COSMOSIL C₁₈-AR-II

Features

Column Image

Features strong acid resistance
Suitable for acid compounds and peptides

Product Information

Column Specifications

Acid Resistance

The COSMOSIL 5C18-AR-II packed column features a polymeric type of C18 reversed phase material. The acidic resistance of COSMOSIL 5C18-AR-II is much improved compared with commercially available monomeric type octadecyl stationary phases. It retains high performance even in case of acidic mobile phases commonly used to separate acidic compounds and peptides.

Figure.
Decomposition test in 0.1% Trifluoroacetic Acid solution at 60°C .
Capacity factor (k') = Naphthalene,
Mobile phase: 70% methanol

Applications

Peptides

Condition
Column Size	4.6 mm I.D. x 150 mm
Mobile Phase	A; 0.05%TFA-H2O B; 0.05%TFA-Acetonitrile B conc. 10→40% 20min Linear gradient
Flow Rate	1.0 ml/min
Temperature	30°C
Detection	UV220nm
Sample	Oxytocin (0.8µg) Angiotensin II(Human) (0.8µg) Angiotensin I(Human) (0.8µg) Substance P (0.8µg)

Organic Acids

Condition
Column Size	4.6 mm I.D. x 150 mm
Mobile Phase	Methanol/ 20mmol/l Phosphoric Acid = 20/80
Flow Rate	1.0 ml/min
Temperature	30°C
Detection	UV254nm, 0.5AUFS
Sample	Gallic Acid (0.63µg) Protocatechuic Acid (0.63µg) Gentisic Acid (0.63µg) Phthalic Acid (0.63µg)

Salicylic Acid Esters

Condition
Column Size	4.6 mm I.D. x 150 mm
Mobile Phase	Acetonitrile/ H2O = 70/30
Flow Rate	1.0 ml/min
Temperature	30°C
Detection	UV254nm, 0.2AUFS
Sample	Methyl Salicylate (2.3µg) Ethyl Salicylate (2.6µg) n-Propyl Salicylate (2.3µg) n-Butyl Salicylate (2.6µg)

Parabens

Condition
Column Size	4.6 mm I.D. x 150 mm
Mobile Phase	Acetonitrile/ H2O = 50/50
Flow Rate	1.0 ml/min
Temperature	30°C
Detection	UV254nm, 0.12AUFS
Sample	Methyl p-Hydroxybenzoate (0.125µg) Ethyl p-Hydroxybenzoate (0.125µg) n-Propyl p-Hydroxybenzoate (0.125µg) Butyl p-Hydroxybenzoate (0.125µg)

LC/MS Applications

AR-II-LCMS

See more COSMOSIL Applications

Usage information

Column	5 µm C18-AR-II
I.D. (mm)	1.0	2.0	3.0	4.6	6.0	10.0	20.0	28.0
Washing method	1. Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. For example, if your mobile phase was 50:50 methanol/20 mmol/l phosphate buffer, wash with 50:50 methanol/water. 2. (If you used a buffer, always do step 1 first!) Remove adsorbed compounds and fix unstable baselines by washing with methanol and/or THF.
Storage conditions	Short-term (up to a week): Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. For example, if your mobile phase was 50:50 methanol/20 mmol/l phosphate buffer, wash with 50:50 methanol/water. Long-term: Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. Then, replace the solvent with 70% methanol or acetonitrile : 30% water. In either case, tightly plug the column, and store in a cool and dry place.
Usable pH range	pH1.5～pH7.5 (pH 2-7.5 recommended)
Max. pressure	20MPa					15MPa
Temperature range	The maximum usable temperature is 60°C. However. for regular use, please use at a constant temperature between 20°C and 50°C.
Usable solvents	Any solvent that will not dissolve the silica gel (such as alkaline solutions) or cleave the stationary phase (such as very acidic solutions) is usable. Note: For solvents with high viscosity, please watch the system pressure and keep it below 20 MPa. Also, please wash the column after using acidic mobile phases or solvents with high freezing points.
Other instructions	- Buffer concentration is usually sufficient at 0.005 - 0.02 mol/L. - Always filter mobile phases using a 0.5 µm or finer filter before use. - Reproducibility may worsen when using a mobile phase that is more than 90% water.

Downloads

Brochure

COSMOSIL 5C₁₈-AR-II pdf (659 KB)

References

Reference list

COSMOSIL C18-AR-II reference list

Ordering Information

Analytical Column (Particle Size : 5 µm)
COSMOSIL 5C₁₈-AR-II

Product Name	Size	Product No.
COSMOSIL 5C₁₈-AR-II Guard Column/Guard Cartridge
	4.6 mm I.D. x 10 mm	38141-61
	4.6 mm I.D. x 10 mm Cartridge	38008-89
	10 mm I.D. x 20 mm	38148-91
	20 mm I.D. x 20 mm	34458-51
	20 mm I.D. x 50 mm	34479-81
	28 mm I.D. x 50 mm	34363-81
COSMOSIL 5C₁₈-AR-II Packed Column
	1.0 mm I.D. x 50 mm	02955-21
	1.0 mm I.D. x 150 mm	02951-61
	2.0 mm I.D. x 30 mm	05098-71
	2.0 m m I.D. x 50 mm	34400-81
	2.0 mm I.D. x 100 mm	34469-11
	2.0 mm I.D. x 150 mm	37992-51
	2.0 mm I.D. x 250 mm	05272-71
	3.0 mm I.D. x 100 mm	05791-71
	3.0 mm I.D. x 150 mm	38028-61
	3.0 mm I.D. x 250 mm	38029-51
	4.6 mm I.D. x 30 mm	05877-61
	4.6 mm I.D. x 50 mm	38142-51
	4.6 mm I.D. x 100 mm	38143-41
	4.6 mm I.D. x 150 mm	38144-31
	4.6 mm I.D. x 250 mm	38145-21
	6.0 mm I.D. x 150 mm	38146-11
	6.0 mm I.D. x 250 mm	38147-01
	10 mm I.D. x 50 mm	05369-21
	10 mm I.D. x 150 mm	34350-41
	10 mm I.D. x 250 mm	38149-81
	20 mm I.D. x 150 mm	34316-01
	20 mm I.D. x 250 mm	38150-41
	28 mm I.D. x 250 mm	34362-91

4.6 mm I.D. x 10 mm Cartrige contains 3 PKG. Cartridge Holder (Product number: 38009-79) is required.

Preparative Column (Particle Size : 15 µm)
COSMOSIL 15C₁₈-AR-II

Product Name	Size	Product No.
COSMOSIL 15C₁₈-AR-II Guard Column
	28 mml.D. x 50 mm	38030-11
	50 mml.D. x 50 mm	38057-81
COSMOSIL 15C₁₈-AR-II Packed Column
	28 mml.D. x 250 mm	37978-51
	50 mml.D. x 250 mm	38058-71
	50 mml.D. x 500 mm	05884-61