Rapid Running Buffer Solution(20x) for SDS-PAGE
A running buffer for hand-made SDS-PAGE gels, which is able to dramatically shorten protein migration time. Please note that the product is designed for hand-made gels. Any commercially available precast gels should be evaluated beforehand.
Features
- Time-saving: Approximately 25-minute protein migration time with mini-gel on 250 V
- Simple: Replace Laemmli running buffer with Rapid Running Buffer Solution
- Gradient gel-like separation pattern: Wider separation range
- High transfer efficiency: Lower acrylamide concentration gel recommended
- Applicable to Wide Range Gel*
*Wide Range Gel: Hand-made gels casted with Wide Range Gel Preparation Buffer, Catalog No. 07831-94, which offer gradient gel-like separation pattern -- the gradient in pore size offers wide range of molecular weights separation in a single run -- and also have tensile strength character.
Product Information
This running buffer offers shorter protein migration time than Laemmli running buffer. No significant difference of buffer temperature increase with Laemmli running buffer shows.
Comparison of protein migration time and buffer temperature with Laemmli running buffer
| Gel consentration | Running buffer |
|---|---|
| 8% | Rapid Running Buffer |
| 12% | Laemmli Running Buffer (0.025 mol/l tris, 0.192 mo/l L-glysin and 0.1% SDS) |
Comparison of separation pattern on the same acrylamide percentage gel
| Gel concentration | 8% | 8% |
| Running buffer | Rapid Running Buffer | Laemmli running buffer |
| Transfer time required | 23 minutes | |
|
Separation pattern |
|
 |
Tips
Lower acrylamide concentration required
To have a similar separation pattern running with Rapid Running Buffer, 4% lower acrylamide concentration should be used as the below table.
| Laemmli method | |
|---|---|
| Gel concentration | Running buffer |
| 12% | Laemmli running buffer |
| Saving-time protocol | |
| Gel concentration | Running buffer |
| 8% | Rapid Running Buffer |
Separation pattern
| Gel |
|
|---|---|
| Running buffer |
|
| Sample | Protein ladder manufactured by Nacalai, #29458-24 |
| Voltage | 250 V |
| Gel staining | CBB Stain One（#04543） |
Laemmli gel run with Rapid Running Buffer
| Running buffer | Rapid Running Buffer | |||
| Gel | Laemmli gel | |||
| Gel concentration | 6% | 8% | 10% | 4-10% |
| Separation pattern |  |
 |
 |
 |
Laemmli gel run with Laemmli running buffer
| Running buffer | Laemmli running buffer | ||||
| Gel | Laemmli gel | ||||
| Gel concentration | 6% | 8% | 10% | 12% | 15% |
| Separation pattern |  |
 |
 |
 |
 |
Wide Range Gel run with Rapid Running Buffer
To separate high molecular weight area, Wide Range Gel will be helpful.
| Running buffer | Rapid Running Buffer | |||
| Gel | WIDE RANGE Gel | |||
| Gel concentration | 4% | 6% | 8% | 10% |
| Separation pattern |  |
 |
 |
 |
Application
2-D electrophoresis
Rapid Running Buffer is also applicable to 2-D electrophoresis.
| Sample | HL-60 cell extract |
|---|---|
| 1-D | Immobiline pH 4-7 |
| 2-D | 10% Laemmli mini gel |
| Running buffer | Rapid Running Buffer |
| Voltage | 250 V、25minutes |
| Gel staining | Sil-best Stain One manufactured by Nacalai, #06865-81 |
Immubiline® is a trademark by Cytiva.
Transfer efficiency
Because of lower acrylamide concentration, protein transfer efficiency from a gel to a membrane will improve.
| Gel concentration | 8% | 12% | 8% | 12% |
| Running buffer | Rapid Running Buffer | Laemmli running buffer | Rapid Running Buffer | Laemmli running buffer |
| Transfer time required | 10 minutes | 30 minutes | ||
|
Membrane staining image |
|
 |
|
|
| Gel | Laemmli mini gel |
|---|---|
| Sample | 1 lane Protein Ladder One, Triple-color, #09547-74, 5 μl 2-4 lane Protein Marker, #29458-24, 3x, 1x, 1/3x 2μl each |
| Voltage | 250 V |
| Transfer buffer/Voltage | Semi-dry Transfer Buffer, #30650-31/10 V |
| Menbrane staining | CBB Stain One（#04543） |
Note
- Rapid Running Buffer is designed for hand-made gels, but not for Native-PAGE gels.
Any commercially available precast gels should be evaluated beforehand. - Pre-stained marker bands might appear slightly bigger molecular weight than they actual have due to dye conjugation.
- Actual protein migration time and buffer temperature increased may depend on a gel composition and its size, amount of running buffer, type of chamber, etc.
Video
Rapid Running Buffer Solution(20x) for SDS-PAGE
Download
Rapid Running Buffer Solution(20x) for SDS-PAGE 
(PDF 2.4 MB)
Ordering information
| Product Name | Grade | Storage | Catalog Number | PKG Size | e-Nacalai |
|---|---|---|---|---|---|
|
Rapid Running Buffer Solution (20x) for SDS-PAGE |
SP |
Room temperature |
12981-74 | 250 mL |  |
Related products
| Product Name | Catalog Number | PKG Size |
|---|---|---|
| ■ Gel casting reagents | ||
| 40(w/v)%-Acrylamide/Bis Mixed Solution(29:1) | 06119-45 | 500 ml |
| 40(w/v)%-Acrylamide/Bis Mixed Solution(37.5:1) | 06121-95 | 500 ml |
| 30(w/v)%-Acrylamide/Bis Mixed Solution(29:1) | 06141-35 | 500 ml |
| 30(w/v)%-Acrylamide/Bis Mixed Solution(37.5:1) | 06144-05 | 500 ml |
| 10%-SDS Solution | 30562-04 | 100 ml |
| N,N,N',N'-Tetramethylethylenediamine | 33401-72 | 25 g |
| 10(w/v)%-Ammonium Peroxodisulfate Solution | 02634-34 | 10 ml |
| WIDE RANGE Gel Preparation Buffer(4x) for PAGE | 07831-94 | 250 ml |
| ■ Running buffer | ||
| Running Buffer Solution(10x) for SDS-PAGE, Tris-Glycine | 30329-61 | 1 L |
| ■ Sample preparation reagents | ||
| Sample Buffer Solution with 2-ME(2x) for SDS-PAGE | 30567-12 | 25 ml |
| Sample Buffer Solution without 2-ME(2x) for SDS-PAGE | 30567-12 | 25 ml |
| Sample Buffer Solution with Reducing Reagent(6x) for SDS-PAGE | 09499-14 | 5 ml |
| Sample Buffer Solution without Reducing Reagent(6x) for SDS-PAGE | 09500-64 | 5 ml |
| ■ Gel staining products | ||
| Dispotray-S Outer size:150 mm x 105 mm x 19 mm Bottom face size: 122 mm x 80 mm | 16526-82 | 25 pieces |
| Dispotray-M Outer size:200 mm×140 mm×25 mm Bottom face size: 155 mm×105 mm | 16551-84 | 25 pieces |
| Rapid Stain CBB Kit | 30035-14 | 1 set |
| CBB Stain One(Ready To Use) | 04543-51 | 1 L |
| Sil-Best Stain One | 06865-81 | 1 set |
