Application of the Month  July 2017

Identifying vitamins D2/D3 and their 25-OH metabolites and C3 epimers in a single LC-MS run

Vitamin D2 and D3 isocratic separation under 3 minutes using 100% MeOH

25(OH) Vitamin D2 and D3 metabolites and C-3 epimers were baseline separated under isocratic conditions

All six vitamin D and associated metabolites were separated in a single HPLC gradient run

 

 

Vitamin D Structures

 

 

Vitamin D Separation

 

 

Vitamin D Metabolite and Epimer LC-MS Data

 

 

Vitamin D Metabolite and Epimer Separation - Flow Rate Comparison

 

 

Vitamin D, Metabolite, and Epimer Separation

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