COSMOSIL Index

COSMOSIL Ultra-High Performance Columns

Ultra-High Performance Chromatography is a powerful tool for very fast and efficient separation.
Column Images

Specifications

Packing Material 2.5
C18-MS-II		 2.5
Cholester		 2.5
πNAP		 2.5
HILICnew
USP Code  L1 L101    L104 
Silica Gel High Purity Porous Spherical Silica
Average Particle Size 2.5 µm
Average Pore Size approx. 130 Å
Specific Surface Area approx. 330 m2/g
Stationary Phase HILIC
Octadecyl Group Cholesteryl Group Naphtylethyl
Group		 Triazole
Bonding Type Monomeric -
Main Interaction Hydrophobic Interaction Hydrophobic Interaction
Molecular Shape Selectivity		 Hydrophobic Interaction
π-π Interaction		 Hydrophilic Interaction, Anion Exchange
End Capping Treatment Near-perfect Treatment -
Features - Multi-purpose C18 column
- Suitable for  basic compounds.
 - Usable under the same condition as C18.
-Strong molecular shape selectivity 		- Stronger π-π interaction than Phenyl columns. -Suitable for non-retaining by C18

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COSMOSIL 2.5C18-MS-II

  • Monomeric C18 phase for multi-purpose separations
  • Low back pressure
Pressure Comparison with Competitor's 2 µm Columns
3.0 mm I.D . x 75 mm
2.5C18-MS-II Competitor's 2 µm C18
2.5Cholester Competitor's 2 μm C18
Condition
Column Size 3.0 mm I.D . x 75 mm Sample
  1. Benzene (1.67 mg/ml)
  2. Toluene (1.67 mg/ml)
  3. Ethylbenzene (1.67 mg/ml)
  4. Propylbenzene (1.67 mg/ml)
  5. Butylbenzene (1.67 mg/ml)
  6. Amylbenzene (1.67 mg/ml)
Mobile Phase Acetonitrile : H2O = 70 : 30
Flow Rate 1 ml/min
Temperature 40 °C
Detection UV 254 nm Injection Vol. 1.0 µl
2.0 mm I.D . x 50 mm
2.5C18-MS-II Competitor's 2 μm C18
2.5Cholester Competitor's 2 μm C18
Condition
Column Size 2.0 mm I.D . x 50 mm Sample
  1. Benzene (1.67 mg/ml)
  2. Toluene (1.67 mg/ml)
  3. Ethylbenzene (1.67 mg/ml)
  4. Propylbenzene (1.67 mg/ml)
  5. Butylbenzene (1.67 mg/ml)
  6. Amylbenzene (1.67 mg/ml)
Mobile Phase Acetonitrile : H2O = 70 : 30
Flow Rate 0.4 ml/min
Temperature 40 °C
Detection UV 254 nm Injection Vol. 0.5 µl

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See more COSMOSIL Applications

Brochures

  • COSMOSIL Ultra-High Performance Columnpdf (1.2 MB)

Ordering Information

COSMOSIL 2.5C18-MS-II
Product Name Size Product No.  
COSMOSIL 2.5C18-MS-II Packed Column
2.0 mm l.D. x 50 mm 08994-31 e-Nacalai
2.0 mm l.D. x 75 mm 08995-21
2.0 mm l.D. x 100 mm 08996-11
3.0 mm l.D. x 50 mm 08997-01
3.0 mm l.D. x 75 mm 08998-91
3.0 mm l.D. x 100 mm 08999-81

COSMOSIL C18-MS-II (Particle size : 3 ・ 5 ・15 µm) is also available.

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COSMOSIL 2.5Cholester

  • Unique rigid Cholesteryl structure
  • Usable under the same condition as ODS
  • 2.5 µm silica packing material

Improved Separation

COSMOSIL 2.5Cholester offers improved resolution for compounds difficult to analyze with C18 without changing analytical condition.

