Supercritical Fluid Chromatography (SFC), which uses a supercritical fluid as the mobile phase, has gained attention in recent years. Compared to HPLC, SFC uses less organic solvent, produces fractions that are easier to purify due to quick solvent evaporation, and exhibits different retention behavior. SFC columns respond differently to changing analysis conditions compared to LC columns. We introduce some common adjustments in this technical note.

 

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