Reagents

Reference

Peroutka, R.J., N. Elshourbagy, T.L. Piech, and T.R. Butt, Enhanced protein expression in mammalian cells using engineered SUMO fusions: Secreted phospholipase A2. Prot. Sci., 2008. 17:1586-1595.

Liu, L., J. Spurrier, T.R. Butt, and J.E. Strickler,?Enhanced protein expression in the baculovirus/insect cell system using engineered SUMO fusions.?Prot. Exp. Purif., 2008. doi:10.1016/j.pep.2008.07.010.

Hughes SR, Dowd PF, Hector RE, Panavas T, Sterner DE, Qureshi N, Bischoff KM, Bang SS, Mertens JA, Johnson ET, Li XL, Jackson JS, Caughey RJ, Riedmuller SB, Bartolett S, Liu S, Rich JO, Farrelly PJ, Butt TR, Labaer J, Cotta MA.?(2008). Lycotoxin-1 insecticidal peptide optimized by amino acid scanning mutagenesis and expressed as a coproduct in an ethanologenic Saccharomyces cerevisiae strain. J PeptSci. 9:1039-50.?

Panavas, T, Sanders C, F and Butt, T. R. (2008) SUMO fusion technology for enhanced protein production in prokaryotic and eukaryotic expression systems In Methods in Molecular Biology: SUMO Protocols, Ullrich, H, ed. Humana Press, in press

Nicholson, B., Leach, Craig A, Goldenberg, Seth J, Francis, Dana M, Kodrasov, Matthew P, Tian, Xufan, Shanks, John, Sterner, David E, Bernal, Alejandro, Mattern, Michael R, Wilkinson, Keith D, and Butt, Tauseef R. (2008). "Characterization of Ubiquitin and Ubiquitin-like Protein Isopeptidase Activities." Protein Science, 17: 1035-1043

Nicholson, B., Marblestone, J.G., Butt, T. R., and Mattern, M. R.(2007) "Deubiquitinating Enzymes as Novel Anticancer Targets." Future Oncology, 3: 191-199

Hughes, S.R., Dowd, P.F., Hector, R.E., Riedmuller, S.B., Bartolett, S., Mertens, J.A., Qureshi, N., Liu, S., Bischoff, K. M., Li, X-L., Jackson, Jr, J.S., Sterner, D., Panavas, T., Rich, J., Farrelly, P.J., Butt, T.R., Cotta, M.J. (2007) "High-Throughput Multiplex Library Construction of Synthetic Mutagenized Insecticidal Peptides Using a Fully Automated Plasmid-Based Functional Proteomic Robotic Workcell with an Improved Vacuum Block." J Assoc Lab Automation J. Assoc. Lab. Autom. 12(4):2002-2012

Guzzo CM, Yang D.? “Systematic analysis of fusion and affinity tags using human aspartyl-tRNA synthetase expressed in E.coli.”Protein Expr Purif., 2007 54(1): 166-175.

Arnold, JJ, Bernal, A, Uche, U, Sterner, DE, Butt, T. R, Cameron, CE, and Mattern, MR. 2006. "SUMO isopeptidase assay based on poliovirus RNA polymerase activity." Analytical Biochem, 350: 214-221

Marblestone JG, Edavettal SC, Lim Y, Lim P, Zuo X, Butt TR. "?Comparison of SUMO fusion technology with traditional gene fusion systems: Enhanced expression and solubility with SUMO."?Protein Science, 2006 15:182-9.

Hall J. "Applying Fusion Protein Technology to E. coli."?BioPharm International, 2006 February.
Hall J, Mattern MR, Butt TR. "Mining the Ubiqitin Pathway."?The Scientist, 2005 19(23):42-43.

Butt TR, Edavettal SC, Hall JP, Mattern MR. "?SUMO fusion technology for difficult-to-express proteins."?Protein Expr Purif., 2005 43(1):1-9.

Zuo X, Li S, Hall J, Mattern MR, Tran H, Shoo J, Tan R, Weiss SR, Butt TR. "Enhanced expression and purification of membrane proteins by SUMO fusion in Escherichia coli."?J Struct Funct Gen., 2005 6:103-11.

Hall J. "Production of Difficult-to-Express Proteins."?Genetic Engineering News, 2005 25(9):52-53.

Zuo X, Mattern MR, Tan R, Li S, Hall J, Sterner DE, Shoo J, Tran H, Lim P, Sarafianos SG, Kazi L, Navas-Martin S, Weiss SR, Butt TR. "Expression and purification of SARS coronavirus proteins using SUMO-fusions."?Protein Expr Purif.,?2005 42(1):100-10.

Malakhov MP, Mattern MR, Malakhov OA, Drinker M, Weeks SD, Butt TR.?"SUMO fusions and SUMO-specific protease for efficient expression and purification of proteins."?J Struct Funct Gen, 2004 5:75-86.

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