Products

COSMOCORE 2.6PBr

Features

  • Separate hydrophilic compounds in reversed-phase conditions
  • Retain hydrophilic compounds longer than C18
  • Greater sample loading capacity than HILIC
  • High performance similar to sub-2 µm particles with lower back pressure

Suitable Samples

  • Hydrophilic compounds
  • Nucleic acids and derivatives
  • Surfactants
  • Glycosides
  • Peptides

>> Information on COSMOCORE 2.6C18

>> Information on COSMOCORE 2.6Cholester

>> Information on COSMOSIL 5PBr (fully porous particles)

Product Information

About Core-Shell Particles

About Core-Shell Particles

Column Specifications

Column Specifications

Separation of Hydrophilic Compounds (low retention on C18)

COSMOSIL PBr retains hydrophilic compounds stronger than C18 columns under the same reversed-phase conditions.

Nucleic Acid Metabolites

Nucleic Acid Metabolites_Application

Conditions
Column size 2.1mmI.D.-100mm Sample 
  1. Allantoin (2.0 mg/ml)
  2. Guanine (0.05 mg/ml)
  3. Hypoxanthine (0.05 mg/ml)
  4. Uric Acid (0.1 mg/ml)
  5. Xanthine (0.2 mg/ml)
  6. Adenosine (0.2 mg/ml)
  7. Inosine (0.1 mg/ml) 
Mobile phase Methanol/ 100mmol/l Phosphate buffer(pH2.5) = 10/90
Flow rate 0.4 ml/min
Temperature 40°C
Detection UV 240 nm Inj. Vol 1.0 µl

Malonyl CoA, CoA, Acetyl CoA

Malonyl CoA, CoA, Acetyl CoA_Application

Conditions
Column size 2.1 mm I.D. - 100 mm Sample
  1. Malonyl CoA (0.5 mg/ml)
  2. CoA (0.5 mg/ml)
  3. Acetyl CoA (0.5 mg/ml)
Mobile phase Acetonitrile/ 20mmol/l Phosphate buffer(pH7.0) = 5/95
Flow rate 0.4 ml/min
Temperature 40°C
Detection UV 260 nm Inj. Vol 1.0 µl

Separation of Hydrophilic Compounds (compounds with similar hydrophobicity)

COSMOCORE 2.6PBr can separate compounds with similar hydrophobicity, utilizing several kinds of molecular interactions, including dispersion force generated by the bromine atoms.

ATP，ADP，AMP

ATP，ADP，AMP_Application

Conditions
Column size 2.1 mm I.D. - 100 mm Sample
  1. ATP (0.5 mg/ml)
  2. ADP (0.8 mg/ml)
  3. AMP (0.8 mg/ml)
Mobile phase 20mmol/l Phosphate buffer(pH7.0)
Flow rate 0.4 ml/min
Temperature 40°C
Detection UV 260 nm Inj. Vol 0.5 µl

Vitamin B3 (Nicotinic Acid, Nicotinamide)

Vitamin B3 (Nicotinic Acid, Nicotinamide)_Application

Conditions
Column size 2.1 mm I.D. - 150 mm Sample
  1. Nicotinic Acid (0.4 mg/ml)
  2. Nicotinamide (0.6 mg/ml)
Mobile phase 10mmol/l Phosphate buffer(pH7.0)
Flow rate 0.4 ml/min
Temperature 40°C
Detection UV 260 nm Inj. Vol 0.5 µl

Thiamine Pyrophosphate, Thiamine

Thiamine Pyrophosphate, Thiamine_Application

Conditions
Column size 2.1 mm I.D. - 150 mm Sample
  1. Thiamine Pyrophosphate (0.4 mg/ml)
  2. Thiamine (0.4 mg/ml)
Mobile phase 100mmol/l Phosphate buffer(pH2.5)
Flow rate 0.4 ml/min
Temperature 40°C
Detection UV 245 nm Inj. Vol 0. 5 µl

Separation Mechanism

Dispersion force (instantaneous dipole-induced dipole force)

London dispersion force is a weak intermolecular force that results from dipoles temporarily induced by random unsymmetrical electron positions in two adjacent atoms, also known as instantaneous dipole?induced dipole force. It is present in all molecules regardless of polarity. Compounds with high polarizability have stronger dispersion force.

Compounds with stronger dispersion force

  • Larger and heavier molecules
  • Molecules with larger and heavier atoms (e.g. from weakest to strongest in halogens, F2, Cl2, Br2, and I2)
  • Molecules with delocalized electrons and resonance (e.g. aromatic compounds)

⇓

COSMOSIL PBr column is packed with pentabromobenzyl-bonded silica that enables separation by dispersion force interaction.

Difference between Methanol and Acetonitrile Mobile Phases

Comparison of Separation Ability

Acetonitrile is frequently used in HPLC to reduce backpressure. However, the π electrons in acetonitrile interfere with the dispersion force interaction between the sample and the stationary phase. Therefore, we recommend using methanol as the organic solvent.

Comparison of Separation Ability_Application

Conditions
Column size 2.1 mm I.D. - 100 mm Sample
  1. Stevioside (5.0 mg/ml)
  2. Rebaudioside A
Flow rate 0.4 ml/min
Temperature 40°C
Detection UV 210 nm Inj. Vol 1.0 µl

Comparison to HILIC

Both columns are suitable for analysis of hydrophilic compounds, but they have different properties.

PBr HILIC
Separation
Mode		 Reversed Phase Hydrophilic Interaction (HILIC)
Features
  • Simple mobile phase conditions compared to HILIC.
  • Strong eluent: organic (methanol) Weak eluent: water
  • Low peak distortion with water-based samples; usable with large injection volumes of dilute samples.
  • Some hydrophilic compounds are not retained well.
  • Retains hydrophilic compounds that would not be retained by C18 columns.
  • Strong eluent: water Weak eluent: organic (acetonitrile)
  • Peak distortion occurs with large volumes of samples dissolved in water.

