Blocking Reagent (Blocking One Histo)
Features
- Easy to use - eye-drop bottle
- Confirmed - can be used for immunofluorescence staining
- Safe - the preservative does not affect the activity of alkaline phosphatase or horseradish peroxidase
Product Information
Comparison of blocking efficienct with 10% Goat Serum (Immunofluorescence)
In both panels, mouse small intestine tissue section was stained with secondary antibody conjugated with CFTM 488A (green) and counter stained with DAPI (blue). In the panel B with 10% Goat Serum, the stained white arrow along the lines of shape of small intestine show non-specific staining. Blocking One Histo is more effective at reducing non-specific background staining than normal serum.
| Sample | Mouse small intestine (Paraffin-embedded section) |
|---|---|
| Antigen Retrieval | Histo VT One, 90°C, 20 min. |
| Primary antibody | Anti-Vimentin rabbit polyclonal antibody (Santa Cruz: #sc-7557R) |
| Secondary antibody | CFTM 488A Goat anti-Rabbit IgG(H+L), F(ab')2 Fragment(Biotium: #20013) |
Applications
Blocking treatment of each tissue section had been performed by Blocking One Histo. Mouse small intestine (panel A) was stained with anti-PCNA and DAB (3,3'-Diamino Benzidine) to stain nuclear (black arrow), Mouse epididymis (panel B) was stained with anti-Vimentin and DAB to stain muscle (black arrow), Mouse brain (panel C) was stained with anti-GluR and DAB to stain membrane proteins (black arrow) and counter stained with hematoxylin.
A: Mouse small intestine (PCNA) x5
B: Mouse epididymis (Vimentin) x25
C: Mouse brain (GluR) x100
| Antigen Retrieval | Histo VT One, Room temp., 10 min. |
|---|---|
| Primary antibody | A: Anti-PCNA rabbit pAb (Santa Cruz: #sc-7907) B: Anti-Vimentin rabbit pAb (Santa Cruz: #sc-7557R) C: Anti-GluR-1 goat pAb (Santa Cruz: #sc-7608) |
| Secondary antibody | A: Goat anti-rabbit IgG (H+L), biotin conjugated (Vector, #BA-1000) B: Goat anti-rabbit IgG (H+L), biotin conjugated (Vector, #BA-1000) C: Bovine anti-goat IgG (H+L), biotin conjugated (Santa Cruz: #sc-2347) |
| Detection | Streptavidin Biotin Complex Peroxidase Kit (Product No.: 30462) Peroxidase Stain DAB Kit (Brown Stain) (Product No.: 25985) |
Protocols
Blocking One Histo
(PDF 86 KB)
Reference
- K Togami et al.: Journal of Pharmaceutical Sciences 105.3 (2016)
- M Aoki et al.: European Journal of Neuroscience 43.9 (2016)
- R Kawase et al.: Frontiers in Pharmacology 7 (2016)
- Y Kanehira et al.: Journal of Drug Delivery Science and Technology 33 (2016)
- H Doi et al.: Scientific Reports 6 (2016)
- M Takehara et al.: Scientific Reports 6 (2016)
- H Takahashi et al.: Biochemical and Biophysical Research Communications 457.4 (2015)
- M Miyachi et al.: Journal of Clinical Biochemistry and Nutrition 56.3 (2015)
- T Kusudo et al.: FEBS Letters 589.18 (2015)
- Y Umemura et al.: The Journal of Veterinary Medical Science 77.12 (2015)
- I Nanchi et al.: Transgenic Research 24.3 (2015)
- R Mori et al.: The American Journal of Pathology 184.9 (2014)
- Y Mantani et al.: The Journal of Veterinary Medical Science 76.6 (2014)
- T Omotehara et al.: Poultry Science 93.4 (2014)
- J Tokumoto et al: Journal of Veterinary Medical Science75.6 (2013)
- Y Yoneyama et al.: Dental Materials Journal 32.1 (2013)
- M Fukui et al.: Journal of Toxicologic Pathology 26.4 (2013)
- F Yotsumoto et al.: Molecular Cancer Research 11.5 (2013)
- T Takenouchi et al.: Cell Biology International 36.12 (2012)
Downloads
Blocking One Series (PDF 1.85MB)
Ordering Information
| Product Name | Storage | Product No. | PKG Size | Price |
|---|---|---|---|---|
| Blocking One Histo | R | 06349-64 | 50 ml |  |
Related Products
| Product Name | Storage | Product No. | PKG Size | Price |
|---|---|---|---|---|
| Streptavidin Biotin Complex Peroxidase Kit | R | 30462-30 | 1 Kit | |
| Chemi-Lumi One Super | R | 02230-30 | 1 Kit | |
| Chemi-Lumi One L | R | 07880-70 | 1 Kit | |
| WB Stripping Solution | R | 05364-55 | 500 ml | |
| WB Stripping Solution Strong | R | 05677-65 | 500 ml | |
| Peroxidase Stain DAB Kit (Brown Stain) | R | 25985-50 | 1 Kit | |
| Peroxidase Stain DAB Kit (Dark-gray Stain) | R | 25986-40 | 1 Kit | |
| Peroxidase Stain Kit for Immuno-blotting, Nuclease tested | F | 26652-70 | 1 Kit | |
| BCIP-NBT Solution Kit for Alkaline Phosphatase Stain, Nuclease tested | F | 03937-60 | 1 Kit | |
| ELISA POD Substrate A. B. T. S. Kit | R | 14351-80 | 1 Kit | |
| ELISA POD Substrate TMB Kit (Popular) | R (Do not Freze) |
05298-80 | 1 Kit | |
| ELISA POD Substrate TMB Solution (Easy) | R (Do not Freze) |
05299-54 | 100 ml |
(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze
