Reduced-serum Medium for Gene TransfectionModified-MEM for Transfection

Modified-MEM for Transfection is an MEM-based medium designed for gene transfection.

Features

Medium optimized for transfection
Supports low-serum culture
Medium composition disclosed

Product Description

Transfection using this product or competitor media

Cells were transfected with a plasmid vector using this product or Company A’s reduced-serum medium, which is commonly used for transfection, together with various transfection reagents. This product showed performance comparable to that of Company A’s reduced-serum medium.

Transfection of HEK293 cells with the pmCherry-N1 vector

Transfection of CHO-K1 cells with the pAcGFP1-N1 vector

siRNA transfection using this product or competitor media

siRNA was introduced into HEK293 cells using Company A’s transfection reagent together with either this product or Company A's reduced-serum medium, and the results were evaluated by Western blotting.

Application

Transfection using this product and DailyFect Transfection Reagent

Using this product and DailyFect Transfection Reagent, 1 μg of the pAcGFP1-N1 vector was introduced into two types of adherent cells. Transfection efficiency was evaluated 48 hours after transfection by microscopy and flow cytometry.

Cell proliferation rate and morphology under reduced-serum conditions

HEK293 cells were cultured using this product containing 5% FBS. Cell proliferation rate and morphology comparable to those observed with DMEM containing 10% FBS were obtained. These results confirmed that cells can be cultured under low-serum conditions using this product.