Products

COSMOSIL Diol Series for SEC

Features

Column Image

  • Separate large hydrophilic molecules, such as proteins, polysaccharides, and nucleic acids, by molecular size
  • Reduce undesirable adsorption
  • High pressure tolerance with silica gel-based packing material
  • Different products cover a wide range of molecular weights

Product Information

Specifications

Packing Material 5Diol-120-II 5Diol-300-II 5Diol-1000-II 5Diol-2000-II
USP Code L20
Silica Gel High Purity Porous Spherical Silica (1)
Average Particle Size 5 µm
Average Pore Size approx. 120 Å approx. 300 Å approx. 1000 Å approx. 2000 Å
Stationary Phase Diol Group
Suitable Samples Protein, Water-Soluble Polymer
Flow Rate 0.5–1.0 (ml/min)
Sample MW (Da) Protein 5,000–100,000 10,000–700,000 — —
Water-Soluble Polymer 1,000–20,000 5,000–100,000 50,000–500,000 —

(1) With the silica-based gel, organic solvents, including methanol and acetonitrile, can be used.

Calibration Curve of Proteins

Condition
Column COSMOSIL 5Diol-II 7.5 mm I.D. x 600 mm
Mobile Phase 20 mmol/l Phosphate Buffer(pH7.0)+100 mmol/l Na2SO4
Flow Rate 1.0 ml/min
Temperature 30°C
Detection UV 220 nm

Sample

Sample Name M.W. Sample Name M.W.
Thyroglobulin 660,000 Albumin (Ovalbumin) 45,000
Catalase 250,000 Peroxidase 40,000
Glucose Oxidase 160,000 Carbonic Anhydrase 30,000
Uricase 128,000 α-Chymotrypsinogen A 25,700
Choline Oxidase 95,000 α-Chymotrypsin 25,200
Transferrin 85,000 Trypsinogen 24,000
Conalbumin 77,500 Trypsin (bovine) 23,300
Malate Dehydrogenase 70,000 Myoglobin 17,000
α-Glucosidase 68,500 Lysozyme 14,300
Albumin (BSA) 66,000 Ribonuclease A 13,700
α-Amylase 52,500 Cytochrome C 12,400
Fetuin 48,000 Aprotinin 6,500
Gly-Gly 132

Linear pullulan calibration curve

Condition
Column COSMOSIL 5Diol-II 7.5 mm I.D. x 300 mm
Mobile Phase Water
Flow Rate 1.0 ml/min
Temperature 30°C
Detection RI

Sample

Linear pullulan M.W.
P-800 853,000
P-400 380,000
P-200 186,000
P-100 100,000
P-50 48,000
P-20 23,700
P-10 12,200
P-5 5,800
Maltotriose 504
Maltose 342
Glucose 180

Molecular Weight Range

Globular molecules have smaller apparent size compared to linear molecules of the same weight. Therefore, globular molecules can be separated with smaller pores compared to linear molecules.

Comparison with Other Companies Columns

COSMOSIL Diol-II demonstrates high performance compared to other companies' columns.

Condition
Column COSMOSIL 5Diol-300-II 7.5 mm I.D. x 300 mm Sample
  1. Transferrin
  2. Hemoglobin, Human
  3. Angiotensin II, Human
Mobile Phase 20 mmol/l Phosphate Buffer (pH 7.0) + 100 mmol/l Na2SO4
Flow Rate 1.0 ml/min
Temperature 30°C
Detection UV 220 nm

Applications

DNA

Conditions
Column Size 7.5 mm I.D. x 300 mm Sample
  1. dsDNA, 1000bp
  2. dsDNA, 100bp
  3. ssDNA, 20mer
  4. Uracil
Mobile Phase 20 mmol/l Phosphate Buffer (pH 7.0) + 100 mmol/l Na2SO4
Flow Rate 1.0 ml/min
Temperature 30°C
Detection UV 260 nm