Separation of Catechins
2.5Cholester Competitor's 2 µm C18
Catechin by 2.5Cholester Catechin by 2μm C18
Condition
Column Size 3.0 mm I.D. x 75 mm Sample
  1. Gallocatechin(GC) (0.40 mg/ml)
  2. Caffeine (0.04 mg/ml)
  3. Epigallocatechin(EGC) (0.40 mg/ml)
  4. Catechin (C) (0.20 mg/ml)
  5. Epicatechin(EC) (0.20 mg/ml)
  6. Epigallocatechin Gallate(EGCG) (0.10 mg/ml)
  7. Gallocatechin Gallate(GCG) (0.20 mg/ml)
  8. Epicatechin Gallate(ECG) (0.10 mg/ml)
  9. Catechin Gallate(CG) (0.10 mg/ml)
Mobile Phase A: Acetonitrile : 20 mmol/l Phosphate Buffer (pH2.5)= 10 : 90
B: Acetonitrile : 20 mmol/l Phosphate Buffer (pH2.5)= 30 : 70
B: 0→ 100%/ 5 min Linear Gradient
Mixer 0.5 ml
Flow Rate 1 ml/min
Temperature 40 °C
Detection UV 280 nm Injection Vol. 1.0 µl

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Separation of Saikosaponins
2.5Cholester （2.0 mm I.D. x 50 mm） Competitor's 1.7 µm C18（2.1 mm I.D. x 50 mm）
2.5Cholester （2.0 mm I.D. x 50 mm） Competitor's 1.7 μm C18 （2.1 mm I.D. x 50 mm)
Condition
Mobile Phase Acetonitrile: 0.05%NaH2PO4 aq. = 30 : 50 Sample
  1. Saikosaponin c
  2. Saikosaponin h
  3. Saikosaponin a
  4. Saikosaponin b2
  5. Saikosaponin b1
  6. Saikosaponin d
Flow Rate 0.7 ml/min
Temperature 50 °C
Detection UV206 nm Injection Vol. 1.0 µl

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Separation of Geometrical Isomers
2.5Cholester Competitor's 2 μm C18
2.5Cholester Competitor's 2 μm C18
Condition
Column Size 3.0 mm I.D. x 75 mm Sample
  1. cis-Stilbene (0.1 mg/ml)
  2. trans-Stilbene (0.2 mg/ml)
Mobile Phase Acetonitrile : Water = 70 : 30
Flow Rate 1.0 ml/min
Temperature 40 °C
Detection UV 254 nm Injection Vol. 1.0 µl

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Comparison of Planarity Selectivity

Cholester shows greater planarity selectivity. (Planarity : o-Terphenyl < Triphenylene)

2.5Cholester Competitor's 2 µm C18
2.5Cholester Competitor's 2 μm C18
Condition
Column Size 3.0 mm I.D. x 75 mm Sample
  1. o-Terphenyl (0.05 μg)
  2. Triphenylene (0.005 μg)
o-Terphenyl Triphenylene
Mobile Phase Methanol : Water = 90 : 10
Flow Rate 1 ml/min
Temperature 40 °C
Detection UV 254 nm

See more COSMOSIL Applications

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Brochures

  • COSMOSIL Ultra-High Performance Columnpdf (1.2 MB)
  • COSMOSIL/COSMOCORE Cholester Series Application Notebook and Reference Lispdf (14 MB)
Poster at HPLC2009

ADVANTAGES OF A NOVEL STATIONARY PHASE USING 2.5 µM PARTICLES FOR ULTRA-FAST LIQUID CHROMATOGRAPHY

  (PDF 289KB)

Analysis of Natural Compounds by 2.5Cholester

  (PDF 400KB)

Ordering Information

COSMOSIL 2.5Cholester
Product Name Size Product No.  
COSMOSIL 2.5Cholester Packed Column
2.0 mm l.D. x 50 mm 09000-01 e-Nacalai
2.0 mm l.D. x 75 mm 09047-11
2.0 mm l.D. x 100 mm 09048-01
3.0 mm l.D. x 50 mm 09049-91
3.0 mm l.D. x 75 mm 09050-51
3.0 mm l.D. x 100 mm 09051-41

COSMOSIL Cholester (Particle size : 5 µm) is also available.

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COSMOSIL 2.5πNAP

  • Low back pressure (2.5 µm silica gel)
  • Naphthalene bonded stationary phase
  • Stronger π-π interactions than Phenyl columns

Improved Separation

COSMOSIL 2.5πNAP provides greater performance in separating positional isomers and other closely related compounds which are difficult to analyze with C18.

Positional Isomers of Tolunitriles
2.5πNAP Competitor's 2 µm C18
2.5πNAP Competitor's 2 µm C<sub>18</sub>
Condition
Column Size 2.0 mm I.D. x 50 mm Sample
  1. o-Tolunitrile (2.0 μg)
  2. m-Tolunitrile (2.0 μg)
  3. p-Tolunitrile (1.0 μg)
Tolunitriles 
Mobile Phase Methanol / Water = 35 : 65
Flow Rate 0.4 ml/min
Temperature 40 °C
Detection UV 254 nm

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Positional Isomers of Tocopherols
2.5πNAP Competitor's 2 µm C18
2.5πNAP Competitor's 2 µm C<sub>18</sub>
Condition
Column Size 3.0 mm I.D. x 75 mm Sample
  1. δ-Tocopherol
  2. γ-Tocopherol
  3. β-Tocopherol
  4. α-Tocopherol
Tocopherol 
Mobile Phase 2.5πNAP:
Methanol / Water = 85 : 15
Competitor's 2µm C18:
Methanol / Water = 95 : 5
Flow Rate 1.0 ml/min
Temperature 40  °C Detection UV 295 nm

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Berberine
2.5πNAP 2.5C18-MS-Ⅱ
2.5πNAP 2.5MS-II
Condition
Column Size 3.0 mm I.D. x 75 mm Sample
  1. Palmatine Hydrochloride (0.125 µg)
  2. Berberine Hydrochloride (0.125 µg)
Berberines
Mobile Phase

Methanol/ 20mmol/l Phosphate Buffer(pH2.5)
2.5πNAP = 70 : 30
2.5C18-MS-II= 40 : 60
Flow Rate 1.0 ml/min
Temperature 40 °C
Detection UV 254 nm

See more COSMOSIL Applications

Brochures

  • COSMOSIL Ultra-High Performance Columnpdf (1.2 MB)

Application Data of Tocopherols (PDF)

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Ordering Information

COSMOSIL 2.5πNAP
Product Name Size Product No.  
COSMOSIL 2.5πNAP Packed Column
2.0 mm l.D. x 50 mm 06062-91 e-Nacalai
2.0 mm l.D. x 75 mm 06051-31
2.0 mm l.D. x 100 mm 06052-21
3.0 mm l.D. x 50 mm 06054-01
3.0 mm l.D. x 75 mm 06055-91
3.0 mm l.D. x 100 mm 06057-71

COSMOSIL πNAP Packed Column (Particle size :5 µm) is also available.

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COSMOSIL 2.5HILIC

  • Ultra-High Performance using 2.5 µm particles
  • Triazole bonded stationary phase
  • Alternative selectivity to other HILIC columns
Ultra-High-Speed Analysis (Oxidation marker analysis)

COSMOSIL 2.5HILIC can be used with any conventional LC systems.

HILIC (5 µm)
(4.6 mm I.D. - 250 mm)		 2.5HILIC (2.5 µm)
(3.0 mm I.D. - 100 mm)
5 um HILIC 2.5 um HILIC
Condition
Mobile Phase

Acetonitrile/ 10mmol/l Ammonium Acetate = 80/20

 Sample
  1. Creatinine (0.1 mg/ml)
  2. 2'-Deoxyguanosine (0.1 mg/ml
  3. 8-Hydroxy-2'-Deoxyguanosine (0.1 mg/ml)
  4. 8-Hydroxy Guanosine (0.1 mg/ml)
Flow Rate 1.0 ml/min
Temperature 40 °C
Detection UV 249 nm Inj. Vol 1.0 µl

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Applications
Umami Components
Umami Compound
Adenine Nucleotide
Adenine Nucleotide
Taurine/Hypotaurine
Taurine/Hypotaurine
Haloacetic Acid
Haloacetic Acid

See more COSMOSIL Applications

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Brochures

  • COSMOSIL Ultra-High Performance Columnpdf (1.2 MB)

Ordering Information

COSMOSIL 2.5HILIC
Product Name Size Product No.  
COSMOSIL 2.5HILIC Packed Column
2.0 mm l.D. x 50 mm 11766-21 e-Nacalai
2.0 mm l.D. x 75 mm 11768-01
2.0 mm l.D. x 100 mm 11769-91
2.0 mm I.D. x 150 mm 11770-51
3.0 mm l.D. x 50 mm 11771-41
3.0 mm l.D. x 75 mm 11772-31
3.0 mm l.D. x 100 mm 11773-21
3.0 mm I.D. x 150 mm 11774-11

COSMOSIL HILIC Packed Column (Particle size :5 µm) is also available.

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Usage information

Column 2.5 µm MS-II, Cholester
I.D. (mm) 2.0 3.0
Washing method 1. Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. For example, if your mobile phase was 50:50 methanol/20 mmol/l phosphate buffer, wash with 50:50 methanol/water.

2. (If you used a buffer, always do step 1 first!) Remove adsorbed compounds and fix unstable baselines by washing with methanol and/or THF.
Storage conditions Short-term (up to a week):
Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. For example, if your mobile phase was 50:50 methanol/20 mmol/l phosphate buffer, wash with 50:50 methanol/water.

Long-term:
Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. Then, replace the solvent with 70% methanol or acetonitrile : 30% water.

In either case, tightly plug the column, and store in a cool and dry place.
Recommended flow rate 0.4 ml/min 1 ml/min
Usable pH range pH2～pH7.5
Max. pressure 30MPa
Temperature range The maximum usable temperature is 60℃. However. for regular use, please use at a constant temperature between 20°C and 50°C.
Usable solvents Any solvent that will not dissolve the silica gel (such as alkaline solutions) or cleave the stationary phase (such as very acidic solutions) is usable.
Note: For solvents with high viscosity, please watch the system pressure and keep it below 30 MPa. Also, please wash the column after using acidic mobile phases or solvents with high freezing points.
Other instructions - Buffer concentration is usually sufficient at 0.005 - 0.02 mol/L.
- Always filter mobile phases using a 0.45 µm or finer filter before use.
- Reproducibility may worsen when using a mobile phase that is more than 90% water.

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Column 2.5 µm πNAP
I.D. (mm) 2.0 3.0
Washing method 1. Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. For example, if your mobile phase was 50:50 methanol/20 mmol/l phosphate buffer, wash with 50:50 methanol/water.

2. (If you used a buffer, always do step 1 first!) Remove adsorbed compounds and fix unstable baselines by washing with methanol and/or THF.
Storage conditions Short-term (up to a week):
Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. For example, if your mobile phase was 50:50 methanol/20 mmol/l phosphate buffer, wash with 50:50 methanol/water.

Long-term:
Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. Then, replace the solvent with 70% methanol or acetonitrile : 30% water.

In either case, tightly plug the column, and store in a cool and dry place.
Recommended flow rate 0.4 ml/min 1 ml/min
Usable pH range pH2～pH7.5
Max. pressure 30MPa
Temperature range The maximum usable temperature is 60°C. However. for regular use, please use at a constant temperature between 20°C and 50°C.
Usable solvents Any solvent that will not dissolve the silica gel (such as alkaline solutions) or cleave the stationary phase (such as very acidic solutions) is usable.
Note: For solvents with high viscosity, please watch the system pressure and keep it below 30 MPa. Also, please wash the column after using acidic mobile phases or solvents with high freezing points.
Other instructions - Buffer concentration is usually sufficient at 0.005 - 0.02 mol/L.
- Always filter mobile phases using a 0.45 µm or finer filter before use.
- Acetonitrile is not recommended as a mobile phase.

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Column 2.5 µm HILIC
I.D. (mm) 2.0 3.0
Washing method 1. Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. For example, if your mobile phase was 50:50 acetonitrile/20 mmol/l phosphate buffer, wash with 50:50 acetonitrile/water.

2. Remove adsorbed compounds and fix unstable baselines: Wash with 50:50 acetonitrile/water or up to 100% water.
Storage conditions Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid.
Then, replace the solvent with 90:10 acetonitrile/water.

Tightly plug the column, and store in a cool and dry place.
Recommended flow rate 0.4 ml/min 1 ml/min
Usable pH range pH2～pH7.5
Max. pressure 30MPa
Temperature range The maximum usable temperature is 60°C. However. for regular use, please use at a constant temperature between 20°C and 50°C.
Usable solvents - Acetonitrile/water mobile phases are recommended.
Other instructions - Retention increases with increased acetonitrile concentration.
- Acetonitrile concentration should be within 0-95% (usually 50-95%).
- Retention will decrease when using methanol/water mobile phases.
- Only use HPLC-grade solvents.
- It is necessary to use salts or buffers for dissociating compounds. However, these have low solubility in the high-organic mobile phases used in HILIC mode. Phosphate buffers, widely used in reversed-phase, are not suitable for HILIC for this reason. When using salts or buffers, the acetonitrile concentration should be 70% or less. Before use, please confirm that the additives are completely soluble in the mobile phase.

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