Phosphatase Inhibitor Cocktail is a mixture of several inhibitors to protect valuable proteins from dephosphorylation. The product preserves phosphorylated proteins existing in small quantity in cells and tissues. There is an EDTA free product which is useful for extraction or purification of protein.

Comparison data

Figure 1. The detection at phosphorylated proteins in HeLa cells with p-Thr antibody.

Figure 2. The inhibition effect of phosphatase assayed using fluorescence labeled p-Tyr peptide substrate.

Figure 3. The inhibition effect of phasphatase assayed using p-Nitrophenylphosphoric Acid.

Composition

The product (100 times concentrated stock solution) contains:

Components	Phosphatase Inhibitor Cocktail (EDTA free) (Product No.: 07575-51)	Phosphatase Inhibitor Cocktail (Product No.: 07574-61)
Sodium Orthovanadate (V)	o	o
Disodium Molybdate(VI) Dihydrate	o	o
Sodium (+)-Tartrate Dihydrate	o	o
Imidazole	o	o
Sodium Fluoride	o	o
β-Glycerophosphoric Acid Disodium Salt	o	o
tetra-Sodium Ethylenediaminetetraacetate	---	o

Reference

1. Yang, JH. et al. The Journal of Biological Chemistry (2010)
2. Selamat, W. et al. Neuroscience Letters 450(2), 163-166 (2009)
3. Saito, T. et al. Biochemical and Biophysical Research Communications 357(2), 371-376 (2007)
4. Murakami, Y. et al. J. Biochem., 141, 401-410 (2007)
5. Takenaga, M. et al. J. Cell Sci., 120, 2078-2090 (2007)

Not Availalbe

• Phosphatase Inhibitor Cocktail (EDTA free)(PDF 100 KB)
• Phosphatase Inhibitor Cocktail(PDF 100 KB)

Phosphatase Inhibitor Cocktail (EDTA free)(PDF 558 KB)