Products

COSMOSIL C18-AR-300, C8-AR-300, C4 -AR-300, Ph-AR-300

Features

Column Image

  • Wide pore reversed phase column
  • 4 types of phases (octadecyl, octyl, butyl and phenyl)

Product Information

Specifications

Packing Material 5C18-AR-300 5C8-AR-300 5C4-AR-300 5Ph-AR-300
USP Code L1 L7 L26 L11
Silica Gel High Purity Porous Spherical Silica
Average Particle Size 5 µm
Average Pore Size approx. 300A
Specific Surface Area approx. 150 m2/g
Stationary Phase
Octadecyl Group
Octyl Group
Butyl Group
Phenyl Group
Bonding Type Polymeric
Main Interactions Hydrophobic Interaction Hydrophobic Interaction
π-π Interaction
End Capping Treatment Near-perfect Treatment
pH Range 1.5-7.5*
Carbon Load approx. 12% approx. 7% approx. 6% approx. 7%

*Optimal pH range of silica-based columns is between 2 and 7.5. Extreme pH may significantly decrease column lifetime.

Comparison of Separation

COSMOSIL AR-300 packed column series offer 3 types of alkyl phases and a phenyl phase.

5C18-AR-300 5C8-AR-300 5C4-AR-300 5Ph-AR-300
5C18-AR-300 5C8-AR-300 5C4-AR-300 5Ph-AR-300
Condition
Column Size 4.6 mm I.D. x 150 mm Temperature 30 °C
Mobile Phase A : 0.05%TFA - 20% Acetonitrile Detection UV 220 nm
B : 0.05%TFA - 60% Acetonitrile
B conc. 0 → 100% 20 min Linear Gradient
Flow Rate 1.0 ml/min Sample Hemoglobine, Bovine 10 µg
5C18-AR-300 5C8-AR-300 5C4-AR-300 5Ph-AR-300
Chromatogram Chromatogram Chromatogram Chromatogram
Condition
Column Size 4.6 mm I.D. x 150 mm Temperature 30 °C
Mobile Phase A : 0.05%TFA - 20% Acetonitrile Detection UV220nm, 0.64AUFS
B : 0.05%TFA - 60% Acetonitrile Sample
  1. Insulin (1.5µg)
  2. Trypsinogen (6.0µg)
  3. Transferrin (4.0µg)
  4. Trypsin Inhibitor (5.0µg)
  5. α-Chymotrypsinogen A (4.0µg)
  6. Carbonic Anhydrase (3.0µg)
B conc. 0 → 100% 20 min Linear Gradient
Flow Rate 1.0 ml/min

Applications

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References

Reference list

Ordering Information

Analytical / Preparative Column (Particle Size: 5 μm)
COSMOSIL 5C18-AR-300

Product Name Size Product No. Price
COSMOSIL 5C18-AR-300 Guard Column
4.6 mm l.D. x 10 mm 37910-11 e-Nacalai
10 mm l.D. x 20 mm 37965-11
COSMOSIL 5C18-AR-300 Packed Column
4.6 mm l.D. x 50 mm 37911-01 e-Nacalai
4.6 mm l.D. x 150 mm 37913-81
4.6 mm l.D. x 250 mm 37914-71
10 mm l.D. x 150 mm 37917-41
10 mm l.D. x 250 mm 37918-31
20 mm l.D. x 150 mm 37919-21
20 mm l.D. x 250 mm 37920-81

COSMOSIL 5C8-AR-300

Product Name Size Product No. Price
COSMOSIL 5C8-AR-300 Guard Column
4.6 mm l.D. x 10 mm 37950-91 e-Nacalai
10 mm l.D. x 20 mm 34464-61
COSMOSIL 5C8-AR-300 Packed Column
4.6 mm l.D. x 50 mm 37951-81 e-Nacalai
4.6 mm l.D. x 150 mm 37953-61
4.6 mm l.D. x 250 mm 37954-51
10 mm l.D. x 150 mm 34345-21
10 mm l.D. x 250 mm 34247-11
20 mm l.D. x 150 mm 05861-51
20 mm l.D. x 250 mm 34364-71

COSMOSIL 5C4-AR-300

Product Name Size Product No. Price
COSMOSIL 5C4-AR-300 Guard Column
4.6 mm l.D. x 10 mm 37955-41 e-Nacalai
10 mm l.D. x 20 mm 05862-41
COSMOSIL 5C4-AR-300 Packed Column
4.6 mm l.D. x 50 mm 37956-31 e-Nacalai
4.6 mm l.D. x 150 mm 37958-11
4.6 mm l.D. x 250 mm 37959-01
10 mm l.D. x 150 mm 34249-91
10 mm l.D. x 250 mm 38047-11
20 mm l.D. x 150 mm 34477-01
20 mm l.D. x 250 mm 38048-01

COSMOSIL 5Ph-AR-300

Product Name Size Product No. Price
COSMOSIL 5Ph-AR-300 Guard Column
4.6 mm l.D. x 10 mm 37960-61 e-Nacalai
10 mm l.D. x 20 mm 34268-41
COSMOSIL 5Ph-AR-300 Packed Column
4.6 mm l.D. x 50 mm 37961-51 e-Nacalai
4.6 mm l.D. x 150 mm 37963-31
4.6 mm l.D. x 250 mm 37964-21
10 mm l.D. x 150 mm 05865-11
10 mm l.D. x 250 mm 34267-51
20 mm l.D. x 150 mm 05866-01
20 mm l.D. x 250 mm 34468-21

(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze