Extra PAGE One Precast Gel
Extra PAGE One Precast Gel is mini-size neutral pH precast gel for SDS-PAGE that offers superior resolution and higher transfer efficiency than conventional precast gels.
Features
- Superior resolution
- Sharp bands
- High transfer efficiency
- Long shelf life: up to 9 months
- Compatible with conventional laemmli sample and running buffers
Product Information
Comparisons
Comparison of Resolution
Extra PAGE One Precast Gel offers higher resolution compared to conventional precast gels. Especially 5-20% gradient gel and 12.5% gel provide improved resolution for low molecular weight.
| Gel | Gradient Gels | Fixed Percentage Gels | ||||
|---|---|---|---|---|---|---|
| Product Name | Extra PAGE One | Company A | Extra PAGE One | Company B | Extra PAGE One | Company A |
| Gel% | 5 - 20% | 4 - 20% | 5 - 20% | 5 - 20% | 12.50% | 12.50% |
| Product No. | 11955-54 | - | 11955-54 | - | 11965-24 | - |
| Migration patterns |  |
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< Sample > Protein Markers (Product No.: 29458-24) 5ul.
< Staining > CBB Stain One (Product No.: 04543）
< Electrophoresis condition > Extra PAGE One Precast Gel: 30mA （constant current） , approx. 50min.
Company A and B: followed the manufacturer’ s instructions
Comparison of Transfer Efficiency-1
Extra PAGE One Precast Gel offers higher transfer efficiency compared to Company A for both high and low molecular weight proteins.
| Sample |
|
| Transfer | Semi-dry with PVDF membrane and Blotting Buffer Solution for Western Blotting (Product No.: 30650-31) (Transfer time 20 min with 10V constant current) |
| Staining | CBB Stain One (Product No.: 04543) |
Comparison of Transfer Efficiency-2
Extra PAGE One Precast Gel offers higher transfer efficiency compared to Company B for high molecular weight proteins.
| Sample |
|
| Transfer | Semi-dry with PVDF membrane and Blotting Buffer Solution for Western Blotting (Product No.: 30650-31) (Transfer time 20 min with 10V constant current) |
| Staining | CBB Stain One (Product No.: 04543) |
Product Lines
Extra PAGE One Precast Gel is available in gradient gels and fixed percentage gels.
| Gel | Gradient Gels | |||
|---|---|---|---|---|
| Gel % | 5 - 10% | 5 - 20% | 7.5 - 15% | 10 - 20% |
| Separation Range | 35 - 450K | 10 - 350K | 15 - 300K | 8 - 200K |
| Product No. 12well/16well | 11953-74 / 11954-64 |
11955-54 / 11956-44 |
11957-34 / 11958-24 |
11959-14 / 11960-74 |
| Migration patterns |  |
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 |
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| Gel | Fixed Percentage Gels | |||
| Gel % | 7.50% | 10% | 12.50% | 15% |
| Separation Range | 40 - 350K | 25 - 250K | 15 - 200K | 10 - 150K |
| Product No. 12well/16well | 11961-64 / 11962-54 |
11963-44 / 11964-34 |
11965-24 / 11966-14 |
11967-04 / 11968-94 |
| Migration patterns |  |
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Note) Back ground may remain slightly when using Nacalai's Rapid Stain CBB Kit (Product No.: 30035-14) or other products. To reduce back ground, wash the gel with 10% acetic acid for 20 minutes.
Specifications
| Cassette Size: | 100mm(W)× 100mm(H) × 3.2mm(T) (Glass) |
| Gel Size: | 80mm(W) × 80mm(H) × 1.0mm(T) |
| Well: | 12wells, 16wells |
| Shelf Life Expire date: | 9 month |
Note) Use electrophoresis tank for plate size 100mm(W) × 100mm(H) × 3.2mm(T).
Applications
Further Analysis
Compatible with MS analysis
Extra PAGE One Precast Gel can be used not only for CBB stain or silver stain but also for MS analysis of bands which cut off from gel.
10% Fixed Percentage Gels
Extra PAGE One Precast Gels 7.5-15% Gels
Bovine Serum Albumin samples (Product No. 01859) were run on SDS-PAGE gels either 10% fixed percentage gel or Extra PAGE One Precast Gel 7.5-15%. The recovered samples from SDS-PAGE gels were digested with trypsin and analyzed by MALDI-MS. The peptide fragments from both gels were clearly detected.
Data courtesy of Associate Professor Tatsuya Moriyama and Dr. Yukihiro Yoshimura, Faculty of Agriculture, Department of Applied Biological Chemistry, Kinki University
Compatible with MS/MS analysis
| Compatible with MS/MS analysis | MS/MS data of band 2 |
|---|---|
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Data Left ： lane A: Protein Ladder One, Triple-color (Product No. ： 09547）
, was applied to Extra PAGE One Precast Gel 5-20%. Band 2,3,5,6,7,10 on
SDS-PAGE were analyzed by MS.
Data right: Protein of band 2 was analyzed by MS/MS, and identified as Glutamate-ammonia-ligase.
| MS/MS data of band 6 | MS/MS data of band 10 |
|---|---|
 |
 |
Protein of band 6 and 10 were also analyzed by MS/MS and identified as 3-dehydroquinate synthase and moaD respectively. All other bands were also identified by MS/MS showing compatibility with MS/MS.
Data courtesy of Mr.Naohiro Matori, Kyoto municipal institute of industrial technology and culture
Downloads
Extra PAGE One Precast Gel 
(PDF 3MB)
Ordering Information
| Product Name | Number of Well | Grade | Storage | Product No. | Price |
|---|---|---|---|---|---|
| Extra PAGE One Precast Gel 5-10% | 13 | SP | R | 13059-44 |  |
| Extra PAGE One Precast Gel 5-10% | 17 | SP | R | 13060-04 | |
| Extra PAGE One Precast Gel 5-15% | 13 | SP | R | 13061-94 | |
| Extra PAGE One Precast Gel 5-15% | 17 | SP | R | 13062-84 | |
| Extra PAGE One Precast Gel 5-20% | 13 | SP | R | 13063-74 | |
| Extra PAGE One Precast Gel 5-20% | 17 | SP | R | 13064-64 | |
| Extra PAGE One Precast Gel 7.5-15% | 13 | SP | R | 13065-54 | |
| Extra PAGE One Precast Gel 7.5-15% | 17 | SP | R | 13066-44 | |
| Extra PAGE One Precast Gel 10-20% | 13 | SP | R | 13067-34 | |
| Extra PAGE One Precast Gel 10-20% | 17 | SP | R | 13068-24 | |
| Extra PAGE One Precast Gel 7.5% | 13 | SP | R | 13069-14 | |
| Extra PAGE One Precast Gel 7.5% | 17 | SP | R | 13070-74 | |
| Extra PAGE One Precast Gel 10% | 13 | SP | R | 13071-64 | |
| Extra PAGE One Precast Gel 10% | 17 | SP | R | 13072-54 | |
| Extra PAGE One Precast Gel 12.5% | 13 | SP | R | 13073-44 | |
| Extra PAGE One Precast Gel 12.5% | 17 | SP | R | 13074-34 | |
| Extra PAGE One Precast Gel 15% | 13 | SP | R | 13075-24 | |
| Extra PAGE One Precast Gel 15% | 17 | SP | R | 13076-14 |
(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze
