Products

0.01%-Polyethyleneimine Coating Solution

0.01% Polyethyleneimine Coating Solution is a ready-to-use coating reagent for primary neuronal cell culture. This product can be used for coating various cell culture labware.

Features

  • Applicable for primary neuronal cell culture
  • Suitable for various types of cell culture labware
  • Store at room temperature

Application 1

This product's performance was assessed using primary cultured neurons derived from the mouse fetal brain cortex.

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It was confirmed that the cytotoxicity of this product to primary cultured neurons significantly decreased with a minimum of one wash. (The calculation is based on the cell viability after 5 washes, set at 100%.)

Application 2

The optimal number of washes was determined using primary cultured neurons derived from the mouse fetal brain cortex or hippocampus.

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It was confirmed that the cytotoxicity of this product to primary cultured neurons significantly decreased with a minimum of one wash. (The calculation is based on the cell viability after 5 washes, set at 100%.

Application 3

We compared the performance of coating with this product and poly-L-lysine using primary cultured neurons derived from the mouse fetal brain hippocampus.

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Our product exhibits the same adhesiveness performance as poly-L-lysine coating.

Application 4

Primary cultured neurons from the mouse fetal brain hippocampus were successfully maintained and cultured on various cell culture labware coated with this product for an extended period.

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In various types of culture labware coated with this product, we were able to maintain primary cultured neurons derived from the mouse fetal hippocampus for three weeks. Furthermore, conducting immunofluorescence staining on these cells did not result in any detachment, allowing for the detection of MAP2.

Application 5

Primary cultured neurons derived from the rat fetal brain cortex or hippocampus were cultured on cell culture labware coated with this product.

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It was confirmed that by using this product for coating, it was possible to achieve adherent culture of primary cultured neurons derived from the rat fetal brain cortex or hippocampus.

Ordering Information

Product Name Grade Storage Product Number PKG Size Price
0.01%-Polyethyleneimine Coating Solution For TC SP RT(2years) 21958-14 100 mL e-Nacalai