COSMO(+)Capillary
COSMO(+)Capillary is cationic polymer coated capillary column, for electroosmotic flow (EOF) is from the chthode (-) to the direction of the anode (+) because of the cationic polymer coating inside of the capillary column.
- Opposit EOF direction to non-coated capillary
- High reproducibility
- Applicable wide pH range (pH2-10)
Comparison of COSMO(+)Capillary and Non-coated Capillary
Electropherograms for E.coli Metabolic Extraction
The figure shows the analysis data of various kinds of metabolites. COSMO(+)Capillary can analyze them within 20 minutes by electroosmotic flow (EOF) reversal and a sheath liquid consisting of 50 mM ammonium acetate, pH8.5.
Peak Identification:
1: Glycolate
2: Lactate
3: Fumarate
4: 2-Oxoisopentanoate
5: Succinate
6: Malate
7: PEP
8: DHAP
9: Glycerol3P
10: 3PG
11: MES(is)
12: Gluconate
13: Ribulose5P
14: G1P
15: G6P+F6P
16: 2,3DPG
17: Sedoheptulose7P
18, F1,6DP
Data courtesy of Prof. Tomoyoshi Soga, Institute for Advanced Biosciences (IAB), Keio University
| Experimental Conditions: | |||
|---|---|---|---|
| Sample: Capillary: Electrolyte: Applied Potential: Injection: Temperature: Sheath liquid: |
E.coli W3110, 109 Cells COSMO(+) 50 µm I.D. x 110 cm 50 mM Ammonium Acetate, pH8.5 -30 kV 30 sec at 50 mbar 20°C 10 µl/min 5 mM Ammonium Acetate in 50% (v/v) Methanol - Water |
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Application Data
The figure shows high reproducibility when COSMO(+)Capillary is used repetitively (26 reference standards).
Data courtesy of Prof. Tomoyoshi Soga, Institute for Advanced Biosciences (IAB), Keio University
| Experimental Conditions: | |||
|---|---|---|---|
| Sample Concentration Capillary Electrolyte Applied Potential Injection Temperature Sheath liquid |
50 µmol/l each COSMO(+) 50 µm I.D. x 110 cm 50 mM Ammonium Acetate, pH8.5 -30 kV 30 sec at 50 mbar 20°C 10 µl/min 5 mM Ammonium Acetatein 50% (v/v) Methanol - Water |
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The COSMO(+)Capillary enables separation of Succinic Acid which fused silica can not separate. Furthermore it has shorter analysis time for other acids than fused silica.
(a) Lactic Acid (m/z=89)
(b) Succinic Acid (m/z=117)
(c) Malic Acid (m/z=133)
(d) Uric Acid (m/z=167)
(e) Citrate (m/z=191)
Data courtesy of Yoshihide Tanaka, National Institute of Advanced Industrial Science and Technology
| Experimental Conditions: | ||||
|---|---|---|---|---|
| Capillary Electrolyte Applied Potential Injection Temperature Sheath liquid |
COSMO(+) 50 µm I.D. x 110 cm 50 mM ammonium acetate, pH8.5 -30 kV -10 kV × 30 sec 25°C 10 mM Ammonium Carbonate: 2-Propanol =1:1 |
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Ordering information
| Product Name | PKG size | Product No. |
|---|---|---|
| COSMO(+)Capillary (50 µm I.D. x 120 cm) | 2 PKG | 07584-44 |