Applications

Separation of Hydrophilic Compounds (low retention on C18)

Water-Soluble Vitamins

Water-Soluble Vitamins_Application

Conditions
Column size 2.1 mm I.D.-150 mm
Mobile phase A; 20mmol/l Phosphate buffer(pH2.5)
B; Methanol/ 20mmol/l P hosphate buffer(pH2.5) = 60/40
B conc. 0% (0-1min), 0→80% (1→5min), 80→100% (5→9min)
Flow rate 0.4 ml/min
Temperature 40°C
Detection UV 220 nm
Sample
  1. Vitamin B1 [Thiamine] (0.25 mg/ml)
  2. Vitamin C [Ascorbic Acid] (1.0 mg/ml)
  3. Vitamin B3 [Nicotinic Acid] ( 0.04 mg/ml)
  4. Vitamin B3 [Nicotinamide] ( 0.06 mg/ml)
  5. Vitamin B6 [Pyridoxine] (0.25 mg/ml)
  6. Vitamin B5 [Pantothenic Acid] (2.0 mg/ml)
  7. Vitamin B9 [Folic Acid] (0.2 mg/ml)
  8. Vitamin B7 [Biotin] (3.0 mg/ml)
  9. Vitamin B12 [Cyanocobalamin] (0.25 mg/ml)
  10. Vitamin B2 [Riboflavin] ( 0.2 mg/ml)
Inj. Vol 1.25 µl

Separation of Hydrophilic Compounds (Glycosides)

Glycosides with identical aglycones but different glycosyl groups can also be separated.

UDP Glycosides

UDP Glycosides_Applications

Conditions
Column Size 2.1 mm I.D.-150 mm Sample
  1. UDP (0.8 mg/ml)
  2. UDP-Galactose (0.8 mg/ml)
  3. UDP-Glucose (0.8 mg/ml)
Mobile Phase 100 mmol/l Phosphate buffer(pH7.0)
Flow Rate 0.4 ml/min
Temperature 40°C
Detection UV 260 nm Inj. Vol: 0.5 µl

Arbutin and Hydroquinone

Arbutin and Hydroquinone_Applications

Conditions
Column size 2.1 mm I.D. - 100 mm Sample
  1. Arbutin (1.33 mg/ml)
  2. Hydroquinone (0.67 mg/ml)
Mobile phase Acetonitrile/ 20mmol/l Phosphate buffer(pH2.5)
Flow rate 0.4 ml/min
Temperature 40°C
Detection UV 290 nm Inj. Vol 0.75 µl

icon See more COSMOSIL Applications

Usage information

Column 2.6 COSMOCORE PBr
I.D. (mm) 2.1 3 4.6
Washing method 1. Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. For example, if your mobile phase was 50:50 methanol/20 mmol/l phosphate buffer, wash with 50:50 methanol/water.

2. (If you used a buffer, always do step 1 first!) Remove adsorbed compounds and fix unstable baselines by washing with methanol and/or THF.
Storage conditions Short-term (up to a week):
Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. For example, if your mobile phase was 50:50 methanol/20 mmol/l phosphate buffer, wash with 50:50 methanol/water.

Long-term:
Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. Then, replace the solvent with 70% methanol or acetonitrile : 30% water.

In either case, tightly plug the column, and store in a cool and dry place.
Usable pH range pH 2-7.5
Max. pressure 60MPa
Temperature range The maximum usable temperature is 60°C. However. for regular use, please use at a constant temperature between 20°C and 50°C.
Usable solvents Any solvent that will not dissolve the silica gel (such as alkaline solutions) or cleave the stationary phase (such as very acidic solutions) is usable.
Note: For solvents with high viscosity, please watch the system pressure and keep it below 60 MPa. Also, please wash the column after using acidic mobile phases or solvents with high freezing points.
Other instructions - Buffer concentration is usually sufficient at 0.005 - 0.02 mol/L.
- Always filter mobile phases using a 0.45 µm or finer filter before use.
- Acetonitrile is not recommended as a mobile phase.

Downloads

Brochures

Posters

  • Profiling of 11 Cannabinoid Mixture by PBr HPLC column (Poster from AOAC2017, USA)pdf(0.3 MB)
  • Selectivity Comparison of BromoBenzyl(PBr)
    to FluoroPhenyl(PFP) Core-Shell HPLC Columns (Poster from ASMS2017, USA)
    pdf(0.3 MB)

Ordering Information

Analytical Column (Particle size : 2.6 µm)

COSMOCORE 2.6PBr Column
Product Name Size Product No. Price
COSMOCORE 2.6PBr
2.1 mm I.D. x 30 mm 13692-21 Inquire
Now
2.1 mm I.D. x 50 mm 13693-11
2.1 mm I.D. x 75 mm 13694-01
2.1 mm I.D. x 100 mm 13695-91
2.1 mm I.D. x 150 mm 13697-71
3.0 mm I.D. x 30 mm 13698-61
3.0 mm I.D. x 50 mm 13699-51
3.0 mm I.D. x 75 mm 13700-01
3.0 mm I.D. x 100 mm 13701-91
3.0 mm I.D. x 150 mm 13703-71
4.6 mm I.D. x 30 mm 13705-51
4.6 mm I.D. x 50 mm 13712-51
4.6 mm I.D. x 75 mm 13714-31
4.6 mm I.D. x 100 mm 13715-21
4.6 mm I.D. x 150 mm 13719-81
4.6 mm I.D. x 250 mm 13734-71

Other sizes may be available.

>> For UHPLC (COSMOCORE) fittings and adapters, please see this page.