AscleStem® Cardiomyocyte Differentiation Medium
AscleStem® Cardiomyocyte Differentiation Medium (#13166-05), Supplement A for C.D. Medium (#15345-24), and Supplement B for C.D. Medium (#15346-14) are serum-free medium and serum-free supplements for inducing differentiation of human pluripotent stem cells into cardiomyocytes. By replacing cytokines with small-molecule compounds, this protocol is expected to reduce costs, and it can be applied to both the monolayer culture method (adherent culture) and the embryoid body formation method (suspension culture).
Features
- Differentiates human pluripotent stem cells into cardiomyocytes
- Lower cost with cytokine-free protocol
- Compatible with monolayer and embryoid body methods
Product description
Example protocol for human iPSC-derived cardiomyocyte differentiation using this medium/supplement
Cell morphology and immunostaining results after differentiation using two culture methods
Human iPSC cell lines were differentiated into cardiomyocytes using two culture methods: the monolayer culture method and the embryoid body formation method. Expression of the cardiomyocyte marker cTnT was confirmed by immunostaining using purified cells.
Response of cardiomyocytes differentiated by two culture methods to isoproterenol
The responsiveness of cardiomyocytes differentiated from human iPS cells by two culture methods (monolayer culture and embryoid body formation) to isoproterenol was evaluated using the SI8000 Cell Motion Imaging System (Sony). Similar results were obtained with both methods.
Downloads
Brochures
- AscleStem® Cardiomyocyte Differentiation Medium
(PDF 1.5 MB)
Ordering information
| Product name | Grade | Storage | Catalog number | PKG size | Price |
|---|---|---|---|---|---|
| AscleStem® Cardiomyocyte Differentiation Medium | SP | Refrigerate | 13166-05 | 500 mL |  |
| Supplement A for C.D. Medium | – | Freezer | 15345-24 | 11 mL | |
| Supplement B for C.D. Medium | – | Freezer | 15346-14 | 5 mL | |
AscleStem is a registered trademark of Nacalai Tesque, Inc.
