Bullet CBB Stain Lite
Bullet CBB Stain Lite is a one-step, 15 minutes complete Coomassie Brilliant Blue staining solution. Compared to our previous
product, it reduces staining time and steps, allowing for a clear staining image in a shorter period. Additionally, since there is
no need for a destaining step, the staining image can be observed during the staining process.
Features
- In one step, complete staining in 15 minutes
No need for gel pre-staining washing
Clear staining image without the need for a destaining step
- Free from strong irritants like acetic acid and methanol
Applications
Staining image within the staining solution
Stained protein samples after electrophoresis using this product. User can visualize staining image within a tray containing the staining solution using this product.
Conditions
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Sample | Protein extract from E. coli,dissolve in sample buffer (for SDS-PAGE, 2x, 2-ME) (#30566-22) |
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SDS-PAGE |
Bullet PAGE Plus Precast Gel, 5-20%, 17wells(#21794-54)400V(constant voltage), for 10 minutes |
CBB stain |
Bullet CBB Stain Lite(#21964)at room temperature for 15 minutes |
Comparison
Comparison of various CBB staining solutions
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Bullet CBB Stain Lite (#21964) |
CBB Stain One Super (#11642) |
CBB Stain One (#04543) |
Rapid Stain CBB Kit (#30035) |
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Dye | CBB G-250 | CBB R-250 | CBB G-250 | CBB R-250 |
Package contents |
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Pretreatment | No need | Washing (3 times x 5 mins.) |
Washing (3 times x 5 mins.) |
Adjustment (Mixing two liquids) |
Staining time | 15 mins.* | 30 mins. | 60 mins. | 20 mins. |
Destaining | No need* | Over 1 hr. | ||
Detection sensitivity | Several tens of ng (BSA) |
* For gel drying/preservation, stain and destain for 1 hour each.
Example of staining
Staining image after staining procedure (without destaining)
This product allows user to visualize staining image with a low background without destaining step.
Staining image after destaining step
This product allows for the visualization of staining image after 15 minutes of staining without a destaining step. However, by extending both staining and destaining steps to 60 minutes each, a clearer staining image can be achieved.
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Sample | Diluted Protein Markers (10x)(#29458-24) into ① 3x, ② 1x, ③ 1/3x, and ④ 1/10x, then added 2 µL. |
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SDS-PAGE |
Extra PAGE One Precast Gel, 5-20%, 13wells(#13063-74) 400 V(constant voltage), for 30 mins. |