COSMOSIL PYE

Features

Column Image

Pyrenylethyl group bonded stationary phase
Separation with high molecular shape selectivity or π-π interactions
Excellent separation for structural isomers

Product Information

Column Specifications

Comparison of π-π Interactions with πNAP

COSMOSIL PYE provides much stronger π-π interactions than πNAP.

pi-pi_interaction

stationary_phase

Figure. Comparison of π-π interactions

Applications

Sterol

Condition
Column Size	4.6 mm I.D.x150 mm
Flow Rate	1.0 ml/min
Temperature	30°C
Detection	UV 210 nm
Sample	Cholesterol Stigmasteroll Campesterol Sitosterol

Phthalonitrile

Condition
Column Size	4.6 mm I.D. x 150 mm
Flow Rate	1.0ml/min
Temperature	30°C
Detection	UV 254 nm
Sample	o-Phthalonitrile (Phthalonitrile) 0.3 µg m-Phthalonitrile (Isophthalonitrile) 3.0 µg p-Phthalonitrile (Terephthalonitrile) 0.15 µg

Tocopherol

Condition
Column Size	4.6 mm I.D. x 150 mm
Flow Rate	1.0ml/min
Temperature	30°C
Detection	UV 295 nm
Sample	α-Tocopherol 1.0 µg β-Tocopherol 1.0 µg γ-Tocopherol 1.0 µg δ-Tocopherol 1.0 µg

Capsaicins

Condition
Column Size	4.6 mm I.D. x 150 mm
Mobile phase	Methanol/ H2O = 70/30
Flow Rate	1.0ml/min
Temperature	30°C
Detection	UV 280 nm
Sample	Capsaicin (1.0µg) N-Vanillylnonanamide (0.5µg)

Attention

Methanol is recommended as a mobile phase for COSMOSIL PYE column. Acetonitrile is not recommended because it has many π electrons and interferes π-π interactions between a sample and the stationary phase.
The stationary phase of COSMOSIL PYE, pyrenylethyl group, has a large UV absorption. When the stationary phase detaches from silica gel and elutes, even a slight quantity can be detected and causes baseline noise. In such a case, wash the column with tetrahydrofuran. Detachment of a small amount of the stationary phase does not deteriorate a column’s separation ability.
COSMOSIL PYE column is not suitable for gradient analysis.

See more COSMOSIL Applications

Usage information

Column	5 µm PYE
I.D. (mm)	2.0	3.0	4.6	10.0	20.0
Washing method	1. Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. For example, if your mobile phase was 50:50 methanol/20 mmol/l phosphate buffer, wash with 50:50 methanol/water. 2. (If you used a buffer, always do step 1 first!) Remove adsorbed compounds and fix unstable baselines by washing with methanol and/or THF.
Storage conditions	Short-term (up to a week): Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. For example, if your mobile phase was 50:50 methanol/20 mmol/l phosphate buffer, wash with 50:50 methanol/water. Long-term: Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. Then, replace the solvent with 70% methanol or acetonitrile : 30% water. In either case, tightly plug the column, and store in a cool and dry place.
Usable pH range	pH2～pH7.5
Max. pressure	20MPa			15MPa
Temperature range	The maximum usable temperature is 60°C. However. for regular use, please use at a constant temperature between 20°C and 50°C.
Usable solvents	Any solvent that will not dissolve the silica gel (such as alkaline solutions) or cleave the stationary phase (such as very acidic solutions) is usable. Note: For solvents with high viscosity, please watch the system pressure and keep it below 20 MPa. Also, please wash the column after using acidic mobile phases or solvents with high freezing points.
Other instructions	- Buffer concentration is usually sufficient at 0.005 - 0.02 mol/L. - Always filter mobile phases using a 0.5 µm or finer filter before use. - As acetonitrile interferes with π electron interactions, please use a methanol-based mobile phase. - The baseline may become unstable due to the high UV absorption of shedded stationary phase. If this occurs, please wash the column with THF. A small amount of stationary phase shedding will not affect performance. - This column is not suitable for gradient analysis.

Downloads

Brochures

COSMOSIL PYE/NPE (2.7 MB)

Posters

Evaluation of Pentabromobenzyl Stationary Phase for Separation of Polar Compounds by Reversed-Phase Chromatography
(Poster from HPLC 2015, Geneva)
(370 KB)

References

Reference list

Ordering Information

Analytical Column (Particle Size : 5 µm)
COSMOSIL PYE

Product Name	Size	Product No.
COSMOSIL 5PYE Guard Column
	4.6 mm I.D. x 10 mm	37903-11
	10 mm I.D. x 20 mm	38041-71
	20 mm I.D. x 20 mm	05867-91
	20 mm I.D. x 50 mm	34475-21
COSMOSIL 5PYE Packed Column
	1.0 mm I.D. x 150 mm	02851-71
	2.0 mm I.D. x 150 mm	38042-61
	2.0 mm I.D. x 250 mm	34450-31
	4.6 mm I.D. x 150 mm	37837-91
	4.6 mm I.D. x 250 mm	37989-11
	10 mm I.D. x 250 mm	37996-11
	20 mm I.D. x 250 mm	38044-41