Serum-free for Slow Freezing Method (Cell Reservoir One)
- Ready-to-use solution
- Serum-free with no animal derived components
- High cell recovery and viability
- Applicable to ES cells (without DMSO)
Product Information
Cell Reservoir One is a serum-free cell culture freezing medium, which contains a water-soluble glycoprotein SERICIN isolated from the silkworm cocoon as a major constituent. SERICIN shows the same high efficacy of cryopreservation as FBS, and reduces the cell toxicity of DMSO. As DMSO is known to have adverse effects on cellular functions, especially Embryonic Stem Cells, Cell Reservoir One is available both with and without DMSO.
Cell viabilities in Cell Reservoir One with DMSO and without DMSO
| Animal Species | Cell Species | Derivation | Freezing Period | Viability rate (%) | |
|---|---|---|---|---|---|
| with DMSO | without DMSO | ||||
| Human | Jurkat | Blood | 1 year | 90 | 85 |
| Human | HL-60 | Blood | 1 year | 90 | 80 |
| Human | WIL2-NS | Spleen | 1 year | 90 | 70 |
| Human | MSC | Bone Marrow | 1 year | 90 | 70 |
| Human | HeLa | Uterine Cervix | 1 year | 90 | 80 |
| Human | HepG2 | Liver | 1 year | 85 | 70 |
| Human | NHDF | Normal Dermis | 1 year | 90 | 75 |
| Mouse | KUSA-A1 | Bone Marrow | 9 months | 90 | 80 |
| Mouse | 2E3-O | Hybridoma | 1 year | 90 | 80 |
| Mouse | P3U1 | Bone Marrow | 1 year | 90 | 80 |
| Mouse | OP9 | Skullcap | 10 months | 95 | 85 |
| Mouse | ES | Embryo | 1 year | 80 | 90 |
| Rat | PC-12 | Adrenal Gland | 1 year | 90 | 70 |
| Hamster | CHO | Ovary | 1 year | 95 | 90 |
| Rabbit | RC4 | Cornea | 1 year | 90 | 70 |
| Insect | Sf-9 | Ovary | 1 year | 75 | 70 |
Application
Application 1
Mouse ES cells: Viability after thawing
Jurkat: Human T Cell Lymphoblast-like Cell Line
mES: Mouse ES Cells
Freezing condition: -80°C, 1 week
The viability of Mouse ES cells in Cell Reservoir One is much higher than the one in FBS with 10%DMSO.
Mouse ES cells: Proliferative ability and undifferentiated state (3 days after thawing)
Freezing condition: -80°C, 1 week Cell: Mouse ES Cells
Protocol
- Cell Reservoir One (with DMSO)
(PDF 86 KB) - Cell Reservoir One (without DMSO)(PDF 86 KB)
References
- OVOL2 Maintains the Transcriptional Program of Human Corneal Epithelium by Suppressing Epithelial-to-Mesenchymal Transition. K Kitazawa et al.: Cell Reports 2016 May 10;15(6):1359-68.
Downloads
Cell Reservoir One (PDF 1 MB)
Ordering information
| Product Name | Storage | Product No. | PKG Size | |
|---|---|---|---|---|
| Cell Reservoir One (with DMSO) | 4°C | 07485-86 | 10 ml |  |
| 07485-44 | 100 ml | |||
| Cell Reservoir One (without DMSO) | 4°C | 07579-37 | 10 ml | |
| 07579-24 | 100 ml | |||
| Cell Reservoir One, Vitrify | R | 11325-62 | 25 ml | |
| Dimethyl Sulfoxide | RT | 13408-64 | 5 x 5 ml | |
| Acetamide | A | 11681-34 | 5 g | |
| Glycerol | RT | 09886-05 | 500 ml | |
| Propylene Glycol | RT | 11740-25 | 500 g |
(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze
