Serum Substitutes for Neural Stem Cell Culture N-N-2 Supplement (100X)

N-2 Supplement (100X) is a serum substitute used for culturing neural cells. It enables the maintenance of undifferentiated neural stem cells, which would otherwise undergo differentiation when exposed to serum. In addition, it can be used as a supplement for a variety of neural cell cultures in combination with growth factors and other serum substitutes.

Features

Enhances neural stem cell growth and expansion
Xeno-free

Product Description 1

Ingredients

Recombinant human insulin
Human holo-transferrin (iron-saturated)
Sodium selenite
Putrescine
Progesterone

Culturing neural cells using the neurosphere method

A single-cell suspension was prepared from the brains of E14 mouse embryos and cultured using the neurosphere method in medium* supplemented with either this product or a competitor’s product (Company A). Evaluation of cell morphology and proliferation rate showed that this product achieved results comparable to those of Company A’s product.

* Medium: DMEM/Ham's F-12 (#08460-95), 20 ng/mL bFGF, 20 ng/mL EGF

Cell morphology (Day 6 of culture)

Cell proliferation assay (WST-8 method)

Product Description 2

Culturing neural cells using the adherent culture method

A single-cell suspension was prepared from the brains of E10 mouse embryos and cultured on, Poly-L-lysine‑coated plates using medium* supplemented with either this product or a competitor’s product (Company A). Results demonstrated that this product, like Company A's product, supports the proliferation of cells while maintaining them in an undifferentiated state.

* Medium: DMEM/Ham’s F-12(#08460-95), 20 ng/mL bFGF, 20 ng/mL EGF

Comparison of cell morphology and growth

After 4 days of culture, evaluation of cell morphology and cell number showed that this product produced results comparable to those of Company A’s product.

Confirmation of the undifferentiated state

Immunostaining was performed on cells after 3 days of culture to examine the expression of Ki67 (a proliferation marker) and Pax6 (a neural stem/progenitor cell marker). No significant differences were observed in the number of marker-positive cells between cultures supplemented with this product and those with Company A's product, indicating that the cells maintained an undifferentiated state.