Saccharide Separation Columns
Saccharides are not retained on standard C18 columns because of their low hydrophobicity. COSMOSIL Sugar-D and NH2-MS are specifi cally designed for separation of saccharides. COSMOSIL C18-PAQ is recommended for hydrophobic glycosides or saccharide derivatives.
Specifications
Packing Material | Sugar-D | NH2-MS |
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USP Code | L8 | |
Silica Gel | High Purity Porous Spherical Silica | |
Average Particle Size | 5 µm | |
Average Pore Size | - | approx. 120Å |
Specific Surface Area | - | approx. 300 m2/g |
Bonded Phase Stracture | - | ![]() |
Stationary Phase | Secondary/tertiary amine | Aminopropyl group |
Bonding Type | Polymeric | |
Target Substances | Monosaccharides, oligosaccharides | |
End Capping Treatment | Near-perfect Treatment | |
Carbon Content | - | approx. 4% |
Feature |
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Comparison of Retention
The conventional aminopropyl column is slightly more retentive than Sugar-D. The retention time can be adjusted by increasing the concentration of acetonitrile in the mobile phase by 5%-10% as shown below.
Condition | |||
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Column Size | 4.6 mm I.D. x 250 mm | Detection | RI |
Flow Rate | 1.0 ml/min | Sample |
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Temperature | 30°C |