more informations, please click here.
Applications
Parasympatholytic Agents
| Condition | |
|---|---|
| Column Size | 4.6 mm I.D. x 150 mm |
| Mobile Phase | Methanol/ 20mmol/l Phosphate buffer(pH7) = 30/70 |
| Flow Rate | 1.0 ml/min |
| Temperature | 30°C |
| Detection | UV210nm, 0.2AUFS |
| Sample |
|
Analgesic Antipyretic Drugs
| Condition | |
|---|---|
| Column Size | 4.6 mm I.D. x 150 mm |
| Mobile Phase | Methanol/ 20mmol/l Phosphate buffer(pH7) = 30/70 |
| Flow Rate | 1.0 ml/min |
| Temperature | 30°C |
| Detection | UV254nm, 0.16AUFS |
| Sample |
|
See more COSMOSIL ApplicationsUsage information
| Column | 5 µm C18-MS-II | |||||||
|---|---|---|---|---|---|---|---|---|
| I.D. (mm) | 1.0 | 2.0 | 3.0 | 4.6 | 6.0 | 10.0 | 20.0 | 28.0 |
| Washing method | 1. Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. For example, if your mobile phase was 50:50 methanol/20 mmol/l phosphate buffer, wash with 50:50 methanol/water. 2. (If you used a buffer, always do step 1 first!) Remove adsorbed compounds and fix unstable baselines by washing with methanol and/or THF. |
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| Storage conditions | Short-term (up to a week): Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. For example, if your mobile phase was 50:50 methanol/20 mmol/l phosphate buffer, wash with 50:50 methanol/water. Long-term: Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. Then, replace the solvent with 70% methanol or acetonitrile : 30% water. In either case, tightly plug the column, and store in a cool and dry place. |
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| Usable pH range | pH 2-10 (pH 2-7.5 recommended) |
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| Max. pressure | 20MPa | 15MPa | ||||||
| Temperature range | The maximum usable temperature is 60°C. However. for regular use, please use at a constant temperature between 20°C and 50°C. | |||||||
| Usable solvents | Any solvent that will not dissolve the silica gel (such as alkaline solutions) or cleave the stationary phase (such as very acidic solutions) is usable. Note: For solvents with high viscosity, please watch the system pressure and keep it below 20 MPa. Also, please wash the column after using acidic mobile phases or solvents with high freezing points. |
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| Other instructions | - Buffer concentration is usually sufficient at 0.005 - 0.02 mol/L. - Always filter mobile phases using a 0.5 um or finer filter before use. - Reproducibility may worsen when using a mobile phase that is more than 90% water. |
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Downloads
Brochure
COSMOSIL 5C18-MS-II
(659 KB)
References
Reference list
Ordering Information
Analytical Column (Particle Size : 5 µm)
COSMOSIL 5C18-MS-II
| Product Name | Size | Product No. | Price |
|---|---|---|---|
| COSMOSIL 5C18-MS-II Guard Column/Guard Cartridge | |||
| 4.6 mm I.D. x 10 mm | 38014-31 |  |
|
| 4.6 mm I.D. x 10 mm Cartridge | 38015-89 | ||
| 10 mm I.D. x 20 mm | 38016-11 | ||
| 20 mm I.D. x 20 mm | 05790-81 | ||
| 20 mm I.D. x 50 mm | 34371-71 | ||
| 28 mm I.D. x 50 mm | 34347-01 | ||
| COSMOSIL 5C18-MS-II Packed Column | |||
| 1.0 mm I.D. x 50 mm | 02824-31 | |
|
| 1.0 mm I.D. x 150 mm | 02896-01 | ||
| 2.0 mm I.D. x 30 mm | 05876-71 | ||
| 2.0 mm I.D. x 50 mm | 04355-21 | ||
| 2.0 mm I.D. x 100 mm | 05597-31 | ||
| 2.0 mm I.D. x 150 mm | 38025-91 | ||
| 2.0 mm I.D. x 250 mm | 05761-61 | ||
| 3.0mmI.D. x 100mm | 05458-51 | ||
| 3.0 mm I.D. x 150 mm | 34245-31 | ||
| 3.0 mm I.D. x 250 mm | 34254-11 | ||
| 4.6 mm I.D. x 30 mm | 34341-61 | ||
| 4.6 mm I.D. x 50 mm | 38017-01 | ||
| 4.6 mm I.D. x 100 mm | 38018-91 | ||
| 4.6 mm I.D. x 150 mm | 38019-81 | ||
| 4.6 mm I.D. x 250 mm | 38020-41 | ||
| 6.0 mm I.D. x 150 mm | 38021-31 | ||
| 6.0 mm I.D. x 250 mm | 38022-21 | ||
| 10 mm I.D. x 50 mm | 05789-21 | ||
| 10 mm I.D. x 150 mm | 34355-91 | ||
| 10 mm I.D. x 250 mm | 38023-11 | ||
| 20 mm I.D. x 150 mm | 05091-41 | ||
| 20 mm I.D. x 250 mm | 38024-01 | ||
| 28 mm I.D. x 250 mm | 05760-71 |
4.6 mm I.D. x 10 mm Cartrige contains 3 PKG. Cartridge Holder (Product number: 38009-79) is required.
Preparative Column (Particle Size : 15 µm)
COSMOSIL 15C18-MS-II
| Product Name | Size | Product No. | Price |
|---|---|---|---|
| COSMOSIL 15C18-MS-II Guard Column | |||
| 28 mm l.D. x 50 mm | 05885-51 | |
|
| 50 mm l.D. x 50 mm | 34527-41 | ||
| COSMOSIL 15C18-MS-II Packed Column | |||
| 28 mm l.D. x 250 mm | 34525-61 | |
|
| 50 mm l.D. x 250 mm | 05886-41 | ||
| 50 mm l.D. x 500 mm | 34531-71 |
For more information on 15C18-MS-II
Fast LC Column (Particle Size : 3 µm)
COSMOSIL 3C18-MS-II
| Product Name | Size | Product No. | |
|---|---|---|---|
| COSMOSIL 3C18-MS-II Packed Column | |||
| 2.0 mm l.D. x 50 mm | 05514-01 | |
|
| 4.6 mm l.D. x 10 mm | 38065-71 | ||
| 4.6 mm l.D. x 50 mm | 38066-61 | ||
| 4.6 mm l.D. x 100 mm | 38067-51 |
Ultra-High Performance Columns (Particle Size : 2.5 µm)
COSMOSIL 2.5C18-MS-II
| Product Name | Size | Product No. | |
|---|---|---|---|
| COSMOSIL 2.5C18-MS-II Packed Column |
|||
| 2.0 mm l.D. x 50 mm | 08994-31 | |
|
| 2.0 mm l.D. x 75 mm | 08995-21 | ||
| 2.0 mm l.D. x 100 mm | 08996-11 | ||
| 3.0 mm l.D. x 50 mm | 08997-01 | ||
| 3.0 mm l.D. x 75 mm | 08998-91 | ||
| 3.0 mm l.D. x 100 mm | 08999-81 |
For more information on 2.5C18-MS-II
For more information on COSMOSIL 3.0 mm I.D. columns
Please refer to Inner Diameter of Column(scale down and scale up) (PDF 344 KB)
(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze
