Compositions

Compositions of each product are available on online catalog, "e-Nacalai Search Version".

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Cell Culture Medium(PDF 6 MB)

Ordering information

Product Name	Storage	Product No.	PKG Size	Price
DMEM(1.0g/l Glucose) with L-Gln and Sodium Pyruvate,liquid	R	08456-65	500 ml
		08456-36	10 bottles x 500 ml
DMEM (1.0g/lGlucose) with Sodium Pyruvate, without L-Gln and Phenol Red, liquid	R	08490-05	500 ml
DMEM (4.5g/l Glucose) with L-Gln and HEPES, without Sodium Pyruvate, liquid	R	08457-55	500 ml
DMEM (4.5g/l Glucose) with L-Gln and Sodium Pyruvate, liquid	R	08458-45	500 ml
		08458-16	10 bottles x 500 ml
DMEM (4.5g/l Glucose) with L-Gln, without Sodium Pyruvate, liquid	R	08459-35	500 ml
		08459-64	10 bottles x 500 ml
DMEM (4.5g/l Glucose) without L-Gln and Sodium Pyruvate, liquid	R	08488-55	500 ml
DMEM (4.5g/l Glucose) without L-Gln, Sodium Pyruvate and Phenol Red, liquid	R	08489-45	500 ml
DMEM(4.5g/l Glucose) with L-Gln, Sodium Pyruvate and 1.5g/l Sodium Hydrogen Carbonate, liquid	R	16971-55	500 ml
DMEM(4.5g/l Glucose) Calcium free without L-Gln and Sodium Pyruvate, liquid	R	16972-45	500 ml
DMEM (4.5g/l Glucose) with Sodium Pyruvate, without L-Gln, liquid	R	11584-85	500 ml
DMEM (4.5g/l Glucose) with HEPES, without L-Gln and Sodium Pyruvate, liquid	R	11585-75	500 ml
DMEM (No Glucose) with L-Gln, without Sodium Pyruvate, liquid	R	09891-25	500 ml
DMEM/Ham's F-12 with L-Gln, Sodium Pyruvate and HEPES, liquid	R	08460-95	500 ml
DMEM/Ham's F-12 with L-Gln, Sodium Pyruvate and HEPES, without Phenol Red, liquid	R	05177-15	500 ml
DMEM/Ham's F-12 with L-Gln and Sodium Pyruvate, without HEPES, liquid	R	11581-15	500 ml
DMEM/Ham's F-12 with L-Gln and Sodium Pyruvate, without HEPES and Phenol Red, liquid	R	11582-05	500 ml
DMEM/Ham's F-12 with Sodium Pyruvate and HEPES, without L-Gln, liquid	R	11583-95	500 ml
DMEM/Ham's F-12 (No Glucose) with L-Gln and Sodium Pyruvate, liquid	R	09893-05	500 ml
IMDM with L-Gln and HEPES, liquid (Iscove's Modified Dulbecco's Medium)	R	11506-05	500 ml
Ham's F-12 with L-Gln, liquid	R	17458-65	500 ml
G-MEM with L‐Gln, liquid	R	12965-65	500 ml
MEM with Earle's Salts and L-Gln, liquid	R	21442-25	500 ml
MEM with Earle's Salts, L-Gln and Non-Essential Amino Acids, liquid	R	21443-15	500 ml
MEM (No Glucose) with Earle's Salts, L-Gln and Non-Essential Amino Acids, liquid	R	09848-05	500 ml
α-MEM with L-Gln, Ribonucleosides and Deoxyribonucleosides, liquid	R	21444-05	500 ml
α-MEM with L-Gln, without Ribonucleosides and Deoxyribonucleosides, liquid	R	21445-95	500 ml
RPMI 1640 with L-Gln and HEPES, liquid	R	30263-95	500 ml
RPMI 1640 with L-Gln, liquid	R	30264-85	500 ml
		30264-56	10 bottles x 500 ml
RPMI 1640 with L-Gln, without Phenol Red, liquid	R	06261-65	500 ml
RPMI 1640 with L-Gln, liquid	R	05176-25	500 ml
RPMI 1640 (No Glucose) with L-Gln, liquid	R	09892-15	500 ml
RPMI 1640(4.5g/l Glucose) with L-Gln, Sodium Pyruvate and HEPES	R	16970-65	500 ml
Ham's F-12 with L-Gln, liquid	R	17458-65	500 ml
MEM with Earle's Salts and L-Gln, liquid	R	21442-25	500 ml
MEM with Earle's Salts, L-Gln and Non-Essential Amino Acids, liquid	R	21443-15	500 ml

