COSMOSIL SL-II

Features

Column Image

High purity silica gel (>99.99%) with special treatment
Suitable for preparative separation

Product Information

Specifications

Packing Material	SL-II
USP Code	L3
Silica Gel	High Purity Porous Spherical Silica
Average Particle Size	3, 5, 15 µm
Average Pore Size	approx. 120 Å
Specific Surface Area	approx. 300 m²/g
Stationary Phase	High purity Silica Gel (>99.99%) with specially treatment Suitable for preparative separation

Comparison with Old Type

COSMOSIL SL-II with high purity silica gel offers better peak shape for phenols with simple mobile phase of ethanol or hexane. No acetic acid additives were required, unlike for the old type silica.

Analysis of acids and amide without ionic additives

Condition
Column Size	4.6 mm I.D. × 250 mm	Detection	UV 254 nm, 0.5AUFS
Mobile Phase	Methanol : Hexane = 10 : 90	Sample	Benzoic Acid4.0 µg Salicylic Acid6.0 µg Salicylamide4.0 µg
Flow Rate	1 ml/min
Temperature	30°C

Comparison with Medium-pressure Columns

COSMOSIL SL-II offers sharper peak compared with packing materials for medium-pressure liquid chromatography and open chromatography.

Column	5SL-II	A Company Cartridge (30 µm silica gel)	B Company Cartridge (60 µm silica gel)
Column Size	20 mm I.D. x 250 mm	26 mm I.D. x 104 mm	26 mm I.D. x 104 mm
Pressure	1.3 MPa	0.25 MPa	0.15 MPa
Chromatogram

Condition
Mobile Phase	Ethanol : Hexane = 5 : 95	Sample	p-Xylene8.3 mg Cinnamyl Alcohol 1.7 mg p-Nitrobenzyl Alcohol4.2 mg
Flow Rate	10 ml/min
Temperature	Room Temperature
Detection	UV 254 nm

Scaling Up from Analytical to Preparative Separation

Normal phase chromatography, with non-polar mobile phase and low boiling point is used for preparative separation because solvent removal is generally easier for normal phase chromatography than for reversed chromatography. The followings are how to scale up from analytical (4.6 mm I.D.) to preparative (20.0 mm I.D.) separation.

Step 1	Step 2	Step 3
Mobile phase is optimized by using 4.6 mm I.D. × 250 mm column	Maximum load capacity is optimized by using 4.6 mm I.D. × 250 mm column	Preparative separation by using 20 mm I.D. × 250 mm column
As mobile phase, highly soluble solvent for sample is suitable.	Maximum injection volume 56 µl (28 mg)	Maximum injection volume 1,050 µl (525 mg) (19 times higher than 4.6 mm I.D.)

Condition
Mobile Phase	Ethyl Acetate : Hexane = 10 : 90	Sample	1.1-Naphthol
Flow Rate	4.6 mm I.D. : 1 ml/min 20 mm I.D. : 18.9 ml/min		1.1-Naphthol
Temperature	Room Temperature		2.2-Naphthol
Detection	UV 254 nm		2.2-Naphthol

Inner Diameter of Column(scale down and scale up) (PDF 344 KB)

Applications

COSMOSIL Application Search

Downloads

Brochures

COSMOSIL SL-II (0.7 MB)

References

Reference list

COSMOSIL SL-II reference list

Ordering Information

Analytical and Preparative Column (Particle size : 5 µm)

COSMOSIL 5SL-II

Product Name	Size	Product No.
COSMOSIL 5SL-II Guard Column
	4.6 mm l.D.×10 mm	37997-01
	10 mm l.D.×20 mm	37998-91
	20 mm l.D.×20 mm	05874-91
	20 mm l.D.×50 mm	05875-81
	28 mm l.D.×50 mm	34359-51
COSMOSIL 5SL-II Packed Column
	4.6 mm l.D.×50 mm	37999-81
	4.6 mm l.D.×100 mm	38000-01
	4.6 mm l.D.×150 mm	38001-91
	4.6 mm l.D.×250 mm	38002-81
	6.0 mm l.D.×150 mm	38003-71
	6.0 mm l.D.×250 mm	38004-61
	10 mm l.D.×250 mm	38005-51
	20 mm l.D.×250 mm	38006-41
	28 mm l.D.×250 mm	34358-61

Preparative Column (Particle size : 15 µm)

COSMOSIL 15SL-II

Product Name	Size	Product No.
COSMOSIL 15SL-II Guard Column
	28 mm l.D.×50 mm	05892-51
	50 mm l.D.×50 mm	05894-31
COSMOSIL 15SL-II Packed Column
	28 mm l.D.×250 mm	05893-41
	50 mm l.D.×250 mm	05895-21
	50 mm l.D.×500 mm	05896-11