Cell Lysis Buffer
Cell Lysis Buffer is designed for protein extraction, similar to RIPA Buffer, and is available in two forms: a ready-to-use type and a 10x concentrated type. The 10x concentrated type is packaged separately with SDS solution, which may adversely affect immunoprecipitation.
Features
- Available for protein extraction in the same way as RIPA Buffer
- Does not include OPE/NPE (e.g., NP-40), substances regulated under REACH
- Available in two forms
Lineup
Available in two forms as below.
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| Product name | Cell Lysis Buffer | Cell Lysis Buffer (10x) |
|---|---|---|
| Product type | 1x (ready to use) | 10x |
| Protease inhibitor cocktail |
Not included | Included |
| SDS | Included | Not included (10x SDS solution is packed separately) |
| Storage | Refrigerator | Freezer |
Comparison
Protein extraction by using Cell Lysis Buffer or other company’s RIPA Buffer
CBB staining
Protein was extracted from rat kidney by using this product or Company A’s RIPA Buffer, then subjected to electrophoresis, and CBB staining. The result shows protein extraction efficiency of this product is equal to that of Company A’s RIPA Buffer.
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| Conditions | |
|---|---|
| Sample |
① Protein extracted by using this product (15 μg) |
| SDS-PAGE | Bullet PAGE Plus Precast Gel, 5-15%, 13wells (#21791-84) 400 V |
| Staining | Bullet CBB Stain Lite (#21964) |
Western blotting
Protein was extracted from mouse liver by using this product or Company A’s RIPA Buffer, then subjected to western blotting, and CBB stained. The results show that this product is applicable to western blotting, similar to Company A’s RIPA Buffer.
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| Conditions | |
|---|---|
| Sample | ① Protein extracted by using this product ② Protein extracted by using Company A’s RIPA Buffer [β-Actin] 7 µg [PCNA] 7 µg [EGFR] 28 µg |
| SDS-PAGE | Bullet PAGE Plus Precast Gel, 5-15%, 13wells (#21791-84) 400 V |
| Transfer | Bullet Semi-dry Transfer One (#15353-01) 25 V |
| Blocking | Bullet Blocking One for Western Blotting (#13779) |
| Primary antibody |
[β-Actin] β-Actin antibody (C4) (Santa Cruz #sc-47778) 1,000x diluted |
| Secondary antibody |
[β-Actin] Anti-Mouse IgG HRP (#21860) 20,000x diluted |
| Detection | Chemi-Lumi One Super (#02230) |
Application 1
Phosphorylated protein detection by using extracts from Cell Lysis Buffer
Protein was extracted from Protein Kinase C (PKC)-activated HEK293 cells by using this product, and ERK and pERK were detected by Western blotting.
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| Conditions | |
|---|---|
| Sample | ① Protein extracted from the HEK293 cell (Control) ② Protein extracted from PKC-activated HEK293 cells |
| SDS-PAGE | Bullet PAGE Plus Precast Gel, 5-15%, 13wells (#21791-84) 400 V |
| Transfer | Bullet Semi-dry Transfer One (#15353-01) 25 V |
| Blocking | Bullet Blocking One for Western Blotting (#13779) |
| Primary antibody |
[ERK] ERK antibody (Santa Cruz #sc-514302) 2,000x diluted |
| Secondary antibody |
[ERK] Anti-Mouse IgG HRP (#21860) 20,000x diluted |
| Detection | Chemi-Lumi One Super (#02230) |
Application 2
Protein extraction by using Cell Lysis Buffer (10x)
CBB staining
Protein was extracted from various mouse organs by using this product with SDS, subject to electrophoresis and CBB staining.
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| Conditions | |
|---|---|
| Sample |
①,⑦ Protein Ladder One Plus, Triple-color for SDS-PAGE (#19593-25) |
| SDS-PAGE | Bullet PAGE Plus Precast Gel, 5-15%, 13wells (#21791-84) 400 V |
| Stain | Bullet CBB Stain Lite (#21964) |
immunoprecipitation
Protein was extracted from GFP-expressing HEK293 cells using this product without SDS and immunoprecipitated using rat anti-GFP antibody-conjugated agarose beads. GFP was detected by Western blotting of the samples before and after immunoprecipitation. GFP in the samples before and after immunoprecipitation was detected by Western blotting.
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| Conditions | |
|---|---|
| Sample |
① Sample before immunoprecipitation (3 μL) ② Sample after immunoprecipitation (3 μL) |
| SDS-PAGE | Bullet PAGE Plus Precast Gel, 5-15%, 13wells (#21791-84) 400 V |
| Transfer | Bullet Semi-dry Transfer One (#15353-01) 25 V |
| Blocking | Bullet Blocking One for Western Blotting (#13779) |
| Primary antibody | Anti-GFP (Mouse IgG1-κ) (#04363) 5,000x diluted |
| Secondary antibody | Anti-Mouse IgG HRP (#21860) 20,000x diluted |
| Detection | Chemi-Lumi One Super (#02230) |
Ordering information
| Product name | Grade | Storage | Product number | PKG size |
Price |
|---|---|---|---|---|---|
| Cell Lysis Buffer | SP (for biochemical research) |
Refrigerator | 22352-04 | 100 mL |  |
| Cell Lysis Buffer(10x) | Freezer | 22353-81 | 1 set | |