(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze

• Sterilized by 0.2 µm membrane filter, tested for bacteria, fungus, mycoplasma and endotoxin

Ordering information

Product Name	Storage	Product No.	PKG Size	Price
D-PBS(+) Preparation Reagent (Ca,Mg Solution) (100x)	RT	02492-94	30 ml
D-PBS(-) without Ca and Mg, liquid	RT	14249-95	500 ml
		14249-24	10 bottles x 500 ml
D-PBS(-) without Ca and Mg, liquid(10x)	R	11482-15	500 ml
D-PBS(-) without Ca and Mg, Powder	RT	07269-84	100 g
Phosphate Buffered Saline without KCl(pH 7.2)	RT	11480-35	500 ml
Phosphate Buffered Saline without KCl(10x)(pH 7.2)	RT	11481-25	500 ml
Phosphate Buffered Saline without KCl(pH 7.4)	RT	13397-85	500 ml
HBSS(+) with Ca, Mg and Phenol Red, liquid	RT	17459-55	500 ml
HBSS(+) with Ca, Mg, without Phenol Red, liquid	RT	09735-75	500 ml
HBSS(-) without Ca and Mg, with Phenol Red, liquid	RT	17460-15	500 ml
HBSS(-) without Ca, Mg and Phenol Red, liquid	RT	17461-05	500 ml

(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze

• Sterilized by 0.2 µm membrane filter, tested for bacteria, fungus, mycoplasma and endotoxin

MEM Non-Essential Amino Acids Solution (100x)

Composition: Non-Essential Amino Acids 10 mM each

Non-Essential Amino Acids	(mg/ml)
L-Alanine	890
L-Asparagine,H2O	1,500
L-Asparatic Acid	1,330
L-Glutamic Acid	1,470
Glycine	750
L-Proline	1,150
L-Serine	1,050

100mM-Sodium Pyruvate Solution (100x)

Pyruvic Acid Solution Culture Test:

Ordering information

Product Name	Storage	Product No.	PKG Size	Price
L-Alanyl-L-glutamine	R	01102-82	25 g
200mmol/l L‐Alanyl‐L‐glutamine Solution(100x)	F	04260-64	100 ml
200mM‐L‐Glutamine Stock Solution	F	16948-04	100 ml
0.1mol/l-Calcium Chloride Solution	RT	16973-64	20 ml
45(w/v)%-D-(+)-Glucose Solution	RT	16974-54	100 ml
1mol/l-HEPES Buffer Solution	R	17557-94	100 ml
MEM Non-Essential Amino Acids Solution(100x)	R	06344-14	20 ml
		06344-56	100 ml
100mM-Sodium Pyruvate Solution(100x)	R	06977-34	100 ml
apo-Transferrin from Human	R	34401-84	100 mg
		34401-55	500 mg

(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze

Animal Free Cell Media Additives

• Wide variety of products (recombinant protein, amino Acids, vitamins, others)

Product Type	Number of Items	Test Items
Recombinant proteins	8	Endotoxin test and other
Amino Acids	28	Endotoxin test（max 0.1 EU/mg） Cell culture test Purity
Vitamins	8
Others	4

Ordering information

Product Name	Storage	Purity	Product No.	PKG Size	Price
Recombinant proteins
Albumin, Human, Recombinant expressed in Rice, Animal-Free	R	96%	12880-81	1 g	inquiry
			12880-94	5 g
			12880-52	25 g
[Test item]Endotoxin: max. 1 EU/mg, Solubility in water: Clear to almost clear, Loss on drying: 15%, pH 6.5-7.5
Insulin, Human, Recombinant expressed in Yeast, Animal-Free	F	96%	12878-86	50 mg
			12878-44	250 mg
[Test item] Endotoxin: max. 10 EU/mg, Potency (calculated on dried substance): min. 27.5 units/mg, Loss on drying: max. 10%, Zinc (calculated on dried substance): 0.3-1.0%
Transferrin, Human, Recombinant expressed in Rice, Animal-Free	R	95%	12879-34	100 mg
			12879-05	500 mg
			12879-21	1 g
[Test item] Endotoxin: max 1.EU/mg, Water: max. 10%, Iron(Fe): max. 0.1
Proteins
apo-Transferrin from Human	R	≧96.0%(EA)	34401-84	100 mg
			34401-55	500 mg
Albumin, Bovine Serum, Low Endotoxin, pH5.2	R	≧98%(EA)	01861-84	10 g
			01861-97	100 g
Albumin, Bovine Serum, Fatty Acid Free, pH7.0	R	≧98%(EA)	08587-26	10 g
			08587-42	25 g
			08578-84	50 g
Amino Acids
L-α-Alanine, Animal-Free	RT	99%(T)	12998-32	25 g
L-Arginine, Animal-Free	RT	98.5%(T)	11984-32	25 g
			11984-45	500 g
L-(+)-Arginine Monohydrochloride, Animal-Free	RT	99%(T)	12999-22	25 g
L-Asparagine Monohydrate, Animal-Free	RT	99%(T)	13000-42	25 g
L-Aspartic Acid, Animal-Free	RT	99%(T)	13002-22	25 g
L-Cysteine Hydrochloride Monohydrate, Animal-Free	R	99%(T)	11983-42	25 g
			11983-55	500 g
L-Cystine Dihydrochloride, Animal-Free	RT	98%(T)	13003-12	25 g
L-Glutamic Acid, Animal-Free	RT	99%(T)	13012-92	25 g
L-Glutamine, Animal-Free	RT	99%(T)	13004-02	25 g
			13004-15	500 g
Glycine, Animal-Free	RT	99%(T)	12997-42	25 g
L-Histidine (free base), Animal-Free	RT	98%(T)	13014-72	25 g
L-Histidine Monohydrochloride Monohydrate, Animal-Free	RT	99%(T)	13017-42	25 g
L-Hydroxyproline [trans-4-Hydroxy-L-proline], Animal-Free	RT	99%(T)	13018-32	25 g
L-(+)-Isoleucine, Animal-Free	RT	99%(T)	13035-02	25 g
L-Leucine, Animal-Free	RT	99%(T)	13036-92	25 g
L-Lysine Monohydrochloride, Animal-Free	RT	99%(T)	13037-82	25 g
L-Methionine, Animal-Free	RT	99%(T)	13038-72	25 g
L-(-)-Phenylalanine, Animal-Free	RT	99%(T)	13039-62	25 g
L-(-)-Proline, Animal-Free	RT	99%(T)	13040-22	25 g
L-Serine, Animal-Free	RT	98%(T)	13041-12	25 g
L-Threonine, Animal-Free	RT	99%(T)	13042-02	25 g
L-Tryptophan, Animal-Free	RT	99%(T)	13043-92	25 g
L-Tyrosine Disodium Salt Dihydrate, Animal-Free	R	98%(T)	11985-22	25 g
			11985-35	500 g
L-Valine, Animal-Free	RT	98%(T)	13046-62	25 g
Vitamins
p-Aminobenzoic Acid, Animal-Free	RT	99%(T)	13047-52	25 g
L(+)-Ascorbic Acid, Animal-Free	RT	99.6%(T)	13048-42	25 g
D-Biotin [Vitamin H], Animal-Free	RT	98%(T)	13049-61	1 g
Choline Chloride, Animal-Free	R	98%(T)	13050-34	5 g
myo-Inositol [Inosite], Animal-Free	RT	99%(GC)	13051-82	25 g
Pyridoxine Hydrochloride, Animal-Free	RT	98%(T)	13053-04	5 g
Vitamin B1 Hydrochloride [Thiamine Hydrochloride], Animal-Free	RT	98%(HPLC)	13052-14	5 g
Vitamin B12, Animal-Free	R	97%(UV)	13054-94	100 mg
Low-MW compounds
17β-Estradiol, Animal-Free	R	≧98.0%(UV)	18192-92	25 mg
IWP-2, Animal-Free	R		18175-64	5 mg
SB 431542, Animal-Free	RT	≧99% (HPLC)	18176-54	5 mg
10mmol/L-Y-27632 Solution	F		18190-96	100 ul
			18190-54	1.5 ml
Y-27632	F	≧98.0%(HPLC)	08954-71	1 mg
			08954-84	5 mg
			08954-42	25 mg
Y-27632, Animal-Free	F	≧98.0%(HPLC)	18188-04	10 mg
Others
D-(+)-Glucose, Animal-Free	RT	98%(GC)	13057-35	500 g
Glutathione(Reduced Form), free acid, Animal-Free	R	97%(T)	13056-61	1 g
Hypoxanthine, Animal-Free	RT	97-102%(UV)	13055-71	1 g
Sodium Pyruvate, Animal-Free	R	97%(T)	13058-12	25 g
Dimethyl Sulfoxide	RT	≧99.5%(GC)	13408-64	5 x 5 ml
Ethanol(99.5)	RT	≧99.5%(GC)	09666-85	500 ml
Sodium Selenite	RT	≧97.0%(T)	11707-04	5 g

