Our paper covering an experimental method for analyzing short-, medium-, and long-stranded RNAs has been published in the Royal Society of Chemistry's Analytical Methods, and was selected as a front cover article.

Title:

Separation and purification of short-, medium-, and long-stranded RNAs by RP-HPLC using different mobile phases and C₁₈ columns with various pore sizes

Authors:

Makoto Ozaki, Tomomi Kuwayama, Motoshi Shimotsuma, Tsunehisa Hirose

Journal reference:

Analytical Methods. 2024;16:1948-1956.
DOI:https://doi.org/10.1039/D4AY00114A

Featured on Front Cover

Summary:

In the analysis of nucleic acids, most researchers focus on either short-stranded oligonucleotides or long-stranded nucleic acids; there had not been any comprehensive report discussing analysis conditions for short- and long-stranded nucleic acids. In this report, we have evaluated conditions for each length category in terms of HPLC column pore size and mobile phase composition. In addition, using these optimized conditions, we separated medium- (several hundred nt) and long-strand (several thousand nt) nucleic acids from their respective impurities and established a method for preparative purification.

Front cover art: Illustration of synthetic long-stranded nucleotides being separated from their impurities by COSMOSIL RNA-RP1 with super wide pores.

Products used in this article:

• COSMOSIL RNA-RP1
• COSMOCORE 2.6C₁₈ (used for short-stranded oligonucleotides)