hiPSCs-derived neural progenitor cells (day 17 after differentiation induction) were seeded at 500,000 cells/cm² and induced to differentiate into neurons by adding GlutaMAX and other inducers to our medium/our supplement or Company A's medium / their supplement. Immunostaining was performed to confirm the expression of MAP2, a marker of mature neurons. We confirmed that hiPSC-derived neurons cultured in AscleStem^® Neuronal Medium and AscleStem^® Neuronal Supplement(50x) were comparable in hiPSC-derived neurons cultured in Thermo's system.

Using our supplement or Company A's supplement, human iPS cells were induced to differentiate into motor neurons. Immunostaining was performed to confirm the induction of motor neurons by choline acetyltransferase (ChAT) and the elongation of nerve axons by β III tubulin.