Polyvinyl Alcohol

Conditions
Column 5Diol-300-II
Size 7.5 mm I.D. - 300 mm
Mobile Phase H2O
Flow Rate 1.0 ml/min
Temperature 30°C
Detection RI
Sample Polyvinyl Alcohol (10mg/ml)
Inj. Vol. 10 µl

Methyl Cellulose

Conditions
Column 5Diol-1000-II
Size 7.5 mm I.D. - 300 mm
Mobile Phase H2O
Flow Rate 1.0 ml/min
Temperature 30°C
Detection RI
Sample Methyl Cellulose (5mg/ml)
Inj. Vol. 20 µl

Dextrin

Conditions
Column 5Diol-300-II Temperature 30°C
Column Size 7.5 mm I.D. x 300 mm Detection RI
Mobile Phase H2O Sample Dextrin (10 mg/ml)
Flow Rate 1.0 ml/min Inj. Vol. 10 µl

Inulin

Conditions
Size 7.5 mm I.D. - 300 mm
Mobile Phase H2O
Flow Rate 1.0 ml/min
Temperature 30°C
Detection RI
Sample Inulin from Dahlia Tubers (10 mg/ml)
Inj. Vol. 10 µl

Laminarin

Conditions
Size 7.5 mm I.D. - 300 mm
Mobile Phase H2O
Flow Rate 1.0 ml/min
Temperature 30°C
Detection RI
Sample Laminaran from Eisenia Bicyclis
(5.0 mg/ml)
Inj. Vol. 20 µl

Gum Arabic

Conditions
Size 7.5 mm I.D. - 300 mm
Mobile Phase 100mmol/l Na2SO4
Flow Rate 1.0 ml/min
Temperature 30°C
Detection RI
Sample Acacia [Gum Arabic] (10 mg/ml)
Inj. Vol. 10 µl

Polystyrene

chromatogram of polystyrene

Conditions
Size 4.6 mm I.D. - 250 mm
Mobile Phase Tetrahydrofuran
Flow Rate 0.4 ml/min
Temperature 30°C
Detection UV 254 nm
Sample
  1. Polystyrene, MW 1,800,000
  2. Polystyrene, MW 170,000
  3. Polystyrene, MW 17,000
  4. Benzene
Inj. Vol. 4.0 µl

COSMOSIL Application Search

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References

Reference list

Ordering Information

COSMOSIL 5Diol-120-II

Product Name Size Product No. Price
COSMOSIL 5Diol-120-II Guard Column
7.5 mm l.D. x 50 mm 38049-91 e-Nacalai
COSMOSIL 5Diol-120-II Guard Cartridge
Guard cartridge holder is required.		 4.6 mm l.D. x 10 mm 19179-94 e-Nacalai
COSMOSIL 5Diol-120-II Packed Column
7.5 mm l.D. x 300 mm 38050-51 e-Nacalai
7.5 mm l.D. x 600 mm 38051-41

COSMOSIL 5Diol-300-II

Product Name Size Product No. Price
COSMOSIL 5Diol-300-II Guard Column
7.5 mm l.D. x 50 mm 38052-31 e-Nacalai
COSMOSIL 5Diol-300-II Guard Cartridge
Guard cartridge holder is required.		 4.6 mm l.D. x 10 mm 19180-54 e-Nacalai
COSMOSIL 5Diol-300-II Packed Column
7.5 mm l.D. x 300 mm 38053-21 e-Nacalai
7.5 mm l.D. x 600 mm 38054-11

COSMOSIL 5Diol-1000-II

Product Name Size Product No. Price
COSMOSIL 5Diol-1000-II Guard Column
7.5 mm l.D. x 50 mm 13337-81 e-Nacalai
COSMOSIL 5Diol-1000-II Packed Column
7.5 mm l.D. x 300 mm 13338-71 e-Nacalai

COSMOSIL 5Diol-2000-II

Product Name Size Product No. Price
COSMOSIL 5Diol-2000-II Packed Column 7.5 mm I.D. x 300 mm 19976-31 e-Nacalai

(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze