For Xeno-free Hematopoietic Stem Cell CultureAscleStem® HSC Medium
This product is a basal medium designed for hematopoietic stem cell culture. The addition of six cytokine supplements to
this medium enables the culture of hematopoietic stem cells (CD45dim CD34+).
Features
- Enables expansion culture of hematopoietic stem cells
- Xeno-Free
- Experience in CiRA protocols for iPSC generation from human PBMCs
Product Information
Evaluation of Hematopoietic Stem Cell Expansion
Human peripheral blood mononuclear cells (PBMCs) were cultured for five days in this medium supplemented with cytokines. After cell counting, expression of CD34 and CD45 was analyzed by flow cytometry. The results demonstrate that this medium efficiently supports the expansion of hematopoietic stem cells (CD45dim CD34+).
Culture Protocol Overview
Cell culture was performed with reference to a protocol of CiRA Foundation.
https://www.cira-foundation.or.jp/j/assets/file/publications/EpisomalVector_iPS_protocol.pdf
Application
Application of This Medium to iPS Cell Derivation
Human peripheral blood mononuclear cells (PBMCs) were cultured in this medium supplemented with six cytokines, followed by generation of iPS cells based on the RNA reprogramming method1.
References
Application Reference
1. Nakagawa M, Nogi M, Doi H, et al. MDM4 enables efficient human iPS cell generation from PBMCs using synthetic RNAs. Sci Rep. 2025; 15: 30620.
DOI: https://doi.org/10.1038/s41598-025-16446-y
Background Reference
Human IPS Cells—Reprogramming Human Peripheral Blood-Derived Mononuclear Cells (PBMCs) Using Synthetic RNA
https://www.cira.kyoto-u.ac.jp/e/research/protocol.html
Ordering Information
| Product Name | Grade | Storage | Catalog Number | PKG Size | Price |
|---|---|---|---|---|---|
| AscleStem® HSC Medium | SP | Freezer | 22562-25 | 500 mL |  |
AscleStem is a registered trademark of Nacalai Tesque, Inc.
