AscleStem® hPSC Maintenance Supplement
AscleStem® hPSC Maintenance Supplement (#21979-44), is a feeder-free culture supplement for maintaining undifferentiated human pluripotent stem cells (hPSCs). It can be easily prepared by simply adding it to DMEM/Ham’s F-12 medium. This product is based on the composition of a clinical grade undifferentiated maintenance medium developed through collaborative research with Osaka University. Please refer to the provided references for more information.
Features
- Simply add it into DMEM/Ham's F-12 (#11581-15)
- Available to reduce the cost
- Applicable for the weekend skip protocol
- Medium components are disclosed (Concentration is confidential)
Protocol
After thawed this product (stored in a frozen state) in a refrigerator, add the entire contents to 500 ml of DMEM/Ham’s F-12. Please use it after thorough stirring.
Application
Cell morphology and alkaline phosphatase activity
The cell morphology of the 253G1 hiPSCs was confirmed after 2 weeks of culture with this product (prototype). Alkaline phosphatase staining was also performed to confirm that undifferentiated ability was not affected.
Reference
Hua Y, Yoshimochi K, Li J, Takekita K, Shimotsuma M, Li L, et al. Development and evaluation of a novel xeno-free culture medium for human-induced pluripotent stem cells. Stem Cell Res Ther. 2022;13:223.
DOI: 10.1186/s13287-022-02879-z https://pubmed.ncbi.nlm.nih.gov/35658933/
Ordering Information
| Product Name | Grade | Storage | Catalog Number | PKG Size | Price |
|---|---|---|---|---|---|
| AscleStem® hPSC Maintenance Supplement | SP | Freezer | 21979-44 | 32 mL |  |
AscleStem is a registered trademark of Nacalai Tesque, Inc.
