Products

Blocking One

Features

Product Image

  • Fast: Short blocking time
  • Wide range: Contains both high molecular weight compounds and bovine serum proteins
  • Easy to use: Simple storage in refrigerator even after the bottle is opened

Product Information

Blocking is indispensable in immunoassays in order to block non-specific binding reactions. Blocking One contains both high molecular weight compounds and bovine serum protein. Blocking One is superior to conventional blocking solutions. The preservative in Blocking One does not affect the enzyme activity of peroxidase (POD) or alkaline phosphatase (ALP). Only simple refrigerator storage is necessary, even after opening the bottle.

Comparison of blocking reaction times

Application

The relationship between reaction time and the effect of blocking in microplate assay. In comparison with other products, Blocking One shows by far the fastest dinamics.

Since the antigen reaction is omitted in this experiment, when staining occurs, it is caused by non-specific adsorption. The figure shows negative data

Applications

Linearity in quantitative protein analysis

App

This product does not contain preservative which affects the enzyme activity of Peroxidase (POD) or Alkaline Phosphatase (ALP). Using common enzyme (POD, ALP) gives good result with high linearity.

Detection of Mouse IgG(H+L) by Western Blotting

App

Incubation time for blocking : 20 min.
Sample M: Biotinylated Protein Marker (100 dilution, 5 μl)
1 : Mouse Serum (2.5 μl)
2 : Mouse Serum (5.0 μl)
3 : Mouse Serum (10 μl)
Mouse IgG (H+L) in Mouse Serum detected with Biotinylated anti-Mouse
IgG (H+L)

Very little background occurs, indicating exceptional blocking performance.

Prevention of non-specific antibody binding in immunohistochemical application

App

Histological staining of normal organ tissue slices had been performed based on the following protocol, and the efficiency of different blocking agents wrere compared (20 minutes blocking time). The dots stained brown show Fibronectin existence. The key for successful observation is the contrast between the stained dots and the background, Blocking One gives the lowest background.

Protocols

Blocking One Seriespdf (PDF 100 KB)

References

  1. H Kubota et al.: Food chemistry 206 (2016): 78-84
  2. K Umezu et al.: Journal of Reproduction and Development 0 (2016)
  3. Y Kobayashi et al.: Allergology International (2016)
  4. Y Ishida et al.: Biochemical and biophysical research communications471.4 (2016)
  5. H Furumoto et al.: Toxicology and applied pharmacology 296 (2016): 1-9
  6. M Isoda et al.: Neuroscience research (2016)
  7. K Yeom et al.: International endodontic journal 49.3 (2016): 271-278
  8. Y Dulla et al.: Neurochemical Research (2016): 1-11
  9. Y Kotani et al.: Scientific reports 5 (2015)
  10. Y Uchino et al.: Biological and Pharmaceutical Bulletin 38.6 (2015): 926-929
  11. Y Sonoda et al.: Neuropathology (2015)
  12. K Kono et al.: Biologicals 43.2 (2015): 146-149
  13. C Sakuma et al.: Biochemical and biophysical research communications 458.1 (2015)
  14. J Terashima et al.: Drug metabolism and pharmacokinetics 30.6 (2015): 434-440
  15. A Fukagawa et al.: Cancer medicine 4.1 (2015): 125-135
  16. T Okinaga et al.: Journal of Interferon & Cytokine Research 35.6 (2015)
  17. T Torii et al.: Biochemical and biophysical research communications 465.3 (2015)
  18. H Yoshitomi et al.: BMC complementary and alternative medicine 15.1 (2015): 1
  19. Q Meng et al.: International journal of nanomedicine 10 (2015): 2313
  20. M Rahman et al.: Biochemistry and Biophysics Reports 4 (2015): 234-242

Downloads

Blocking One Seriespdf (PDF 1.2 MB)

Ordering Information

Product Name Grade Storage Product No. PKG Size Price
Blocking One SP R 03953-95 500 ml e-Nacalai

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(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze