Bullet PAGE Plus Precast Gel
Bullet PAGE Plus is a precast gel for high-speed electrophoresis. Despite being able to complete electrophoresis in approximately 10 minutes, it allows the use of Laemmli buffer for electrophoresis and sample preparation, resulting in sharp bands. Additionally, a wide range of gel concentrations is available in the lineup
Features
- Only 10 minutes with 400 V
- Wide range of gel concentrations
- Works well in conventional Laemmli running buffer and sample buffer
- Surfactant-free, so can be used for nucleic acid analysis＊
＊Please use 25 mM Tris and 192 mM glycine buffer for nucleic acid electrophoresis
Comparison
Performance Comparison
This product demonstrates separation ability and band sharpness comparable to conventional products, despite a short running time of about 10 minutes.
横スクロール可能Scroll
| Product name | Bullet PAGE Plus | Extra PAGE One | Company A | Company B |
|---|---|---|---|---|
| Gel concentration | 5 - 20% | 5 - 20% | 4 - 20% | 4 - 12% |
| Product number | 21794-54 | 13064-64 | － | － |
| Running time | 10 m 07 s | 35 m 12 s | 29 m 35 s | 48 m 32 s |
| Electrophoresis image |  |
 |
 |
 |
(Condition)
- Sample:
- Protein marker (10x) (#29458-24), 3 µL
- Gel staining:
- CBB Stain One (Ready To Use)（#04543）
- Running condition:
- As per the recommended conditions for each product. (Bullet PAGE Plus 400 V ,Extra PAGE One 300 V）
Comparison of transfer efficiency
This product shows a transfer efficiency equivalent to Company A products
横スクロール可能Scroll
| Transfer solution | Bullet Semi-dry Transfer One (#15353-01) | |
|---|---|---|
| Transfer condition | 25 V, 10 min | |
| Gel | Bullet PAGE Plus 5 - 20% |
Company A 4 - 20% |
| Image of after transfer |  |
 |
(Conditions)
- Sample:
- ①Protein Ladder One Plus, Triple-color for SDS-PAGE (#19593-25)
Dilute Protein Marker (10x) (29458-24) to achieve ② 3x, ③ 1x, and ④ 1/3x, 2 µL of each - Staining:
- Stain the PVDF membrane with CBB Stain One (Ready To Use)（#04543）
Application
Western blotting: Detection of proteins by different sizes
Three proteins of different sizes were detected by western blotting after running by using this product. A wide range of proteins of different molecular weights, from low (Histone H3) to high (EGFR), could be detected, indicating that the product is applicable to western blotting.
横スクロール可能Scroll
| Protein detection | EGFR（175 kDa） | β-Actin（42 kDa） | Histone H3（17 kDa） |
|---|---|---|---|
| Blotting image |  |
 |
 |
(Condition)
- Sample:
- HeLa cell suspension (10 µg to 2-fold dilution series)
- SDS-PAGE:
- Bullet PAGE Plus Precast Gel, 5-20%, 17wells (#21794-54) 400 V
- Transfer:
- Bullet Semi-dry Transfer One (#15353-01) 25 V 10 min. Use PVDF (bore size; 0.45µm)
- Blotting:
- Bullet Blocking One for Western Blotting (#13779) for 5 mins at RT
- Primary antibody:
-
[EGFR] EGF Receptor (D38B1) XP Rabbit mAb (CST #4267) 2,000x dilution
Signal Enhancer HIKARI for Western Blotting and ELISA
Dilute by Signal Enhancer HIKARI for Western Blotting and ELISA (#02270-81) A solution for 60 mins at RT[β-Actin] β-Actin antibody(C4) (Santa Cruz #sc-47778) 1,000 x dilution
Dilute by Bullet ImmunoReaction Buffer (#18439-85) for 30 mins at RT[Histone H3] Histone H3 (D1H2) XP Rabbit mAb (CST #4499) 2,000x dilution
Dilute by Bullet Blocking One for Western Blotting (#13779) for 60 mins at RT - Secondary Antibody：
-
[EGFR]
Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP](Pre-adsorbed) (Novus #NB7187) 50,000x dilution
Signal Enhancer HIKARI for Western Blotting and ELISA
Dilute by Signal Enhancer HIKARI for Western Blotting and ELISA (#02270-81) B solution for 60 mins at RT[β-Actin] β -Actin HRP-labeled Anti-Mouse IgG (self-prepared) 5,000x dilution
Diluted by Bullet ImmunoReaction Buffer (#18439-85) for 30 minutes at RT[Histone H3] Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] (Pre-adsorbed) (Novus #NB7187) 30,000x dilution
Diluted by Bullet Blocking One for Western Blotting (#13779) for 60 minutes at RT - Detection:
-
ChemiDoc Touch MP 3 × 3 binning (Bio-Rad Laboratories) (AUTO mode)[EGFR]
[β-Actin]
[Histone H3]Chemi-Lumi One Super (#02230)
Chemi-Lumi One L (#07880)
Chemi-Lumi One Super (#02230)
Product line-up
The following types of gradient and homogeneous gels are available.
The following types of gradient and homogeneous gels are available.
横スクロール可能Scroll
| Gel | Gradient gel | |||||
|---|---|---|---|---|---|---|
| Gel concentration | 5 - 10% | 5 - 15% | 5 - 20% | 7.5 - 15% | 10 - 20% | |
| Product number | 13 wells | 21789-34 | 21791-84 | 21793-64 | 21795-44 | 21797-24 |
| 17 wells | 21790-94 | 21792-74 | 21794-54 | 21796-34 | 21798-14 | |
|
Images of protein |
 |
 |
 |
 |
 |
|
| Images of nucleic acids (PAGE) |  |
 |
 |
 |
 |
|
横スクロール可能Scroll
| Gel | Homogeneous gel | ||||
|---|---|---|---|---|---|
| Gel concentration | 7.5% | 10% | 12.5% | 15% | |
| Product number | 13 wells | 21799-04 | 21801-44 | 21807-84 | 21853-74 |
| 17 wells | 21800-54 | 21806-94 | 21811-14 |
21854-64 | |
|
Images of protein |
 |
 |
 |
 |
|
| Images of nucleic acids (PAGE) |  |
 |
 |
 |
|
How to use
If use a power supply with maximum 300 V, please refer to the following running times. The running time is the time it takes for the BPB in the sample buffer to migrate to 5 mm above the lower edge of the gel. The running time varies slightly depending on the temperature of the running buffer and the gel concentration.
横スクロール可能 Scroll
| Voltage | Running time | |
|---|---|---|
| Protein analysis (SDS-PAGE) | Nucleic acids analysis (PAGE) | |
| 400 V | About 10 min | About 12 min |
| 300 V | About 16 min | About 18 min |
For protein analysis (SDS-PAGE), use 25 mM Tris, 192 mM glycine and 0.1% SDS buffer. For nucleic acid analysis (PAGE), use 25 mM Tris, 192 mM glycine buffer. It is recommended that no more than half the well volume (20 µL for 13 wells and 14 µL for 17 wells) of adding samples.
Specifications
横スクロール可能Scroll
| Plate | W100 mm × H80 mm × T3.2 mm (made of glass) |
|---|---|
| Size of gel | W80 mm × H60 mm × T1.0 mm |
| Number of well (well capacity) | 13 wells (40 µL), 17 wells (28 µL) |
<Notice>
- Use tank which can fit plates of size W100 mm × H80 mm × T3.2 mm.
Recommended model: WEP-MN Vertical Electrophoresis Tank (Gellex International #WEP-MN) - Check the suitability of the electrophoresis system (e.g. plate size) and the performance of the power supply (e.g. power range) before use.
Rerated products
横スクロール可能Scroll
| Manufacture name | Gellex International |
|---|---|
| Product name | WEP-MN vertical high-speed electrophoresis bath |
| Product number | WEP-MN |
| Dimensions | W143 × D84 × H140 (mm) |
| Buffer quantity | 550 mL |
* The electrophoresis bath is supplied with an adaptor (100 mm x 80 mm) for Bullet PAGE Plus.
Ordering information
横スクロール可能Scroll
| Product name | Storage | Product number | PKG size | ||||
|---|---|---|---|---|---|---|---|
| Bullet PAGE Plus Precast Gel, 5-10%, 13wells | Refrigerate | 21789-34 | 10 sheets | ||||
| Bullet PAGE Plus Precast Gel, 5-10%, 17wells | Refrigerate | 21790-94 | 10 sheets | ||||
| Bullet PAGE Plus Precast Gel, 5-15%, 13wells | Refrigerate | 21791-84 | 10 sheets | ||||
| Bullet PAGE Plus Precast Gel, 5-15%, 17wells | Refrigerate | 21792-74 | 10 sheets | ||||
| Bullet PAGE Plus Precast Gel, 5-20%, 13wells | Refrigerate | 21793-64 | 10 sheets | ||||
| Bullet PAGE Plus Precast Gel, 5-20%, 17wells | Refrigerate | 21794-54 | 10 sheets | ||||
| Bullet PAGE Plus Precast Gel, 7.5-15%, 13wells | Refrigerate | 21795-44 | 10 sheets | ||||
| Bullet PAGE Plus Precast Gel, 7.5-15%, 17wells | Refrigerate | 21796-34 | 10 sheets | ||||
| Bullet PAGE Plus Precast Gel, 10-20%, 13wells | Refrigerate | 21797-24 | 10 sheets | ||||
| Bullet PAGE Plus Precast Gel, 10-20%, 17wells | Refrigerate | 21798-14 | 10 sheets | ||||
| Bullet PAGE Plus Precast Gel, 7.5%, 13wells | Refrigerate | 21799-04 | 10 sheets | ||||
| Bullet PAGE Plus Precast Gel, 7.5%, 17wells | Refrigerate | 21800-54 | 10 sheets | ||||
| Bullet PAGE Plus Precast Gel, 10%, 13wells | Refrigerate | 21801-44 | 10 sheets | ||||
| Bullet PAGE Plus Precast Gel, 10%, 17wells | Refrigerate | 21806-94 | 10 sheets | ||||
| Bullet PAGE Plus Precast Gel, 12.5%, 13wells | Refrigerate | 21807-84 | 10 sheets | ||||
| Bullet PAGE Plus Precast Gel, 12.5%, 17wells | Refrigerate | 21811-14 | 10 sheets | ||||
| Bullet PAGE Plus Precast Gel, 15%, 13wells | Refrigerate | 21853-74 | 10 sheets | ||||
| Bullet PAGE Plus Precast Gel, 15%, 17wells | Refrigerate | 21854-64 | 10 sheets | ||||
