Products

COSMOSIL PFP

Features

Column Image

  • Pentafluorophenyl-bonded stationary phase
  • Alternative selectivity to C18 columns
  • Available in analytical and preparative columns
  • Suitable for structural isomers and halogenated compounds

Product Information

Column Specifications

Column Specifications

Alternative Selectivity to C18 Columns

COSMOSIL PFP provides different selectivity from C18 Columns. Furthermore it offers improved separation compared to other companies’ PFP columns.

Application

Condition
Column size 4.6 mm I.D. x 150 mm Sample o-Cresol (3.0mg/ml)
m-Cresol (3.0mg/ml)
p-Cresol (3.0mg/ml)
Mobile phase Methanol/ H2O = 40/60
Flow Rate 1 ml/min Inj.Vol. 1.0 µl
Temperature 30°C Structure
Detection UV 254 nm

Reasonably Priced Preparative Columns

We can offer preparative columns at a reasonable price because we synthesize our own silylating agents.

Column size 4.6mmI.D.×250mm 10mmI.D.×250mm 20mmI.D.×250mm
HPLC System Analytical HPLC System Analytical HPLC System Semi-Preparative HPLC System
Flow rate 1.0ml/min 5.0ml/min 19.0ml/min
Injection vol. 10 µl 50 µl 190 µl
Chromatogram
Condition
Mobile phase Methanol/ H2O = 70/30 Sample
  1. Uracil
  2. Methyl Benzoate
  3. Toluene
  4. Naphthalene
Temperature 30°C
Detection UV 254 nm

Separation Properties of PFP

Structure

COSMOSIL PFP uses dipole-dipole and π-π interactions. It offers strong retention for compounds with strong dipole moments, e.g. cationic or halogenated compounds. It also has high steric selectivity for structural isomer separations.

Applications

Condition
Column size 4.6mmI.D.-150mm Sample o-Dinitrobenzene
m-Dinitrobenzene
p-Dinitrobenzene
Benzene (I.S.)
Mobile phase Methanol/ H2O = 50/50
Flow rate 1.0 ml/min
Temperature 30°C Structure
Detection UV254nm

Applications

Fluorinated compounds

Condition
Column size 4.6mmI.D.-150mm Sample
  1. Benzene (2.5mg/ml)
  2. Fluorobenzene (1.0mg/ml)
  3. Toluene (2.5mg/ml)
  4. α,α,α-Trifluorotoluene [Benzotrifluoride] (0.25mg/ml)
Mobile phase Methanol/ H2O = 60/40
Flow rate 1.0 ml/min Inj. Vol. 1.0 µl
Temperature 30°C Structure
Detection UV 254 nm

Vitamin E

Condition
Column Size 4.6 mm I.D. x 250 mm
Mobile Phase Methanol/ H2O = 90/10
Flow Rate 1.0 ml/min
Temperature 30°C
Detection UV 254 nm
Sample
  1. δ-Tocotrienol
  2. β-Tocotrienol
  3. γ-Tocotrienol
  4. α-Tocotrienol
  5. δ-Tocopherol
  6. β-Tocopherol
  7. γ-Tocopherol
  8. α-Tocopherol

Cresol Isomers

Condition
Column Size 4.6 mm I.D. x 250 mm
Mobile Phase Methanol/ H2O = 40/60
Flow Rate 1.0 ml/min
Temperature 30°C
Detection UV 254 nm
Sample o-Cresol (3.0mg/ml)
m-Cresol (3.0mg/ml)
p-Cresol (3.0mg/ml)
Inj.Vol. 1.0 µl
Structure

See more COSMOSIL Applications

Usage information

Column 5 µm PFP
I.D. (mm) 2.0 3.0 4.6 10.0 20.0 28.0
Washing method 1. Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. For example, if your mobile phase was 50:50 methanol/20 mmol/l phosphate buffer, wash with 50:50 methanol/water.
2. (If you used a buffer, always do step 1 first!) Remove adsorbed compounds and fix unstable baselines by washing with methanol and/or THF.
Storage conditions Short-term (up to a week):
Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. For example, if your mobile phase was 50:50 methanol/20 mmol/l phosphate buffer, wash with 50:50 methanol/water.

Long-term:
Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. Then, replace the solvent with 70% methanol or acetonitrile : 30% water.

In either case, tightly plug the column, and store in a cool and dry place.
Usable pH range pH2～pH7.5
Max. pressure 20MPa 15MPa
Temperature range The maximum usable temperature is 60℃. However. for regular use, please use at a constant temperature between 20°C and 50°C.
Usable solvents Any solvent that will not dissolve the silica gel (such as alkaline solutions) or cleave the stationary phase (such as very acidic solutions) is usable.
Note: For solvents with high viscosity, please watch the system pressure and keep it below 20 MPa. Also, please wash the column after using acidic mobile phases or solvents with high freezing points.
Other instructions - Buffer concentration is usually sufficient at 0.005 - 0.02 mol/L.
- Always filter mobile phases using a 0.5 µm or finer filter before use.
- Acetonitrile is not recommended as a mobile phase.

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Ordering Information

Analytical and Preparative Column (Particle size : 5 µm)

COSMOSIL 5PFP

Product Name Size Product No. Price
COSMOSIL 5PFP
Guard Column
4.6 mm I.D. x 10 mm
cartridge*		 12443-24 Inquire
Now
10.0 mm I.D. x 20.0 mm 12385-81
20.0 mm I.D. x 20.0 mm 13275-91
COSMOSIL Guard
Cartridge Holder		 4.6 mm I.D. 38009-79
COSMOSIL 5PFP
Column
2.0 mm l.D. x 50 mm 13263-41 Inquire
Now
2.0 mm l.D. x 100 mm 13264-31
2.0 mm l.D. x 150 mm 12381-21
2.0 mm l.D. x 250 mm 13265-21
3.0 mm l.D. x 50 mm 13266-11
3.0 mm l.D. x 100 mm 13267-01
3.0 mm l.D. x 150 mm 13268-91
3.0 mm l.D. x 250 mm 13269-81
4.6 mm l.D. x 50 mm 13270-41
4.6 mm l.D. x 100 mm 13271-31
4.6 mm l.D. x 150 mm 12383-01
4.6 mm l.D. x 250 mm 12384-91
10.0 mm l.D. x 50 mm 13272-21
10.0 mm l.D. x 100 mm 13273-11
10.0 mm l.D. x 150 mm 13274-01
10.0 mm l.D. x 250 mm 12386-71
20.0 mm l.D. x 50 mm 13276-81
20.0 mm l.D. x 100 mm 13277-71
20.0 mm l.D. x 150 mm 13278-61
20.0 mm l.D. x 250 mm 12387-61
28.0 mm l.D. x 100 mm 13280-11
28.0 mm l.D. x 150 mm 13281-01
28.0 mm l.D. x 250 mm 13282-91

*Cartridge Holder is required.

(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze