COSMOSIL πNAP

Features

Column Image

Naphthalene bonded stationary phase
Enhanced π-π interactions
Improved selectivity for structural isomers

For information on COSMOSIL 2.5πNAP (Ultra-High Performance Column)

Product Information

Column Specifications

Comparison of π-π interactions

COSMOSIL πNAP shows stronger π-π interactions than phenyl columns. Its two fused aromatic rings retain nitrobenzene with more π electrons stronger than phenyl columns.

pinap

Applications

Tolunitrile

Condition
Column Size	4.6 mm I.D. x 150 mm
Flow Rate	1.0ml/min
Temperature	30°C
Detection	UV 254 nm
Sample	o-Tolunitrile 2.0 µg m-Tolunitrile 2.0 µg p-Tolunitrile 1.0 µg

Adrenal Cortical Hormone

Adrenal cortical hormone

Condition
Column Size	4.6 mm I.D. x 150 mm
Flow Rate	1.0ml/min
Temperature	30°C
Detection	UV 254 nm
Sample	Prednisolone 0.33 µg Hydrocortisone 0.33 µg Cortisone 0.33 µg

Berberine

Condition
Column Size	4.6 mm I.D. x 150 mm
Mobile Phase	Methanol : 20 mmol/l Phosphate Buffer (pH2.5) = 5C₁₈-MS-II 40 : 60 5PE-MS 60 : 40 πNAP 80 : 20
Flow Rate	1.0ml/min
Temperature	30°C
Detection	UV 254 nm
Sample	Palmatine chloride 0.2 µg Berberine Hydrochoride 0.2 µg

Isoflavone

Condition
Column Size	4.6 mm I.D. x 150 mm	Flow rate	1.0ml/min
Mobile Phase	A Acetonitrile / 20mmol/l Phosphate Buffer (pH 2.5)=10/90 B Acetonitrile / 20mmol/l Phosphate Buffer (pH 2.5)=50/50 B Conc. 0 → 100% 20min Linear Gradient	Temperature	30°C
		Detection	UV 260 nm
		Sample	Daidzin 0.05 µg Glycitin 0.05 µg Genistin 0.05 µg Daidzein 0.05 µg Glycitein 0.075 µg Genistein 0.025 µg

See more COSMOSIL Applications

Usage information

Column	5 µm πNAP
I.D. (mm)	2.0	3.0	4.6	10.0	20.0	28.0
Washing method	1. Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. For example, if your mobile phase was 50:50 methanol/20 mmol/l phosphate buffer, wash with 50:50 methanol/water. 2. (If you used a buffer, always do step 1 first!) Remove adsorbed compounds and fix unstable baselines by washing with methanol and/or THF.
Storage conditions	Short-term (up to a week): Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. For example, if your mobile phase was 50:50 methanol/20 mmol/l phosphate buffer, wash with 50:50 methanol/water. Long-term: Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. Then, replace the solvent with 70% methanol or acetonitrile : 30% water. In either case, tightly plug the column, and store in a cool and dry place.
Usable pH range	pH2～pH7.5
Max. pressure	20MPa			15MPa
Temperature range	The maximum usable temperature is 60°C. However. for regular use, please use at a constant temperature between 20°C and 50°C.
Usable solvents	Any solvent that will not dissolve the silica gel (such as alkaline solutions) or cleave the stationary phase (such as very acidic solutions) is usable. Note: For solvents with high viscosity, please watch the system pressure and keep it below 20 MPa. Also, please wash the column after using acidic mobile phases or solvents with high freezing points.
Other instructions	- Buffer concentration is usually sufficient at 0.005 - 0.02 mol/L. - Always filter mobile phases using a 0.5 µm or finer filter before use. - Acetonitrile is not recommended as a mobile phase. - Baseline drift may occur.

Downloads

Brochures

COSMOSIL πNAP (3.1 MB)
COSMOSIL 2.5πNAP (1.2 MB)
Applications of Tocopherols (231 KB)

Posters

Evaluation of Pentabromobenzyl Stationary Phase for Separation of Polar Compounds by Reversed-Phase Chromatography
(Poster from HPLC 2015, Geneva)
(370 KB)

References

Reference list

COSMOSIL πNAP reference list

Ordering Information

Analytical / Preparative Column (Particle Size : 5 µm)
COSMOSIL πNAP

Product Name	Size	Product No.
COSMOSIL πNAP Guard Column
	4.6 mm I.D. x 10 mm	08082-71
	10 mm I.D. x 20 mm	08087-21
	20 mm I.D. x 20 mm	08090-61
	20 mm I.D. x 50 mm	08091-51
	28 mm I.D. x 50 mm	08094-21
COSMOSIL πNAP Packed Column
	1.0 mm I.D. x150 mm	08076-61
	1.0 mm I.D. x 250 mm	08077-51
	2.0 mm I.D. x 150 mm	08078-41
	2.0 mm I.D. x 250 mm	08079-31
	3.0 mm I.D. x 150 mm	08080-91
	3.0 mm I.D. x 250 mm	08081-81
	4.6 mm I.D. x 50 mm	08083-61
	4.6 mm I.D. x 100 mm	08084-51
	4.6 mm I.D. x 150 mm	08085-41
	4.6 mm I.D. x 250 mm	08086-31
	10 mm I.D. x 150 mm	08088-11
	10 mm I.D. x 250 mm	08089-01
	20 mm I.D. x 150 mm	08092-41
	20 mm I.D. x 250 mm	08093-31
	28 mm I.D. x 250 mm	08095-11

Ultra-High Performance Column (Particle Size : 2.5 µm)　
COSMOSIL 2.5πNAP

Product Name	Size	Product No.
COSMOSIL 2.5πNAP Packed Column
	2.0 mm l.D. x 50 mm	06062-91
	2.0 mm l.D. x 75 mm	06051-31
	2.0 mm l.D. x 100 mm	06052-21
	3.0 mm l.D. x 50 mm	06054-01
	3.0 mm l.D. x 75 mm	06055-91
	3.0 mm l.D. x 100 mm	06057-71