(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze

Ordering information

Product Name	Storage	Product No.	PKG Size	Price
2.5g/l-Trypsin Solution	F	35555-54	100 ml
5.0g/l-Trypsin/5.3mmol/l-EDTA Solution	F	35556-44	100 ml
2.5g/l-Trypsin/1mmol/l-EDTA Solution	F	35554-64	100 ml
2.5g/l-Trypsin/1mmol/l-EDTA Solution, with Phenol Red	F	32777-44	100 ml
0.5g/l-Trypsin/0.53mmol/l-EDTA Solution	F	35553-74	100 ml
0.5g/l-Trypsin/0.53mmol/l-EDTA Solution, with Phenol Red	F	32778-34	100 ml
0.2g/l-EDTA Solution	R	14367-74	100 ml

(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze

Ordering information

Product Name	Application	Storage	Product No.	PKG Size	Price
Actinomycin D Solution(1mg/ml)	Other Antibiotics	F	00393-41	1 ml
Antibiotic-Antimycotic Mixed Stock Solution(100x)	Bacteria, Fungal, Yeast	F	02892-54	100 ml
Antibiotic-Antimycotic Mixed Stock Solution(100x)(Stabilized)	Bacteria, Fungal, Yeast	F	09366-44	100 ml
Colcemid Solution(10μg/ml)	Other Antibiotics	R	09356-74	10 ml
G 418 Disulfate	Selection Antibiotics	RT	16512-36	250 mg
			16512-81	1 g
			16512-94	5 g
			16512-52	25 g
G 418 Disulfate	Other Antibiotics	RT	08973-01	1 g
			08973-14	5 g
G 418 Disulfate Aqueous Solution	Selection Antibiotics	R	09380-86	20 ml
			09380-44	100 ml
Gentamicin Sulfate	Bacteria/Mycoplasma	R	08975-81	1 g
			08975-94	5 g
Gentamicin Sulfate Solution (10mg/ml)	Bacteria/Mycoplasma	R	16672-04	10 ml
Gentamicin Sulfate Solution(50mg/ml)	Bacteria/Mycoplasma	R	11980-14	10 ml
Hygromycin B	Selection Antibiotics	R	07296-66	100 mg
			07296-11	1 g
			07296-24	5 g
Hygromycin B Solution	Selection Antibiotics	R	09287-84	20 ml
Kanamycin Monosulfate	Selection Antibiotics	RT	08976-71	1 g
			08976-84	5 g
Kanamycin Sulfate Solution(50mg/ml)	Selection Antibiotics	R	11981-04	20 ml
Mitomycin C Solution(1mg/ml)	Other Antibiotics	F	20898-21	1 ml
Penicillin-Streptomycin Mixed Solution Penicillin 10,000u/ml, Streptomycin 10,000μg/ml	Bacteria(Gram-positive bacteria/ Gram-negative bacteria)	F	26253-84	100 ml
Penicillin-Streptomycin-Glutamine Mixed Solution Penicillin 10,000u/ml, Streptomycin 10,000μg/ml, L-Glutamine 29.2mg/ml, Sodium Chloride 0.14%, Citrate Buffer Solution 10mM	Bacteria(Gram-positive bacteria/ Gram-negative bacteria)	F	06168-34	100 ml
Penicillin-Streptomycin Mixed Solution (Stabilized) Penicillin 10,000u/ml, Streptomycin 10,000μg/ml	Bacteria(Gram-positive bacteria/ Gram-negative bacteria)	F	09367-34	100 ml
Penicillin-Streptomycin Mixed Solution Penicillin 5,000u/ml, Streptomycin 5,000μg/ml	Bacteria(Gram-positive bacteria/ Gram-negative bacteria)	F	26252-94	100 ml
Streptomycin Sulfate	Gram-negative bacteria	R	32204-34	5 g
			32204-92	25 g

(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze