Products

Signal Enhancer HIKARI for Western Blotting and ELISA

Features

Product Image

  • Enhances antigen-antibody reaction: yields several- to several 10-fold increase in signal intensity
  • Easily removes background: improves the specificity of your antibodies
  • Works with any substrate: enhances both chemiluminescence and colorimetric detection
  • Works with any membrane: enhances signal from nitrocellulose and PVDF membrane
  • Ready-to-use reagent: just dilutes your antibodies with Signal Enhancer HIKARI

Product Information

Dilute your antibodies with Signal Enhancer HIKARI instead of conventional diluents such as PBS or TBS before performing your next Western blot detection protocol and witness a remarkable increase in the ability to detect the protein of interest and to eliminate undesired background.
Signal Enhancer HIKARI was developed to resolve the problems of low sensitivity and high background often encountered during procedures such as Western blotting and ELISA.

Kit Contents

Solution A for Primary Antibody
Solution B for Secondary Antibody

Applications

Detection enhancement of Rac1 with treatment of HIKARI in Western blotting

Treatment with Signal Enhancer HIKARI resulted in clear signal, whereas dilution with conventional PBS-T resulted in no signal.

Application_1_Rac1

Primary Ab: Anti-Rac1 (1: 1,000)
Secondary Ab: Anti-Mouse IgG-HRP (1: 2,500) Sample: Rat cortical primary neuron
Detection: ECL

Data courtesy of Dr. K. Ishizuka, Department of Neuroscience, Johns Hopkins University School of Medicine

Detection enhancement of pJNK with treatment of HIKARI in Western blotting

Treatment with Signal Enhancer HIKARI resulted in clear signal enhancement and background suppression compared with the conventional method using TBS-T.

Application_2_pJNK

Primary Ab: Anti-pJNK (1: 1,000)
Secondary Ab: Anti-rabbit IgG - HRP (1: 5,000)
Sample: Mouse Embryonic Fibroblast (MEF)
Detection: SuperSignal West Pico

Data courtesy of Dr. S. Matsuzawa,
Signal Transduction, NCI Cancer Center,
Burnham Institute for Medical Research

Protocols

Signal Enhancer HIKARI Procedure for Westen Blotting and ELISZApdf(PDF 86 KB)

References

  1. Dawn E. Barnes et al. Cytoskeleton 73, 117-130(2016)
  2. J Tan et al.: Biotechnology journal 10.5 (2015)
  3. M Koike et al.: The Journal of Veterinary Medical Science 77.9 (2015)
  4. Janice G.L. Tan et al. Biotechnology Journal 10, 790-800(2015)
  5. M. Suzuki et al. Clinical & Experimental Immunology 182, 154-161(2015)
  6. Hideki Ueno et al. Biochemical and Biophysical Research Communications 468, 4-11(2015)
  7. K Shintani-Ishida et al.: Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease 1842.5 (2014)
  8. R Stavniiichuk et al.: Experimental neurology 230.1 (2011)
  9. T Takenouchi et al.: Journal of neurochemistry117.4 (2011)
  10. Feng-Ming Yang et al. FEBS 276, 425-436(2009)
  11. Jian-Bin Wang et al. The Journal of Cell Science 122(12), 2024-2033(2009)
  12. Chunwei Huang et al. Reproductive Toxicology 27, 103-110(2009)
  13. Sawako Yamashiro et al. The Journal of Cell Science 121(Pt 23), 3867-3877(2008)

Downloads

Signal Enhancer HIKARI for Western Blottingpdf (PDF 906 KB)

Ordering Information

Product Name Kit Contents Grade Storage Product No. PKG Size Price
Signal Enhancer HIKARI
for Western Blotting and ELISA		 Solution A
for primary antibodies
Solution B
for secondary antibodies		 SP 4°C 02267-41 1set
(50ml each)		 e-Nacalai
02270-81 1set
(250ml each)
Signal Enhancer HIKARI
for Western Blotting and ELISA Solution A		 02272-74 250ml
Signal Enhancer HIKARI
for Western Blotting and ELISA Solution B		 02297-64 250ml

(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze